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BACTE LESSON 20 > VIBRIO and AEROMONAS > Flashcards

VIBRIO and AEROMONAS Flashcards

1
Q

T or F
Vibrio, Aeromonas,Plesiomonas, Campylobacter are gram (+) cocci.

A

False
Gram(-) bacilli

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2
Q

T or F
Vibrio, Aeromonas, Plesiomonas, Campylobacter are oxidase (+)

A

True

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3
Q

All Bacteria spp. that are commonly found in aquatic environments except:
A. Vibrio
B. Aeromonas
C. Plesiomonas
D. Campylobacter

A

D

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4
Q

Different significant Vibrio spp.

A

V. cholerae
V. parahaemolyticus
V. vulnificus
V. alginolyticus

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5
Q

Most important and common sp. Of Vibrio.

A

Vibrio cholerae

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6
Q

All are factors of Vibrio infection except:
A. Recent consumption of raw seafood (oyster)
B. Eating aquatic plants
C. Exposure of aquatic environment
D. Accidental trauma during contact eith fresh estuarine/marine water
E. Recent immigration/foreign travel

A

B.

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7
Q

Characteristics of Vibrio infection.

A

Gastroenteritis with cholera-like or
Rice water stool

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8
Q

Characteristics of Vibrio under microscope

A
  • Gram (-)
  • Asporogenous
  • Curved
  • comma shaped bacteria
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9
Q

Characteristic of Vibrio in broth media

A

Possess polar, sheathed flagella

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10
Q

Appearance of Vibrioin solid media

A

Peritrichous, unsheathed flagella

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11
Q

Vibrio spp. are susceptible to what compound? And it is used for confirmation.

A

Vibrio static compound O/129 (150ųg)

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12
Q

Mucoid “stringing” rxn. W/ 0.5% sodium deoxycholate.

A

Positive string test

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13
Q

All Vibrio spp. are halophilic except:

A

V. cholerae
V. mimicus
(They are only found in fresh water)

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14
Q

Major subgroups of V. cholerae.

A

V. cholerae O1
V. cholerae O139
V. cholerae non-O1

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15
Q

Subgroup of V. cholerae that is the most common and cause diarrhea infection.

A

V. cholerae O1

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16
Q

Serotypes of V. cholerae O1

A

Ogawa (A,B)
Inaba (A,C)
Hikojima (A,B,C)

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17
Q

Common cause of Epidemic cholera

A

V. cholerae O1
V. cholerae O139

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18
Q

Causative agent of cholera, specifically Asiatic cholera and Epidemic cholera.

A

Vibrio cholerae

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19
Q

Acute diarrheal disease brought by V. cholerae spread through ___.

A

Contaminated water

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20
Q

Watery with numerous flecks of mucus ;10-30 per day

A

Rice-water stool

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21
Q

What do you call the enterotoxin of V. cholerae?

A

Cholera toxin /choleragen

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22
Q

The enterotoxin of V. cholerae stimulates production of ___.

A

Adenylate cyclase

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23
Q

T or F
V. cholerae enterotoxin can cause hypersecretion of electrolytes & water out of the cell.

A

True

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24
Q

Treatment for V. cholerae.

A

IV/oral fluids

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25
Q

V. cholerae is dangerous because it can leads to __.

A

Dehydration

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26
Q

T or F
V. cholerae infection is self-limiting.

A

True

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27
Q

Action of V. cholerae leading to toxin is brought by ___.

A

2 fragments
Fragment A and B

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28
Q

This fragment activates the enzyme adenylate cyclase (cAMP)

A

Fragment A

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29
Q

It facilitates the entry of Fragment A.

A

Fragment B

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30
Q

How does the hypersecretion of electrolytes and water happens?

A

Due to the accumulation of cAMP

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31
Q

Two biogroups of epidemic V. cholerae O1 strains.

A

Classic
El Tor

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32
Q

Predominant biogroup of Epidemic V. cholerae O1 strain.

A

El Tor

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33
Q

All are characteristics of El Tor biogroup of V. cholerae O1 except:
A. VP(+)
B. Non hemolytic
C. Inhibit by polymyxin B
D. Agglutinates chicken RBCs

A

B. It hemolyzes RBCs

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34
Q

All are characteristics of classic biogroup of V. cholerae O1 strain except:
A. Agglutinates chicken RBCs
B. VP (-)
C. Resistant to (polymyxin B)
D. Non hemolytic RBCs

A

A. It doesn’t agglutinates

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35
Q

The second most common Vibrio spp. That cause gastroenteritis.

A

V. parahaemolyticus

36
Q

No. 1 cause of summer diarrhea in Japan.

A

V. parahaemolyticus

37
Q

This Vibrio is thermophiles

A

V. parahaemolyticus

38
Q

Virulence is associated with the production of heat-stable hemolysin.

A

Kanagawa phenomenon

39
Q

In Kanagawa phenomenon, it lyses human RBCs in what agar ?

A

Wagatsuma agar (BAP)

40
Q

High-salt mannitol medium

A

Wagatsuma agar

41
Q

T or F
V. parahaemolyticus is (+) in kanagawa toxin

A

True

42
Q

Lactose positive Vibrio (pink in MAC)

A

V. vulnificus

43
Q

The second most serious type of Vibrio is associated with infections.

A

V. vulnificus

44
Q

Two categories of infections cause by V. vulnificus.

