Virology

Question 1

As a general rule, RNA viruses are single-straded and replication in the___ while DNA viruses are double-stranded and replicate in the___.

Answer 1

RNA = cytoplasm
DNA = nucleus

Question 2

What is the best definition of heterophile antibodies as produced in EBV infection?

hint: Ig__ with affinity for what?

Answer 2

IgM antibodies with affinity for beef RBC (sheep and horse RBCs)

Question 3

Which protein is expressed very early in HIV infection and its detection in assays has been used to diagnose seroconversion in HIV?

Answer 3

p24

Question 4

Virus transport medium containing pcn, gent and ampho is used to collect and transport specimens for viral testing because this medium does what?

Answer 4

It inhibits bacterial and fungal growth

Question 5

Which of the following statements about herpesviruses are true except which one:

1. there are eight of them
2. they’re morphologically identical
3. they’re all DNA viruses
4. They all cause a vesicular rash
5. They can all remain latent

Answer 5

4 is false - EBV doesn’t present with a rash

Question 6

Which of the following statements about herpesviruses are true except which one:

1. there are eight of them
2. they’re morphologically identical
3. they’re all DNA viruses
4. They all cause a vesicular rash
5. They can all remain latent

Answer 6

4 is false - EBV doesn’t present with a rash

Question 7

What test is the primary screening test for HIV?

Answer 7

serum enzyme linked immunoassay

Question 8

What virus are cold sores associated with?

Answer 8

HSV1

Question 9

At the end of 40 cycles of PCR, how many DNA molecules are theoretically present in the reaction if one starts with one ds DNA molecule as the template?

Answer 9

2^40 (because there were two strands)