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BACTE | MIDTERMS > Vibrionaceae > Flashcards

Vibrionaceae Flashcards

1
Q

identify

  • gram stain reaction (-)
  • oxidase activity (+)
  • O/129 Suscpetibility 150 μg (R)
  • growth on TCBS agar (-)
  • 0% NaCl (+)
  • 6.5% NaCl (-)
  • glucose (+)
  • inositol (-)
  • mannitol (+/-)
  • sucrose (+/-)
  • gelatin liquefaction (+)

identification of Vibrio, Aeromonas, and Plesiomonas

A

Aeromonas

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2
Q

identify

  • Acute, secretory diarrhea often accompanied by vomiting
  • An acute, dysenteric form of diarrhea (similar to shigellosis), with blood and mucus
  • Chronic diarrhea usually lasts more than 10 days
  • Cholera-like disease, including rice water stools
  • The nebulous syndrome commonly referred to as traveler’s diarrhea (like enterotoxigenic E. coli)
A

Aeromonas

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3
Q

identify

V. cholerae O1 organisms are divided into three (3) serotypes

A
  • Ogawa (A, B)
  • Inaba (A, C)
  • Hikojima (A. B, C)
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4
Q

identify

(?) can enhance the isolation of plesiomonads

Plesiomonas colonies are (?) in this medium, and most coliform colonies are (?)

A
  • Inositol brilliant green bile agar
  • white to pink
  • green or pink
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5
Q

identify

  • gastroenteritis
  • food poisonong (foodborne outbreaks)
  • “kanagawa phenomenon”
A

Vibrio paraheamolyticus

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6
Q

identify

(?) is known as cholera toxin, or choleragen

A

Enterotoxin

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7
Q

identify

Stool specimens suspected of containing Vibrio spp. should be collected and transported in (?)

A

Cary Blair medium

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8
Q

identify

It also grows well on TCBS, where it yields green colonies

A

Vibrio parahaemolyticus

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9
Q

identify

found in

  • freshwater, eustarine, and marine environments
  • animal meat products
  • recognized as “enteric pathogens”
A

Aeromonas

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10
Q

identify

  • Primary septicemia and wound infections
  • organ failure
A

Vibrio vulnifucus

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11
Q

choose the correct answer

  • V. mimicus
  • V. parahaemolyticus
  • V. damsela
  • V. vulnificus strains (most)

a. non-sucrose fermenting species
b. sucrose-fermenting species

vibrio

A

a. non-sucrose fermenting species
(green colonies)

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12
Q

identify

three (3) major subgroups of V. cholerae

A
  • V. cholerae O1
  • V. cholerae O139
  • V. cholerae non-O1
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13
Q

identify

For separation of aeromonads from plesiomonads, one can use the fermentation of inositol, in which aeromonads are (?) and plesiomonads are (?)

A
  • negative
  • positive
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14
Q

identify

Aeromonas spp. cells are (?) rather than curved

A

straight

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15
Q

identify

  • growth in 0% NaCl (-)
  • growth in 6% NaCl (+/-)
  • voges-proskauer (-)
  • lysine (+)
  • lactose (+)
  • sucrose (-)

vibrio

A

V. vulnificus

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16
Q

identify

  • growth in 0% NaCl (-)
  • growth in 6% NaCl (+)
  • voges-proskauer (+)
  • lysine (+)
  • lactose (-)
  • sucrose (+)

vibrio

A

V. alginolyticus

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17
Q

identify

gastroenteritis, most common in children

an emerging anteric pathogen (foodborne and waterborne illness)

A

P. shigelloides

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18
Q

identify

it is the only species in the genus Plesiomonas

A

P. shigelloides

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19
Q

identify

Either (?) or (?) colonies are produced, depending on whether they can ferment sucrose

vibrio

A
  • yellow
  • green
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20
Q

identify

  • oxidase (+)
  • glucose (+)
  • facultatively anaerobic
  • gram-negative bacilli
A

Plesiomonas

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21
Q

identify

  • growth in 0% NaCl (+)
  • growth in 6% NaCl (+/-)
  • voges-proskauer (+/-)
  • lysine (+)
  • lactose (-)
  • sucrose (+)

vibrio

A

V. cholerae

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22
Q

choose the correct answer

non-sucrose fermenting

a. green colonies
b. yellow colonies

vibrio

A

a. green colonies

23
Q
A
24
Q

identify

A more invasive, dysenteric form that resembles colitis

A

Plesiomonas

25
Q

identify

  • gram stain reaction (-)
  • oxidase activity (+)
  • O/129 Suscpetibility 150 μg (S)
  • growth on TCBS agar (-)
  • 0% NaCl (+)
  • 6.5% NaCl (-)
  • glucose (+)
  • inositol (+)
  • mannitol (-)
  • sucrose (-)
  • gelatin liquefaction (-)

identification of Vibrio, Aeromonas, and Plesiomonas

A

Plesiomonas

26
Q

identify

(?) agar is recommended for the culture of Vibrio spp.

