Virus Hunting

Question 1

Poriteas Compresa case study

Answer 1

Detected first Herpesvirus in invertebrates

Thought coral bleaching due to environmental stressors

Collected Hawaii coral and put under different stressors

Increased Temp
Detergent
pH lowered
Nutrient level increased

Virus was isolated and NGS was used to be able to identify

Question 2

Acute flaccid paralysis in children ev

Answer 2

Isolated Virus from 3 SE Asian children stools from AFP

454 revealed more viruses per sample than danger

23% of sequences mapped to known Virus factor

Specific viral species picornaviruses and rotaviruses

Unknown viruses mapped to known sequences but some novel

Question 3

Murkle cell polya virus

Answer 3

Only discovered In 2008 by digital Transcription subtraction vs the human genome

Known to be present in 80% of MCC

Question 4

Tropical seawater

Answer 4

Water samples from Hawaiian bays surveyed using pyroseq.

Identified picornaviriad as most common RNA virus 95% were similar to picornavirades

Assembled 6 picorna genomes from metagenomic libraries

Encoded 1 or 2 ORF similar to conserved domain of structural or non-structural proteins

Question 5

GVFI

Answer 5

Non profit organisation

Tracks mainly zoonosis that could jump species barrier and become pandemic