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MCB LAB > VIRTUAL MICROSCOPY > Flashcards

VIRTUAL MICROSCOPY Flashcards

1
Q

provides a self-guided exploration of a common laboratory microscope, its care and usage, and general information about the equipment and its components.

A

Virtual Microscope

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2
Q

each lens has a specific, color-coded band that denotes its resolution:

red -
yellow -
blue -
white -

A

red - 4X
yellow - 10X
blue - 40X
white - 100X

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3
Q

total magnification formula

A

eyepiece x objective

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4
Q

things you can see in an immersion oil lens (5)

A
  • nosepiece threading
  • manufacturer (label)
  • objective specifications (label)
  • magnification color code
  • spring load retractable front lens
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5
Q

is typically used with higher powered lens such as 100X

A

oil

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6
Q

T or F

Never touch the lenses with your fingers. Oil from your hands can smudge the lens and in some cases even leave scratches. Use only the appropriate lens tissues to avoid damaging the lens.

A

True

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7
Q

specially-designed lens, with an iris-type aperture, and mounted under the stage, moves vertically to adjust the beam of light entering the lens system. Changing the size of the iris and adjusting its position in relation to the stage controls the diameter and focal point of the cone of light as it passes through the specimen.

A

Abbe Condenser

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8
Q

are used to correct the bending or refraction of light as it passes through the prisms or lenses in the microscope. Each has different indexes of refraction to realign certain colors of light at the focal point, result in a much clearer image.

A

Achromatic lenses

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9
Q

area of the microscope between the tube and base

A

arm

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10
Q

this stand holds the microscope body, clams to a table, and provides movement in three dimensions.

A

articulated arm

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11
Q

features a head with two eyepiece lenses, one eyepiece for each eye.

A

binocular microscope

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12
Q

refers to he main section of the microscope, minus the stand (base) or any illuminators

A

body

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13
Q

is an adapter for use with a video camera. In general the lens from the camera is removed and replaced with an adapter which then connects to the trinocular port of the microscope

A

C-mount

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14
Q

This focusing system features the coarse and fine focus knobs on a single rotation axis.

A

Coaxial focus

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15
Q

Mounted in or below the stage, this lens focuses or “condenses” the light onto a specimen.

helps increase illumination and resolution

A

condenser lenses

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16
Q

used on low-power microscopes, this circular opaque plate is placed on the stage, and can be flipped between a white or black side depending on the coloration of the specimen

A

contrast plate

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17
Q

most useful at higher powers, it is typically a five-hole disc housed under the stage that adjusts the amount of light passing through the stage opening. Each opening is a different diameter controlling how much light passes through to help properly illuminate the specimen while also increasing contrast and resolution

A

diaphragm

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18
Q

This German standard for the manufacture of microscope lenses features a uniform thread and works with a 160mm tube length

A

DIN optics

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19
Q

used on microscopes with a binocular head, this adjustment allows each eyepiece to be focused independently to compensate for the difference in vision between your two eyes

A

Diopter adjustment

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20
Q

generally found on high-power microscopes this unit features a single eyepiece on one side and an additional eyepiece on the top or opposite side.

A

dual head

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21
Q

is the diameter of light visible when looking into the eyepiece lens

A

field of view or “FOV”

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22
Q

a type of stand used for low-power microscopes were the arm and body are integral parts and fixed firmly to the Base

A

Fixed arm

23
Q

The method by which the specimen’s distance to the objective lens is adjusted to provide a sharp image.

A

Focus

24
Q
  • upper portion of the microscope, contains the eyepiece tube or tubes and prisms

what are its 4 kinds?

A

Head:

  • Monocular Head
  • Binocular Head
  • Dual Head
    -Trinocular Head
25
Q

some microscopes feature a rotating arm pivot point that can be adjusted to allow for more comfortable viewing

A

inclination joint

26
Q

This adjustment allows the distance between the eyepiece lenses, found on stereo or binocular microscope, to be set either closer or farther apart for more countable viewing.

A

Interpupillary Adjustment

27
Q

A slide holder featuring two knobs that allow the slide position to be adjusted forwards or backwards and from side to side.

A

Mechanical Stage

28
Q

is the metric linear measurement used for microscopy

A

micrometer or micron

29
Q

a microscope head featuring a single eyepiece lens

A

monocular head

30
Q

Important on high-power microscopes, this number is an expression of the lens’ ability to resolve fine detail in the observed object.

A

Numerical Aperture (N.A.)

31
Q

A microscope that is calibrated this way will maintain the specimen in the center of the view when the objective lens is rotated

A

parcentered

32
Q

a microscope that is calibrated this way will maintain the specimen in focus when the objective lens is rotated

A

parfocal

33
Q

Some microscopes feature a ______ that is visible when looking through the eyepiece lens

A

pointer

34
Q

typically used with low-power microscopes, this type of stand features a single post attached to the base allowing the body of the microscope to be rotated or adjusted vertically.

A

post stand

35
Q

a lens system’s ability to resolve fine details of the observed object

A

resolution

36
Q

this special grid pattern is inserted into the eyepiece lens for taking measurements of objects seen through the microscope

A

reticle

37
Q

a separate light connected to the microscope body that produces a ring of light

A

ring light

38
Q

this type of lens improves image quality by presenting a sharper image with less distortion in the perimeter of the field of view

A

Semi-Plan Lenses

39
Q

this mechanical device prevents gear damage to the microscope when the focus adjustment reaches its maximum level

A

slip clutch

40
Q

the mechanical stage allows for adjustment of the slide position forward, backward, left, or right.

A

stage adjustment knobs

41
Q

found on low-power microscopes, this frosted circular glass plate fits in over the lower illuminator.

A

stage plate

42
Q

for low-power microscopes, three types of connections between the microscope body

A

Post Stand,
Fixed Arm,
Universal Stand

43
Q

when using two objectives slightly offset, the image is presented in 3d.

A

stereo

44
Q

this area is located directly below the stage itself

A

sub-stage

45
Q

this type of adapter is useful for attaching still cameras to the microscope

A

T-mount

46
Q

A factory adjustment of the focusing mechanisms that makes microscope easy to focus, but tight enough to prevent the stage from drifting due to the weight of the stage or tube.

A

tension adjustment

47
Q

this type of microscope features two eyepieces for viewing and a third for attaching a camera

A

trinocular head

48
Q

also called a revolving nosepiece, or simply, nosepiece, this structure holds the objective lenses

A

turret

49
Q

a long, boom-type arm used to support a (low-power) microscope body.

A

universal stand

50
Q

these wide-diameter eyepiece lenses offer a broader FOV when viewing a specimen

A

widefield eyepiece lenses

51
Q

in XR, the X represent the magnification of the lens while R stands for ________

A

“retractable”

52
Q

lenses that contain a spring-loaded mechanism that allows them to telescope inward to prevent damage to the lens and slide in case of slide contact

A

XR lenses

53
Q

are brand of specialty paper-based cleaning wipes suited for cleaning lab equipment and instruments, camera lenses, fiber optic connectors and other sensitive electronic components

A

Kimwipes

MCB LAB (4 decks)

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