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BACTE LESSON 20 > VIBRIO and AEROMONAS > Flashcards

VIBRIO and AEROMONAS Flashcards

1
Q

T or F
Vibrio, Aeromonas,Plesiomonas, Campylobacter are gram (+) cocci.

A

False
Gram(-) bacilli

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2
Q

T or F
Vibrio, Aeromonas, Plesiomonas, Campylobacter are oxidase (+)

A

True

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3
Q

All Bacteria spp. that are commonly found in aquatic environments except:
A. Vibrio
B. Aeromonas
C. Plesiomonas
D. Campylobacter

A

D

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4
Q

Different significant Vibrio spp.

A

V. cholerae
V. parahaemolyticus
V. vulnificus
V. alginolyticus

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5
Q

Most important and common sp. Of Vibrio.

A

Vibrio cholerae

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6
Q

All are factors of Vibrio infection except:
A. Recent consumption of raw seafood (oyster)
B. Eating aquatic plants
C. Exposure of aquatic environment
D. Accidental trauma during contact eith fresh estuarine/marine water
E. Recent immigration/foreign travel

A

B.

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7
Q

Characteristics of Vibrio infection.

A

Gastroenteritis with cholera-like or
Rice water stool

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8
Q

Characteristics of Vibrio under microscope

A
  • Gram (-)
  • Asporogenous
  • Curved
  • comma shaped bacteria
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9
Q

Characteristic of Vibrio in broth media

A

Possess polar, sheathed flagella

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10
Q

Appearance of Vibrioin solid media

A

Peritrichous, unsheathed flagella

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11
Q

Vibrio spp. are susceptible to what compound? And it is used for confirmation.

A

Vibrio static compound O/129 (150ųg)

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12
Q

Mucoid “stringing” rxn. W/ 0.5% sodium deoxycholate.

A

Positive string test

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13
Q

All Vibrio spp. are halophilic except:

A

V. cholerae
V. mimicus
(They are only found in fresh water)

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14
Q

Major subgroups of V. cholerae.

A

V. cholerae O1
V. cholerae O139
V. cholerae non-O1

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15
Q

Subgroup of V. cholerae that is the most common and cause diarrhea infection.

A

V. cholerae O1

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16
Q

Serotypes of V. cholerae O1

A

Ogawa (A,B)
Inaba (A,C)
Hikojima (A,B,C)

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17
Q

Common cause of Epidemic cholera

A

V. cholerae O1
V. cholerae O139

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18
Q

Causative agent of cholera, specifically Asiatic cholera and Epidemic cholera.

A

Vibrio cholerae

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19
Q

Acute diarrheal disease brought by V. cholerae spread through ___.

A

Contaminated water

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20
Q

Watery with numerous flecks of mucus ;10-30 per day

A

Rice-water stool

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21
Q

What do you call the enterotoxin of V. cholerae?

A

Cholera toxin /choleragen

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22
Q

The enterotoxin of V. cholerae stimulates production of ___.

A

Adenylate cyclase

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23
Q

T or F
V. cholerae enterotoxin can cause hypersecretion of electrolytes & water out of the cell.

A

True

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24
Q

Treatment for V. cholerae.

