Vibrionaceae Flashcards
identify
- gram stain reaction (-)
- oxidase activity (+)
- O/129 Suscpetibility 150 μg (R)
- growth on TCBS agar (-)
- 0% NaCl (+)
- 6.5% NaCl (-)
- glucose (+)
- inositol (-)
- mannitol (+/-)
- sucrose (+/-)
- gelatin liquefaction (+)
identification of Vibrio, Aeromonas, and Plesiomonas
Aeromonas
identify
- Acute, secretory diarrhea often accompanied by vomiting
- An acute, dysenteric form of diarrhea (similar to shigellosis), with blood and mucus
- Chronic diarrhea usually lasts more than 10 days
- Cholera-like disease, including rice water stools
- The nebulous syndrome commonly referred to as traveler’s diarrhea (like enterotoxigenic E. coli)
Aeromonas
identify
V. cholerae O1 organisms are divided into three (3) serotypes
- Ogawa (A, B)
- Inaba (A, C)
- Hikojima (A. B, C)
identify
(?) can enhance the isolation of plesiomonads
Plesiomonas colonies are (?) in this medium, and most coliform colonies are (?)
- Inositol brilliant green bile agar
- white to pink
- green or pink
identify
- gastroenteritis
- food poisonong (foodborne outbreaks)
- “kanagawa phenomenon”
Vibrio paraheamolyticus
identify
(?) is known as cholera toxin, or choleragen
Enterotoxin
identify
Stool specimens suspected of containing Vibrio spp. should be collected and transported in (?)
Cary Blair medium
identify
It also grows well on TCBS, where it yields green colonies
Vibrio parahaemolyticus
identify
found in
- freshwater, eustarine, and marine environments
- animal meat products
- recognized as “enteric pathogens”
Aeromonas
identify
- Primary septicemia and wound infections
- organ failure
Vibrio vulnifucus
choose the correct answer
- V. mimicus
- V. parahaemolyticus
- V. damsela
- V. vulnificus strains (most)
a. non-sucrose fermenting species
b. sucrose-fermenting species
vibrio
a. non-sucrose fermenting species
(green colonies)
identify
three (3) major subgroups of V. cholerae
- V. cholerae O1
- V. cholerae O139
- V. cholerae non-O1
identify
For separation of aeromonads from plesiomonads, one can use the fermentation of inositol, in which aeromonads are (?) and plesiomonads are (?)
- negative
- positive
identify
Aeromonas spp. cells are (?) rather than curved
straight
identify
- growth in 0% NaCl (-)
- growth in 6% NaCl (+/-)
- voges-proskauer (-)
- lysine (+)
- lactose (+)
- sucrose (-)
vibrio
V. vulnificus
identify
- growth in 0% NaCl (-)
- growth in 6% NaCl (+)
- voges-proskauer (+)
- lysine (+)
- lactose (-)
- sucrose (+)
vibrio
V. alginolyticus
identify
gastroenteritis, most common in children
an emerging anteric pathogen (foodborne and waterborne illness)
P. shigelloides
identify
it is the only species in the genus Plesiomonas
P. shigelloides
identify
Either (?) or (?) colonies are produced, depending on whether they can ferment sucrose
vibrio
- yellow
- green
identify
- oxidase (+)
- glucose (+)
- facultatively anaerobic
- gram-negative bacilli
Plesiomonas
identify
- growth in 0% NaCl (+)
- growth in 6% NaCl (+/-)
- voges-proskauer (+/-)
- lysine (+)
- lactose (-)
- sucrose (+)
vibrio
V. cholerae
choose the correct answer
non-sucrose fermenting
a. green colonies
b. yellow colonies
vibrio
a. green colonies
identify
A more invasive, dysenteric form that resembles colitis
Plesiomonas
identify
- gram stain reaction (-)
- oxidase activity (+)
- O/129 Suscpetibility 150 μg (S)
- growth on TCBS agar (-)
- 0% NaCl (+)
- 6.5% NaCl (-)
- glucose (+)
- inositol (+)
- mannitol (-)
- sucrose (-)
- gelatin liquefaction (-)
identification of Vibrio, Aeromonas, and Plesiomonas
Plesiomonas
identify
(?) agar is recommended for the culture of Vibrio spp.