A

Thiosulfate citrate bile salt sucrose (TCBS)

27
Q

identify

profuse, watery diarrhea leading to dehydration, hypotension, and often death

occurs in epidemics and pandemics that span the globe

A

Cholera

28
Q

identify

(?) and (?) are the only Vibrio spp. that do not require salt for growth

A
  • V. cholerae
  • V. mimicus
29
Q

identify

(?) and (?) Ph
indicators are added to the TCBS medium

vibrio

A
  • Bromothymol
  • thymol blue
30
Q

identify

a key test for distinguishing the halophilic species from V. cholerae, V. mimicus, and Aeromonas spp. is growth in (?) with (?)

A
  • nutrient broth
  • 6% salt
31
Q

identify

Freshwater inhabitant that is transmitted to humans by ingestion of contaminated water or by exposure of disrupted skin and mucosal surfaces

A

P. shigelloides

32
Q

identify

  • gram stain reaction (-)
  • oxidase activity (+)
  • O/129 Suscpetibility 150 μg (S)
  • growth on TCBS agar (+)
  • 0% NaCl (-/+)
  • 6.5% NaCl (+)
  • glucose (+)
  • inositol (-)
  • mannitol (+)
  • sucrose (+/-)
  • gelatin liquefaction (+)

identification of Vibrio, Aeromonas, and Plesiomonas

A

Vibrio

33
Q

identify

DNase positive

A

Aeromonas spp.

34
Q

identify

produces a hemolysin, gives positive results on the Voges-Proskauer test, and is resistant to polymyxin B, agglutinate chicken red blood cells

V. cholerae

A

El Tor biotype

35
Q

identify

V. cholerae toxin can be detected in stool using an (?) or a commercially available (?)

A
  • enzyme-linked immunosorbent assay (ELISA)
  • latex agglutination test
36
Q

identify

DNase negative

A

Plesiomonas

37
Q

identify

two (2) biotypes of epidemic V. cholerae

A
  • Classic
  • El Tor
38
Q

identify

(?) is the species most frequently associated with gastrointestinal infections, especially in neonate and pediatric populations

it has been associated with inflammatory bowel disease

Aeromonas

A

A. caviae

39
Q

identify

Serotyping should be performed immediately to further characterize (?)

A

V. cholerae isolates

40
Q

identify

A vibrostatic test using (?) has been used to separate Vibrios from other oxidase-positive glucose fermenters and to differentiate V. cholerae O1 and non- O1 from other Vibrio spp.

A

0129 impregnated disks

41
Q

identify

  • gram-negative straight rods with rounded ends or coccobacillary facultative anaerobes
  • oxidase (+)
  • catalase (+)
  • indole (+)
  • beta hemolytic (most)
A

Aeromonas

42
Q

identify

  • found in soil and aquatic environments
  • diarrheal disease
  • cause of at least three (3) major clinical types of gastroenteritis
  • more common watery or secretory diarrhea
  • A subacute or chronic disease that lasts from 14 days to 2 to 3 months
A

Plesiomonas

43
Q

identify

  • oxidase (+)
  • 0129 vibriostatic compound sesitivity (+)
  • polar flagella (+)
A

Vibrio spp.

44
Q

identify

rapid darting or shooting-star motility

A

Vibrio cholerae

45
Q

identify

  • facultatively anaerobic
  • catalase (+)
  • oxidase (+)
  • can reduce nitrate to nitrite
  • inositol (-)
A

Vibrio spp.

46
Q

identify

Plesiomonas is susceptible to (?)

A

ampicillin

47
Q

identify

  • catalase (-)
  • oxidase (-)
  • cannot reduce nitrate to nitrite

vibrio

A

V. metschnikovii

48
Q

choose the correct answer

sucrose-fermenting

a. yellow colonies
b. green colonies

vibrio

A

a. yellow colonies

49
Q

identify

Least pathogenic for humans

A

Vibrio alginolyticus

50
Q

identify

found in aquatic environments;

  • freshwater
  • brackish or estuarine water
  • marine or salt water
A

Vibrio

51
Q

choose the correct answer

  • V. cholerae
  • V. alginolyticus
  • V. fluvialis
  • V. furnissii
  • V. cincinnatiensis
  • V. metschnikovii
  • V. vulnificus strains (some)

a. sucrose-fermenting species
b. non-sucrose fermenting species

vibrio

A

a. sucrose-fermenting species
(yellow colonies)

52
Q

identify

  • growth in 0% NaCl (-)
  • growth in 6% NaCl (+)
  • voges-proskauer (-)
  • lysine (+)
  • lactose (-)
  • sucrose (-)

vibrio

A

V. parahaemolyticus

53
Q

identify

nonhemolytic Aeromonas spp.

A

Aeromonas caviae

54
Q

identify

(?) and (?) grow well in nutrient broth with 0% NaCl, but not in 6% NaCl

A
  • Aeromonads
  • Plesiomonads

BACTE | MIDTERMS (3 decks)

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