Virus Flashcards
DNA virus
Enveloped
HHP
Hepadna (Hep B)
Herpes (1-8)
Pox (a/e chicken pox, vaccinia Variola MC)
DNA
Non enveloped
PAP
Papova (HPV, JC, SV40)
Adeno
Parvo (B19)
Smallest overall
Parvo B19
Pox symmetry
Only virus with Complex symmetry
RNA enveloped
RFT
Retro (HIV, HTLV)
Flavi (HepC)
Toga (chikunginia, rubella)
Arena - lassa Bunya - hanta Orthomyxo - influenza Paramyxo LARGEST RNA Rhabdo Rabies Filo ebola Corona Delta - HepD
Largest virus overall
Pox virus
KFD JE Dengue Yellow fever Zika HEP C
Arbovirus 🦔
⬇️
FLAVI
RNA non enveloped
PCR
Picorna SMALLEST RNA Hep A Polio
Calci - Hep E
Reo - rota
Picorna
- Entero
- Rhino
Entero -
Polio
Cocksackie
Hep A
Rhino 🤷🏻♀️
RNA or DNA
RNA and DNA
Or VIRUS
And BACTERIA
Symmetry is given by?
Capsid (protein) around the NA
Icosahedral
HH
PAP
RFT
PCR
Complex
Pox and
Helical
Extra enveloped RNA list
Segmented virus
RAMBO Rota/reo 11 - HIGHEST Adeno 2 M Bunya 3 Orthomyxo 8
Rest ALL non segmented
ds RNA
Reovirus
ss DNA
Parvo
All have linear NA except helical NA by?
DNA
- Hep B
- HPV
RNA
- Arena
- Bunya
HA - hemagglutination
NA - elution by binding to SIALIC ACID RS ON RBC
HA - all virus NA - IMP Influenza Parainfluenza Mumps
RNA negative 🎞 sense strand
Arena Bunya Orthomyxo Paramyxovirus Rhabdo Filo
Intranuclear
Intracytoplasmic
Both
Intranuclear COWDRY
Intracytoplasmic CPR
Both MEASELS
Intranuclear
HAY BAP
Cowdry A - Herpes and Yellow fever
B - Adeno and Picorna/polio
Intra cytoplasmic
CPR
Chlamydia
Pox
Rabies
Torres bodies
Yellow fever
Owl eye 👁
CMV
Lipshutz
HSV
Paschen
Variola
Guarneri
Vaccinia
HPaterson
MC
Bollinger
Fowl pox
Warthin finkedly
MEASELS
Negri
Rabies
Adsorption Penetration Uncoating - reveals NA Biosynthesis Assembly Mature Release
Steps of viral replication
Penetration via Endocytosis Pino Phagocytosis EXCEPT
Hiv - membrane fusion
Bacteriophage - never enters
Release by
Lysis
Budding
Lysis - non enveloped
Budding - enveloped (HOST DERIVED )
Animal inoculation
Embryonated egg Cx
Tissue Cx
Viral culture methods
NEED LIVE CELLS
Animal inoculation MICE mc
Embryonated egg Cx
Tissue Cx MC
Sucking mice
- cerebral
- foot pad
Cerebral - Coxsackie
A - flaccid paralysis
B - spastic
Foot pad - Mleprae
Parts of embryonated egg
Yolk sac
Amniotic cavity
Allantois (C shape)
CAM
CAM
HSV
Pox mc
Allantois
Amniotic cavity
Influenza
Mumps
Yolk sac
HSV
Arbo
Chlamydia
Ricketssia
Vaccines cultured in
Allantoic cavity
Allantoic cavity (large)
YIR
Yellow fever
Influenza
Rabies
Tissue culture
- organ Cx
- explant
- cell Cx
- organ Cx Tracheal ring
- explant Minced adenoid
- cell Cx mc
•primary
•diploid
•continuous
Chick embryo fibroblast 🐥
Rhesus monkey 🐒 kidney
Human embryonic kidney
Primary cell Cx
Human diploid fibroblast (lung )
Diploid cell Cx
Lung
WI38
MRC5
Hela Hep 2 Mc coy BHK Vero MC
Continuous cell line
African green monkey 🐒 kidney cells
Vero
Detection of viral growth
Cytopatho effect
Viral interference (one virus with another)
IF
CPE of Entero
Rapid crenation
CPE of MEASELS
Syncitium formation
CPE of HSV
Ballooning of cells
CPE of SV40
Vacuolation
CPE of adeno
Grape like clusters 🍇
GRANULOMA CLUMPS
Virions vs Viroids
Virions - infectious virus
Viroid- plants 🪴 infections
Viral assay
Quantal
Quantitative
Quantal - presence or absence
EM PCR HA
QUANTITATIVE- exact no of virions
POCK - CAM
PLAQUE - Tissue Cx Monolayer Cx
Types of interferons
iF- cytokines (human proteins)
Host specific not virus 🦠 specific
Type 1 INF alpha and beta
2 INF gamma
3 no name
INF alfa - leukocytes
INF beta - fibroblasts
Innate immunity
Both TYPE 1
INF alpha and beta MOA
Induces host cell ⬇️ TIPS translational inhibition proteins ⬇️ Aborts disease
Acquired immunity
5-7 days to develop Ab
⬇️
Activated T cells
INF gamma
Type 2
IgM disappears in
5-6 months
INF Alfa and beta ⬇️ Stimulate NK cells ⬇️ INF gamma
🧗🏻♀️
Virus infects SPECIFIC bacteria
Bacteriophage via bacterial identification
C diph
Transduction
Phage cycles
Phage injects Nucleic acid
⬇️
1. Lysogenic
2. Lytic
NA of phage incorporated into bac
as prophage
⬇️
Bacteria has property of phage
Lysogenic conversion
12 Live vaccines
BCG, OPV, Rota and MMR
Small pox, Chicken pox, typhoid and cholera
Flavi😷: JE, Dengue FDA ❌, yellow fever, influenza (intranasal spray)
Culex (2)
JE
FILARIA
Quadrivalent
Penta
Hexa ?
Quad- DPT and Hib
Penta - DPT Hib and Hep B
Hexa - Rota