Thiosulfate citrate bile salt sucrose (TCBS)
identify
profuse, watery diarrhea leading to dehydration, hypotension, and often death
occurs in epidemics and pandemics that span the globe
Cholera
identify
(?) and (?) are the only Vibrio spp. that do not require salt for growth
- V. cholerae
- V. mimicus
identify
(?) and (?) Ph
indicators are added to the TCBS medium
vibrio
- Bromothymol
- thymol blue
identify
a key test for distinguishing the halophilic species from V. cholerae, V. mimicus, and Aeromonas spp. is growth in (?) with (?)
- nutrient broth
- 6% salt
identify
Freshwater inhabitant that is transmitted to humans by ingestion of contaminated water or by exposure of disrupted skin and mucosal surfaces
P. shigelloides
identify
- gram stain reaction (-)
- oxidase activity (+)
- O/129 Suscpetibility 150 μg (S)
- growth on TCBS agar (+)
- 0% NaCl (-/+)
- 6.5% NaCl (+)
- glucose (+)
- inositol (-)
- mannitol (+)
- sucrose (+/-)
- gelatin liquefaction (+)
identification of Vibrio, Aeromonas, and Plesiomonas
Vibrio
identify
DNase positive
Aeromonas spp.
identify
produces a hemolysin, gives positive results on the Voges-Proskauer test, and is resistant to polymyxin B, agglutinate chicken red blood cells
V. cholerae
El Tor biotype
identify
V. cholerae toxin can be detected in stool using an (?) or a commercially available (?)
- enzyme-linked immunosorbent assay (ELISA)
- latex agglutination test
identify
DNase negative
Plesiomonas
identify
two (2) biotypes of epidemic V. cholerae
- Classic
- El Tor
identify
(?) is the species most frequently associated with gastrointestinal infections, especially in neonate and pediatric populations
it has been associated with inflammatory bowel disease
Aeromonas
A. caviae
identify
Serotyping should be performed immediately to further characterize (?)
V. cholerae isolates
identify
A vibrostatic test using (?) has been used to separate Vibrios from other oxidase-positive glucose fermenters and to differentiate V. cholerae O1 and non- O1 from other Vibrio spp.
0129 impregnated disks
identify
- gram-negative straight rods with rounded ends or coccobacillary facultative anaerobes
- oxidase (+)
- catalase (+)
- indole (+)
- beta hemolytic (most)
Aeromonas
identify
- found in soil and aquatic environments
- diarrheal disease
- cause of at least three (3) major clinical types of gastroenteritis
- more common watery or secretory diarrhea
- A subacute or chronic disease that lasts from 14 days to 2 to 3 months
Plesiomonas
identify
- oxidase (+)
- 0129 vibriostatic compound sesitivity (+)
- polar flagella (+)
Vibrio spp.
identify
rapid darting or shooting-star motility
Vibrio cholerae
identify
- facultatively anaerobic
- catalase (+)
- oxidase (+)
- can reduce nitrate to nitrite
- inositol (-)
Vibrio spp.
identify
Plesiomonas is susceptible to (?)
ampicillin
identify
- catalase (-)
- oxidase (-)
- cannot reduce nitrate to nitrite
vibrio
V. metschnikovii
choose the correct answer
sucrose-fermenting
a. yellow colonies
b. green colonies
vibrio
a. yellow colonies
identify
Least pathogenic for humans
Vibrio alginolyticus
identify
found in aquatic environments;
- freshwater
- brackish or estuarine water
- marine or salt water
Vibrio
choose the correct answer
- V. cholerae
- V. alginolyticus
- V. fluvialis
- V. furnissii
- V. cincinnatiensis
- V. metschnikovii
- V. vulnificus strains (some)
a. sucrose-fermenting species
b. non-sucrose fermenting species
vibrio
a. sucrose-fermenting species
(yellow colonies)
identify
- growth in 0% NaCl (-)
- growth in 6% NaCl (+)
- voges-proskauer (-)
- lysine (+)
- lactose (-)
- sucrose (-)
vibrio
V. parahaemolyticus
identify
nonhemolytic Aeromonas spp.
Aeromonas caviae
identify
(?) and (?) grow well in nutrient broth with 0% NaCl, but not in 6% NaCl
- Aeromonads
- Plesiomonads
