Viro exam 2 Flashcards
Polymerase Chain Reaction (PCR) is used to?
a. Stain the virus and make it visible
b. Detect any viral proteins
c. Amplify the viral DNA
d. Detect antibodies
c. Amplify the viral DNA
Which is not a step of PCR?
a. Annealing
b. Processing
c. Extension
d. Denaturing
b. Processing
True or False?
Metagenomics is used to detect specific pathogens in a sample?
False- used for random detection
Which diagnostic test allows hundreds of pathogens to be screened at the same time using one chip?
a. PCR
b. Metagenomics
c. Microarrays
d. ELISA
c. Microarrays
Acyclovir is used for the treatment of?
a. Herpesviruses
b. HIV
c. Poxviruses
d. Flu viruses
a. Herpesviruses
Which is not true about Acyclovir?
a. Administered as a prodrug
b. Synthetic form of Guanosine
c. Prevents elongation of NA sequence
d. Toxic to the host cell
d. Toxic to the host cell
Amantadine is used to treat the flu virus and works on the ____?
a. M1 channel
b. M2 channel
c. P53
d. Rb
b. M2 channel
Which pair is incorrect?
a. Oseltamivir—> influenza A & B
b. Zidovudine(ZVD)/ Azidothymidine (AZT)—> thymine copycat
c. Zidovudine(ZVD)/ Azidothymidine (AZT)—> Nucleoside Analog reverse transcriptase inhibitors k
d. Protease inhibitors—> HIV
e. All are correct
e. All are correct
Which is not a form of a “live-attenuated” vaccine?
a. Attenuation of viruses by serial passage in cultured cells
b. Attenuation of viruses by serial passage in heterologous hosts
c. Attenuation by selection of cold-adapted mutants/reassortments
d. Using inactivated/non-replicating viruses as vaccines
d. Using inactivated/non-replicating viruses as vaccines
What type of vaccine would you use if you wanted to later test to see who was vaccinated or naturally infected?
a. Live attenuated
b. DIVA
c. Inactivated vaccines
d. Vaccines made from only viral proteins
b. DIVA
True or False
Sterilization destroys/eliminates all forms of microbial life/pathogens
True
Which type of Poxviruses are ovoid and resemble a ball of yarn?
a. Parapoxviruses
b. Orthopoxviruses
c. Suipoxviruses
d. None of the above
a. Parapoxviruses
True or False?
Only the extracellular enveloped visions of poxviruses are infectious
False. both EEV and IMV forms are infectious
What is the correct order of the Poxvirus “Symphony”?
a. Ma-pu-pa-ve-sca
b. Pu-ma-ve-sca
c. Ma-pa-ve-pu-sca
c. Ma-pa-ve-pu-sca
Which is false?
a. Poxviruses are resistant to detergents/disinfectants(low lipid)
b. Poxviruses replicate in the cytoplasm
c. Main species of concern in Cowpox are cows
d. Sheep show a systemic form of the disease in sheep
c. Main species of concern in Cowpox are cows
Which poxvirus shows a classical “horseshoe” shaped area of scabs?
a. Suipoxvirus
b. Cowpox
c. Capripox
d. Pseudocowpox
d. Pseudocowpox
True or False?
Both sheep pox and goat pox are reportable?
True
Which form of fowlpox is considered the most severe?
a. Cutaneous(dry)
b. Diphtheric(wet)
c. Ocular
d. All equally severe
b. Diphtheric(wet)
Which poxvirus will you see Bollinger and barrel bodies?
a. Cowpox
b. Suipox
c. Fowlpox
d. Capripox
c. Fowlpox
True or false?
Circoviruses can replicate in any cell
False. can only replicate in an actively dividing cell
Which virus causes PMWS in piglets?
a. Porcine circovirus 1
b. Porcine circovirus 2
c. Porcine circovirus 3
d. All porcine circoviruses
b. Porcine circovirus 2
What type of inclusion bodies are seen in circovirus infections?
a. Bollinger bodies
b. Borrel bodies
c. Cowdry Bodies
d. Botyroid Bodies
d. Botyroid Bodies
Porcine dermatitis and nephropathy syndrome is caused by?
a. Porcine circovirus 1
b. Porcine circovirus 2
c. Porcine Herpesvirus 1
d. Porcine Herpesvirus 2
b. Porcine circovirus 2
What genus is the chicken anemia virus in?
a. Gyrovirus
b. Circovirus
a. Gyrovirus