Virology I & II Flashcards
Name some viral diseases present in humans
In humans there is a wide range of viral diseases e.g. - cancer - immunodeficiency - respiratory these can be acute or chronic
What are the different structures of viral genomes?
ssRNA - single stranded RNA
dsRNA - double stranded RNA
ssDNA - single stranded DNA
dsDNA - double stranded DNA
Double stranded genomes have complementary base pairings
RNA genomes are linear or circular
Genomes can encode info in positive or negative 5’-3’ or 3’-5’ directions
Name some examples of HIV associated pathogens
Virus - KSHV or HSV
Bacteria - mycobacterium & salmonella
Parasites - Toxoplasma gandii
Fungi - Candida
Use the central dogma to explain how virus’ form proteins in the host cell
- DNA replication via DNA polymerase (DNA -> DNA)
- Transcription occurs via RNA Polymerase (DNA ->
mRNA) - Translation of proteins via ribosomes (mRNA -> protein)
What are the 2 types of way a virus can cause disease?
Directly - as a product of their host range & tissue tropism
Indirectly - by promoting/inhibiting cell functions
Which structural proteins are present in viruses?
Capsids formed by proteins have viral nucleic acids packaged into it
Proteins interact with one another in protein-protein interactions
What is the role of polymerases?
Polymerase proteins catalyse nucleotides into nucleic acids
DNA viral genomes produce DNA Polymerases
RNA viral genomes produce RNA Polymerases
Polymerase proteins interact with specific nucleic acids in proteins-nucleic acid
interactions
Outline how viral replication occurs
- Entry into cell
- attachment/entry through glycoprotein on virus with
receptors on host cell via direct fusion or endocytosis - Genome movement within cell where most
advantageous nucleotides found- intracellular structures used to synthesise viral DNA
- Genome replication
- Genome packaging into protein shells encoded by new
viral DNA- packaging sequences in viral DNA/RNA
- Exit from cell
- budding or lysis
How can viruses cause cancer?
As cells divide in cell cycle, nucleotides are produced that can be used by viral polymerases to form new viral genomes
Some viruses can produce proteins ensuring continuous cell cycle - uncontrollable cell division
~ 30% cancer caused by viruses
What is pathogenesis?
When viral and cellular proteins shape the intracellular environment to facilitate viral replication and evasion of the immune response which leads to disease
What is meant by a chronic disease?
A persistent or life-long disease (typically anything > 3 months
Give examples of viral infections and how they’re transmitted
Respiratory viral infections:
- Adenovirus / Influenza
- replicate in lungs
- transmitted by air via coughing, sneezing, breathing etc.
Genital infections:
- replicate in procreation tissues
- transmitted via sexual intercourse
Immunodeficiency Virus’
- replicate in immunity cells
- prevent immune function
- indirect infection
some choose not to replicate immediately and so cannot be detected by the immune system
How do HIV cause T cell death?
Causes apoptosis of immature thymocytes induced by death receptors and activation-induced cell death
HIV inhibits immune cell function resulting in patients being open to attack from any infectious bacteria or virus causing diseases
What is an acute disease?
Disease that are severe and sudden in onset - are typically resolved quickly
How is it beneficial for virus’ to replicate within host cells?
Host cells contain nucleotides that virus’ can use to produce their own viral DNA
Virus’ also use these nucletoides to encode proteins for their protein coat in which the newly synthesised viral DNA is packaged. Protein formation cannot occur outside a cell, therefore replication within host cells is beneficial for this purpose also
