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BACTE | MIDTERMS > Vibrionaceae > Flashcards

Vibrionaceae Flashcards

1
Q

identify

  • gram stain reaction (-)
  • oxidase activity (+)
  • O/129 Suscpetibility 150 μg (R)
  • growth on TCBS agar (-)
  • 0% NaCl (+)
  • 6.5% NaCl (-)
  • glucose (+)
  • inositol (-)
  • mannitol (+/-)
  • sucrose (+/-)
  • gelatin liquefaction (+)

identification of Vibrio, Aeromonas, and Plesiomonas

A

Aeromonas

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2
Q

identify

  • Acute, secretory diarrhea often accompanied by vomiting
  • An acute, dysenteric form of diarrhea (similar to shigellosis), with blood and mucus
  • Chronic diarrhea usually lasts more than 10 days
  • Cholera-like disease, including rice water stools
  • The nebulous syndrome commonly referred to as traveler’s diarrhea (like enterotoxigenic E. coli)
A

Aeromonas

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3
Q

identify

V. cholerae O1 organisms are divided into three (3) serotypes

A
  • Ogawa (A, B)
  • Inaba (A, C)
  • Hikojima (A. B, C)
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4
Q

identify

(?) can enhance the isolation of plesiomonads

Plesiomonas colonies are (?) in this medium, and most coliform colonies are (?)

A
  • Inositol brilliant green bile agar
  • white to pink
  • green or pink
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5
Q

identify

  • gastroenteritis
  • food poisonong (foodborne outbreaks)
  • “kanagawa phenomenon”
A

Vibrio paraheamolyticus

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6
Q

identify

(?) is known as cholera toxin, or choleragen

A

Enterotoxin

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7
Q

identify

Stool specimens suspected of containing Vibrio spp. should be collected and transported in (?)

A

Cary Blair medium

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8
Q

identify

It also grows well on TCBS, where it yields green colonies

A

Vibrio parahaemolyticus

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9
Q

identify

found in

  • freshwater, eustarine, and marine environments
  • animal meat products
  • recognized as “enteric pathogens”
A

Aeromonas

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10
Q

identify

  • Primary septicemia and wound infections
  • organ failure
A

Vibrio vulnifucus

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11
Q

choose the correct answer

  • V. mimicus
  • V. parahaemolyticus
  • V. damsela
  • V. vulnificus strains (most)

a. non-sucrose fermenting species
b. sucrose-fermenting species

vibrio

A

a. non-sucrose fermenting species
(green colonies)

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12
Q

identify

three (3) major subgroups of V. cholerae

A
  • V. cholerae O1
  • V. cholerae O139
  • V. cholerae non-O1
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13
Q

identify

For separation of aeromonads from plesiomonads, one can use the fermentation of inositol, in which aeromonads are (?) and plesiomonads are (?)

A
  • negative
  • positive
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14
Q

identify

Aeromonas spp. cells are (?) rather than curved

A

straight

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15
Q

identify

  • growth in 0% NaCl (-)
  • growth in 6% NaCl (+/-)
  • voges-proskauer (-)
  • lysine (+)
  • lactose (+)
  • sucrose (-)

vibrio

A

V. vulnificus

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16
Q

identify

  • growth in 0% NaCl (-)
  • growth in 6% NaCl (+)
  • voges-proskauer (+)
  • lysine (+)
  • lactose (-)
  • sucrose (+)

vibrio

A

V. alginolyticus

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17
Q

identify

gastroenteritis, most common in children

an emerging anteric pathogen (foodborne and waterborne illness)

A

P. shigelloides

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18
Q

identify

it is the only species in the genus Plesiomonas

A

P. shigelloides

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19
Q

identify

Either (?) or (?) colonies are produced, depending on whether they can ferment sucrose

vibrio

A
  • yellow
  • green
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20
Q

identify

  • oxidase (+)
  • glucose (+)
  • facultatively anaerobic
  • gram-negative bacilli
A

Plesiomonas

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21
Q

identify

  • growth in 0% NaCl (+)
  • growth in 6% NaCl (+/-)
  • voges-proskauer (+/-)
  • lysine (+)
  • lactose (-)
  • sucrose (+)

