Virus replication Flashcards
What chain direction is RNA?
5’ to 3’
What direction are -ve strand genomes?
3’ to 5’
Where are simple posiitive strand RNA viruses translated?
Cytoplasm
Step by step of host cell protein synthesis
Eukaryotic initiation factor complex (eIF4F) recognises the 5’ cap structure
eIF4F recruits 43S subunit
43S Scans the mRNA until AUG, the recruits 60S
Forms complex
What virus contains a pseudoknot?
Hep C
Where are IRES elements found?
5’ end of many positive strand RNA viruses
How are IRES useful experimentally
Allow for one piece of mRNA to produce different proteins
What type of helicase is necessary for the translation of some mRNAs?
RNA helicase A
How does RNA helicase A help in translation?
Unwinds RNA secondary structures, assists folding of RNA/RNP complexes. Act as RNA chaperones.
What is Nsp14 used for in coronavirus replication?
Exonuclease domain proofreads nascent RNA strand and excises mis incorporated nucelotides.
What is nsp14’s cofactor?
nsp10
What are double membraned vesicles for?
Concentrate replication components. Protect against innate immune response and RNA is a PAMP.
Cellular proteins are important for RNA virus replication
Phosphatidylinositol 4-kinase (PI4K)
Production of phosphatidylinositol 4-phosphate (PI4P)
Involved in membrane trafficking and curvature.
What is an inhibitor of PI4K?
PIK93
