PLESIOMONAS and CAMPYLOBACTER

Question 1

The only member of the Family Enterobacteriacieae that is oxidase (+).

Answer 1

Plesiomonas

Question 2

Characteristics of Plesiomonas

Answer 2

• glucose fermenter
• facultative anaerobes
• motile (polar flagella)
• gram (-) bacilli

Question 3

Only sp. Of Plesiomonas found in Estuarine and fresh water

Answer 3

P. shigelloides

Question 4

Infections of Plesiomonas

Answer 4

Gastroenteritis
Extraintestinal infection

Question 5

3 major clinical types of gastroenteritis associated with Plesiomonas.

Answer 5

-commonly watery/secretory diarrhea
- subacute/chronic disease (14 days-2-3months)
-. More invasive/dysenteric form

Question 6

Extraintestinal infection brought by Plesiomonas.

Answer 6

Bacteremia
Meningitis (occupational exposure)

Question 7

Characteristics of Plesiomonas except
A. Straight
B. No spore/capsule
C. Gram (-) bacilli
D. Nonmotile

Answer 7

D

Question 8

Plesiomonas is motile due to__

Answer 8

Monotrichous or
2-5 lopotrichous flagella

Question 9

T or F
Plesiomonas shares identical biochemical & antigenic features with Klebsiella

Answer 9

False: Shigella

Question 10

Characteristics of Plesiomonas colonies.

Answer 10

Shiny
Opaque
Nonhemolytic
Slightly raised center
Smooth and entire edge

Question 11

T or F
Plesiomonas a delay Lactose fermenter.

Answer 11

True

Question 12

Recommend medium for Plesiomonas.

Answer 12

Inositol brillant green bile salt agar

Question 13

Appearance of Plesiomonas in Inositol brillant green bile salt agar.

Answer 13

White to pink colonies

Question 14

T or F
Plesiomonas will not grow in CIN but will grow in TCBS.

Answer 14

False: Plesiomonas grow in CIN not in TCBS (Only Vibrio)

Question 15

An oxidase (+) and gram (-) bacilli that is not usually found in aquatic environment.

Answer 15

Campylobacter

Question 16

Known to cause abortion in domestic animals

Answer 16

Campylobacter

Question 17

Sp. of Campylobacter, that is the most common cost of bacterial gastroenteritis worldwide.

Answer 17

C. jejuni

Question 18

Transmission of Campylobacter

Answer 18

Direct contact with animals
Consumption of contaminated water and foods

Question 19

Campylobacter plays role in ____ (acute paralysis)

Answer 19

Guillain-Barre Syndrome

Question 20

Major cause of type B gastritis (inflammation of intestine)

Answer 20

Helicobacter pylori

Question 21

Important risk factors of gastric ulcer and gastric carcinoma

Answer 21

H. pylori

Question 22

Campylobacter sp. That is common found in blood culture.

Answer 22

C. fetus subsp. fetus

Question 23

Campylobacter spp. That cause enteric illness (stool)

Answer 23

C. jejuni

Question 24

It is toxic in Campylobacter spp.

Answer 24

Buffered glycerol-saline

Question 25

Tissue samples.for H. pylori may be transported in ____

Answer 25

Cysteine Brucella broth w/ 20% glycerol and frozen at -70°C

Question 26

Different culture media for Campylobacter

Answer 26

CAMPY-BAP Butzler medium Skirrow's medium CCDA (charcoal cefoperazone deoxycholate agar)

Question 27

Incubation temp. For C. jejuni

Answer 27

42°C

Question 28

Incubation temp for C. fetus subsp. fetus.

Answer 28

37°C

Question 29

Similarities of Campylobacter and Helicobacter

Answer 29

Microaerophilic and Capnophilic environment (low. Conci. Of Oxygen and req. increase CO2

Question 30

Gas mixture of Campylobacter

Answer 30

5% O2 10% CO2 85% N2

Question 31

Gas mixture of Helicobacter

Answer 31

5-10% O2 5-12% CO2

Question 32

Presumtive identification -curved, non-spore forming, gram(-) rods

Answer 32

Campylobacter

Question 33

Presumptive identification: Long spiral, S shape, Seagull wings shape

Answer 33

Enteric Campylobacter

Question 34

Recommended counterstain in Campylobacter

Answer 34

Carbolfuchsin

Question 35

Characteristics of Campylobacter except: A. Darting motility B. Moist C. Opaque D. Non hemolytic E. Tan/slightly pink

Answer 35

C.

Question 36

Definitive Identification of Campylobacter.

Answer 36

Oxidase (+) Motility: Brucella/tryptic soy broth Hippurate hydrolysis (+)

Question 37

Definitive Identification of Helicobacter.

Answer 37

- Rapid urease (+) - Christensen's urea medium and incubate at 37°C for 2hrs. - Urea breath test(13C/14C labeled urea orally) 13CO2 and 14CO2 is exhaled