A

Primary septicemia
Wound infection

45
Q

Infection of V. vulnificus brought by consumption of shellfish.

A

Primary septicemia

46
Q

Infection of V. vulnificus brought by traumatic aquatic wounds.

A

Wound infection

47
Q

T or F
The most common infection of V. vulnificus is gastroenteritis.

A

False

48
Q

The least pathogenic and most infrequently isolates sp. of Vibrio.

A

V. alginolyticus

49
Q

V. alginolyticus is strictly halophile, and what % of NaCl?

A

1%

50
Q

V. alginolyticus is prone to what type of occupation?

A

Fishermen and sailors

51
Q

What transport medium is not recommended in Vibrio spp.?

A

Buffered glycerol saline

52
Q

T or F
Vibrio spp. Are fastidious bacteria

A

False-non fastidious

53
Q

Culture medium for Vibrio should contain at least ___.

A

O.5% salt

54
Q

All are the characteristics of Vibrio in SBA/CHOC media except:
A. Medium-large volonies
B. Opaque
C. Translucent
D. Irridescent with greenish hue
E. Smooth

A

C

55
Q

T or F
In MAC, all vibrio spp. are non!Lactose-fermenter except V. cholerae.

A

False- V. vulnificus

56
Q

Most recommended selective medium for Vibrio spp.

A

Thiosulfate Citrate Bile salt Sucrose Agar (TCBS)

57
Q

This medium differentiates the sucrose fermenter and non sucrose fermenter.

A

TCBS agar

58
Q

What is the color of sucrose fermenting spp. Of Vibrio?

A

Yellow

59
Q

Vibrio spp. that are sucrose fermenter.

A

V. cholerae
V. alginolyticus

60
Q

Color of Nonsucrose-fermenting Vibrio spp.

A

Green

61
Q

Nonsucrose-fermenting Vibrio spp.

A

V. mimicus
V. parahaemolyticus
V. vulnificus (most strain)

62
Q

The significant of TCBS is to differentiate ____ from ____.

A

V. cholerae (yellow)
V. parahaemolyticus (green)

63
Q

TCBS agar
Yellow:
Green:nonsucrose fermenter
Blue:

A

Yellow-acidic
Blue-alkaline

64
Q

Vibrio: (s) O/129; (+) string test)
Aeromonas:

A

Aeromonas: (R) O/129;(-) string test

65
Q

Vibrio:Inability to ferment inositol
Plesiomonas:

A

Plesiomonas: able to ferment inositol

66
Q

Vibrio: oxidase()
Enterobacteriacieae: oxidase ()

A

Vibrio: oxidase(+)
Enterobacteriacieae: oxidase (-)

67
Q

Vibrio: fermnetative
Pseudomonas:

A

Pseudomonas:non fermentative

68
Q

All are characteristics of Aeromonas spp. Except:
A. Glucose fermenter
B. Motile
C. Straight roda
D. Ubiquitous
E. Oxidase (-)

A

E

69
Q

Awromonas are motile by means of ___

A

Single polar falgellum

70
Q

What are the 2 groups of Aeromonas?

A

Mesophilic group (37°C)
Psychropgylic group (22-25°C)

71
Q

Aeromonas mesophilic group composed of what?

A

A. hydrophilacomplex
A. veronii complex
A. cariae complex

72
Q

The only Aeromonas that is psychrophilic.

A

A. salmonicida

73
Q

Which among the Aeromonas sp. Is nonmotile?
A. A. hydrophilacomplex
B. A. veronii complex
C. A. salmonicida
D. A. cariae complex

A

C.

74
Q

Two infections of Aeromonas.

A

Intestinal infection
Extraintestinal infection

75
Q

Specimen for intestinal infection of aeromonas.

A

Stool

76
Q

Sp. Of Aeromonas most frequently associated with intestinal infection.

A

A. caviae

77
Q

5 diarrheal presentation of Aeromonas intestinal infection.

A
  1. Acute, secretory diarrhea with vomiting
  2. Acute, dysenteric form similar to shigellosis
  3. Chronic diarrhea lasting more than 10 days
  4. Cholera-like disease
  5. Nebulous syndrome (traveler’s diarrhea)
78
Q

Extraintestinal infection of Aeromonas.

A

Septicemia
Menigitis
Wound infection

79
Q

Most invasive type of Extraintestinal infection of Aeromonas.

A

Septicemia

80
Q

Wound infections of Aeromonas is due to __.

A
  1. Traumatic aquatic exposure
  2. Cellulitis
  3. Uses of leech for medical therapy
81
Q

Characteristics of Aeromonas colonies except :
A. Grayish
B. Large
C. Raised
D. Ferment lactose
E. Opaque with an entire edge and smooth,mucoid surface

A

A.

82
Q

T.or F
Aeromonas has an extremely strong odor.

A

True

83
Q

Pigmentation range of Aeromonas.

A

Translucent & white to buff colored

84
Q

Medium for highest recovery of Aeromonas.

A

Ampicillin SBA
Modified CIN (CIN II)

85
Q

Appearance of Aeromonas in Ampicillin SBA and CIN II.

A

Pink-centered colonies

86
Q

Screening procedure for aeromonas

A

Oxidase (+)
Spot indole test (+)
Growth in NB with 0% NaCl

BACTE LESSON 20 (2 decks)

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