A

IV/oral fluids

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25
V. cholerae is dangerous because it can leads to __.
Dehydration
26
T or F V. cholerae infection is self-limiting.
True
27
Action of V. cholerae leading to toxin is brought by ___.
2 fragments Fragment A and B
28
This fragment activates the enzyme adenylate cyclase (cAMP)
Fragment A
29
It facilitates the entry of Fragment A.
Fragment B
30
How does the hypersecretion of electrolytes and water happens?
Due to the accumulation of cAMP
31
Two biogroups of epidemic V. cholerae O1 strains.
Classic El Tor
32
Predominant biogroup of Epidemic V. cholerae O1 strain.
El Tor
33
All are characteristics of El Tor biogroup of V. cholerae O1 except: A. VP(+) B. Non hemolytic C. Inhibit by polymyxin B D. Agglutinates chicken RBCs
B. It hemolyzes RBCs
34
All are characteristics of classic biogroup of V. cholerae O1 strain except: A. Agglutinates chicken RBCs B. VP (-) C. Resistant to (polymyxin B) D. Non hemolytic RBCs
A. It doesn't agglutinates
35
The second most common Vibrio spp. That cause gastroenteritis.
V. parahaemolyticus
36
No. 1 cause of summer diarrhea in Japan.
V. parahaemolyticus
37
This Vibrio is thermophiles
V. parahaemolyticus
38
Virulence is associated with the production of heat-stable hemolysin.
Kanagawa phenomenon
39
In Kanagawa phenomenon, it lyses human RBCs in what agar ?
Wagatsuma agar (BAP)
40
High-salt mannitol medium
Wagatsuma agar
41
T or F V. parahaemolyticus is (+) in kanagawa toxin
True
42
Lactose positive Vibrio (pink in MAC)
V. vulnificus
43
The second most serious type of Vibrio is associated with infections.
V. vulnificus
44
Two categories of infections cause by V. vulnificus.
Primary septicemia Wound infection
45
Infection of V. vulnificus brought by consumption of shellfish.
Primary septicemia
46
Infection of V. vulnificus brought by traumatic aquatic wounds.
Wound infection
47
T or F The most common infection of V. vulnificus is gastroenteritis.
False
48
The least pathogenic and most infrequently isolates sp. of Vibrio.
V. alginolyticus
49
V. alginolyticus is strictly halophile, and what % of NaCl?
1%
50
V. alginolyticus is prone to what type of occupation?
Fishermen and sailors
51
What transport medium is not recommended in Vibrio spp.?
Buffered glycerol saline
52
T or F Vibrio spp. Are fastidious bacteria
False-non fastidious
53
Culture medium for Vibrio should contain at least ___.
O.5% salt
54
All are the characteristics of Vibrio in SBA/CHOC media except: A. Medium-large volonies B. Opaque C. Translucent D. Irridescent with greenish hue E. Smooth
C
55
T or F In MAC, all vibrio spp. are non!Lactose-fermenter except V. cholerae.
False- V. vulnificus
56
Most recommended selective medium for Vibrio spp.
Thiosulfate Citrate Bile salt Sucrose Agar (TCBS)
57
This medium differentiates the sucrose fermenter and non sucrose fermenter.
TCBS agar
58
What is the color of sucrose fermenting spp. Of Vibrio?
Yellow
59
Vibrio spp. that are sucrose fermenter.
V. cholerae V. alginolyticus
60
Color of Nonsucrose-fermenting Vibrio spp.
Green
61
Nonsucrose-fermenting Vibrio spp.
V. mimicus V. parahaemolyticus V. vulnificus (most strain)
62
The significant of TCBS is to differentiate ____ from ____.
V. cholerae (yellow) V. parahaemolyticus (green)
63
TCBS agar Yellow: Green:nonsucrose fermenter Blue:
Yellow-acidic Blue-alkaline
64
Vibrio: (s) O/129; (+) string test) Aeromonas:
Aeromonas: (R) O/129;(-) string test
65
Vibrio:Inability to ferment inositol Plesiomonas:
Plesiomonas: able to ferment inositol
66
Vibrio: oxidase(_) Enterobacteriacieae: oxidase (_)
Vibrio: oxidase(+) Enterobacteriacieae: oxidase (-)
67
Vibrio: fermnetative Pseudomonas:
Pseudomonas:non fermentative
68
All are characteristics of Aeromonas spp. Except: A. Glucose fermenter B. Motile C. Straight roda D. Ubiquitous E. Oxidase (-)
E
69
Awromonas are motile by means of ___
Single polar falgellum
70
What are the 2 groups of Aeromonas?
Mesophilic group (37°C) Psychropgylic group (22-25°C)
71
Aeromonas mesophilic group composed of what?
A. hydrophilacomplex A. veronii complex A. cariae complex
72
The only Aeromonas that is psychrophilic.
A. salmonicida
73
Which among the Aeromonas sp. Is nonmotile? A. A. hydrophilacomplex B. A. veronii complex C. A. salmonicida D. A. cariae complex
C.
74
Two infections of Aeromonas.
Intestinal infection Extraintestinal infection
75
Specimen for intestinal infection of aeromonas.
Stool
76
Sp. Of Aeromonas most frequently associated with intestinal infection.
A. caviae
77
5 diarrheal presentation of Aeromonas intestinal infection.
1. Acute, secretory diarrhea with vomiting 2. Acute, dysenteric form similar to shigellosis 3. Chronic diarrhea lasting more than 10 days 4. Cholera-like disease 5. Nebulous syndrome (traveler's diarrhea)
78
Extraintestinal infection of Aeromonas.
Septicemia Menigitis Wound infection
79
Most invasive type of Extraintestinal infection of Aeromonas.
Septicemia
80
Wound infections of Aeromonas is due to __.
1. Traumatic aquatic exposure 2. Cellulitis 3. Uses of leech for medical therapy
81
Characteristics of Aeromonas colonies except : A. Grayish B. Large C. Raised D. Ferment lactose E. Opaque with an entire edge and smooth,mucoid surface
A.
82
T.or F Aeromonas has an extremely strong odor.
True
83
Pigmentation range of Aeromonas.
Translucent & white to buff colored
84
Medium for highest recovery of Aeromonas.
Ampicillin SBA Modified CIN (CIN II)
85
Appearance of Aeromonas in Ampicillin SBA and CIN II.
Pink-centered colonies
86
Screening procedure for aeromonas
Oxidase (+) Spot indole test (+) Growth in NB with 0% NaCl

BACTE LESSON 20 (2 decks)

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