Aseptic Techniques

Question 1

a set of routine measures taken to prevent cultures, sterile media stocks, and other solutions from being contaminated

Answer 1

Aseptic Techniques by Bykowski et al., 2008

Question 2

a series of steps to prevent contamination during manipulations of cultures and sterile media

Answer 2

Aseptic Techniques by Madigan et al., 2015

Question 3

Aseptic Techniques (15)

Answer 3

• wear appropriate lab PPE
• clean and disinfect floor
• use UV lamp or laminar flow hood
• disinfect workbenches
• appropriate sterilization procedure
• handwashing and disinfection
• handle microbial cultures with caution
• effective sterilization of inoculating loops
• proper dispensing of culture media or solutions
• limit air exposure of cultures and media
• avoid breathing on cultures or sterile instruments
• always work near a flame
• disinfect glass slides and coverslips before washing
• observe proper handling and disposal of contaminated materials
• decontaminate used glassware, culture media, and other materials appropriately

Question 4

CULTURE MEDIA PREPARATION (General Steps) - 6

Answer 4

• Weighing of dehydrated media or media ingredients
• Dissolving of components in the solvent
• Checking/adjustment of pH (optional)
• Dispensing into glassware/containers
• Capping or plugging with cotton
• Sterilization (usually at 15 psi for 15 minutes)

Question 5

BSL meaning

Answer 5

biosafety level

Question 6

lowest safety level

• no containment
• defined organism
• not known to cause disease in adult human
• example: non-pathogenic, E.coli

Answer 6

BSL-1

Question 7

safety level

• is moderate danger if inhale, swallow or expose to skin
• containment
• moderate risk
• disease of varying severity
• example: Influenza, Lyme Disease

Answer 7

BSL-2

Question 8

safety level

• is severe or potentially lethal disease
• indigenous or exotic agents
• high containment
• aerosol transmission
• example: HIV, * flu, tuberculosis

Answer 8

BSL-3

Question 9

highest safety level

• max containment
• dangerous or exotic agents
• life threatening disease
• example: Ebola, Sars-CoV2

Answer 9

BSL-4

Question 10

BSL-3 and BSL-4 autoclave requirements

Answer 10

Pass-thru autoclave with Bioseal required in laboratory room