Virus Host Cell Interactions Flashcards
Viruses are what kind of parasites? How do they multiply?
obligate intracellular parasites ; with the help of the genetic and metabolic machinery from the host cell
Herpes virus simplex can grow in what two areas of the embryonated chick egg?
chorioallantoic membrane, yolk sac
these contain cells that have the receptor for this virus
Mumps virus can grow in what area of the embroyanted chick egg?
amniotic membrane and the allantoic cavity
The influenza virus grow primarily where in the embryonated chick egg?
allantoic cavity
Which virus cause bronchiolotis?
respiratory synctial virus
All cell lines can provide an effective platform for detection of viruses. True or false?
FAALLLLLSSSE!!!!
Not all cell lines can provide an effective platform for the detection of viruses.
Human epithelial cell lines can be used to detect what virus? Human fibroblasts cell lines? Green monkey kidney cell lines?
Human epithelial cell lines: respiratory syncytial virus (RSV)
Human fibroblasts cell lines: cytomegalovirus (CMV), varicella-zoster virus (VZV), rhinoviruses
Green monkey kidney cell lines: enteroviruses, respiratory viruses, herpes simplex virus (HSV)
What are the 3 kinds of cells of tissue cultures that are used to examine viruses in vitro?
Primary cell culture from DIFFERENTIATED cells directly derived from a tissue; has limited life span
Diploid cell lines that come from DEdifferentiated, dipolid cells which survive more passages than primary cell lines but still eventually die
Continuous cell lines: heterploid cells which MOSTLY come from tumors and allow the cells to be passaged many times
HeLa cell lines is what kind of tissue culture?
Continous cell line
was derived from a cervical carcinoma from Henrietta Lacks
We can detect viral multiplication by 3 different outcomes which are?
Cytopathic Effect (CPE): microscopically visible changes in cultured cells; nature of the morphological changes
Hemadsorption: hemagluttinin of the virus is inserted into the cell membrane; a suspension of RBCs can be placed into the culture and the RBCs will adhere to the infected cells, thus islands of infected cells in a culture you can determine the number of cells that are infected at a given time
Interference: can detect viral multiplication INDIRECTLY as infection with a virus prevents an infection by a test (challenge) virus
Hemadsorption is a good technique to observe viral replication in which kind of viruses?
influenza in orthomyxovirus family
paramyxoviruses: parainfluenza, and mumps
CPE: cytopathic effect is usually a secondary results of changes in what?
in the host metabolism caused by viral replication
-viruses may halt or alter host cell DNA synthesis, transcription, and/or protein synthesis (translation)
Presence of polykaryocytes or multinucleated cells is a cytopathic effect (CPE) in primary rabbit kidney cells due to which virus?
Herpes simplex virus-1
-respiratory syncytial virus, measles virus, herpes zoster virus does NOT GROW in rabbit kidney cells
Measles form multi-nucleated cells or syncytium or polykaryocytes in what kind of cells and is indicative of what disease?
in vero cells which are green monkey kidney cells; pneumonia
HSV-1 causes syncytium but in rabbit kidney cells
RSV causes syncytium but in human epithelial cells (HEP-2)
What is the advantage of using the green fluorescent protein as opposed to the cytopathic effect (CPE) to detect the presence of a virus?
it causes a FASTER identification as opposed to the CPE in which we have to wait to develop
green fluorescent protein which has been engineered by recombinant method encompasses a green fluorescent that when exposed to the UV light, fluoresces green.