vibrio

A

V. cholerae

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22
Q

choose the correct answer

non-sucrose fermenting

a. green colonies
b. yellow colonies

vibrio

A

a. green colonies

24
Q

identify

A more invasive, dysenteric form that resembles colitis

A

Plesiomonas

25
# identify * **gram stain reaction** (-) * **oxidase activity** (+) * **O/129 Suscpetibility 150 μg** (S) * **growth on TCBS agar** (-) * **0% NaCl** (+) * **6.5% NaCl** (-) * **glucose** (+) * **inositol** (+) * **mannitol** (-) * **sucrose** (-) * **gelatin liquefaction** (-) | identification of Vibrio, Aeromonas, and Plesiomonas
Plesiomonas
26
# identify **(?)** agar is recommended for the culture of Vibrio spp.
Thiosulfate citrate bile salt sucrose (TCBS)
27
# identify profuse, watery diarrhea leading to dehydration, hypotension, and often death occurs in epidemics and pandemics that span the globe
Cholera
28
# identify **(?)** and **(?)** are the only Vibrio spp. that do not require salt for growth
* V. cholerae * V. mimicus
29
# identify **(?)** and **(?)** Ph indicators are **added** to the TCBS medium | vibrio
* Bromothymol * thymol blue
30
# identify a key test for distinguishing the halophilic species from V. cholerae, V. mimicus, and Aeromonas spp. is growth in **(?)** with **(?)**
* nutrient broth * 6% salt
31
# identify Freshwater inhabitant that is transmitted to humans by ingestion of contaminated water or by exposure of disrupted skin and mucosal surfaces
P. shigelloides
32
# identify * **gram stain reaction** (-) * **oxidase activity** (+) * **O/129 Suscpetibility 150 μg** (S) * **growth on TCBS agar** (+) * **0% NaCl** (-/+) * **6.5% NaCl** (+) * **glucose** (+) * **inositol** (-) * **mannitol** (+) * **sucrose** (+/-) * **gelatin liquefaction** (+) | identification of Vibrio, Aeromonas, and Plesiomonas
Vibrio
33
# identify DNase positive
Aeromonas spp.
34
# identify produces a hemolysin, gives positive results on the Voges-Proskauer test, and is resistant to polymyxin B, agglutinate chicken red blood cells | V. cholerae
El Tor biotype
35
# identify V. cholerae toxin can be detected in stool using an **(?)** or a commercially available **(?)**
* enzyme-linked immunosorbent assay (ELISA) * latex agglutination test
36
# identify DNase negative
Plesiomonas
37
# identify two (2) biotypes of epidemic V. cholerae
* Classic * El Tor
38
# identify **(?)** is the species most frequently associated with **gastrointestinal infections**, especially in **neonate** and **pediatric** populations it has been associated with **inflammatory bowel disease** | Aeromonas
A. caviae
39
# identify Serotyping should be performed immediately to further characterize **(?)**
V. cholerae isolates
40
# identify A **vibrostatic test** using **(?)** has been used to separate Vibrios from other oxidase-positive glucose fermenters and to differentiate V. cholerae O1 and non- O1 from other Vibrio spp.
0129 impregnated disks
41
# identify * gram-negative straight rods with rounded ends or coccobacillary facultative anaerobes * oxidase (+) * catalase (+) * indole (+) * beta hemolytic (most)
Aeromonas
42
# identify * found in **soil** and **aquatic** environments * diarrheal disease * **cause** of at least three (3) major clinical types of gastroenteritis * more common **watery** or **secretory** diarrhea * A **subacute** or **chronic disease** that lasts from 14 days to 2 to 3 months
Plesiomonas
43
# identify * **oxidase** (+) * **0129 vibriostatic compound sesitivity** (+) * **polar flagella** (+)
Vibrio spp.
44
# identify rapid darting or shooting-star motility
Vibrio cholerae
45
# identify * facultatively anaerobic * catalase (+) * oxidase (+) * can reduce nitrate to nitrite * inositol (-)
Vibrio spp.
46
# identify Plesiomonas is susceptible to **(?)**
ampicillin
47
# identify * catalase (-) * oxidase (-) * cannot reduce nitrate to nitrite | vibrio
V. metschnikovii
48
# choose the correct answer **sucrose-fermenting** a. yellow colonies b. green colonies | vibrio
a. yellow colonies
49
# identify Least pathogenic for humans
Vibrio alginolyticus
50
# identify found in **aquatic environments**; * freshwater * brackish or estuarine water * marine or salt water
Vibrio
51
# choose the correct answer * V. cholerae * V. alginolyticus * V. fluvialis * V. furnissii * V. cincinnatiensis * V. metschnikovii * V. vulnificus strains (some) a. sucrose-fermenting species b. non-sucrose fermenting species | vibrio
a. sucrose-fermenting species (yellow colonies)
52
# identify * **growth in 0% NaCl** (-) * **growth in 6% NaCl** (+) * **voges-proskauer** (-) * **lysine** (+) * **lactose** (-) * **sucrose** (-) | vibrio
V. parahaemolyticus
53
# identify nonhemolytic Aeromonas spp.
Aeromonas caviae
54
# identify **(?)** and **(?)** grow well in nutrient broth with 0% NaCl, but not in 6% NaCl
* Aeromonads * Plesiomonads

BACTE | MIDTERMS (3 decks)

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