PLESIOMONAS and CAMPYLOBACTER Flashcards
The only member of the Family Enterobacteriacieae that is oxidase (+).
Plesiomonas
Characteristics of Plesiomonas
- glucose fermenter
- facultative anaerobes
- motile (polar flagella)
- gram (-) bacilli
Only sp. Of Plesiomonas found in Estuarine and fresh water
P. shigelloides
Infections of Plesiomonas
Gastroenteritis
Extraintestinal infection
3 major clinical types of gastroenteritis associated with Plesiomonas.
-commonly watery/secretory diarrhea
- subacute/chronic disease (14 days-2-3months)
-. More invasive/dysenteric form
Extraintestinal infection brought by Plesiomonas.
Bacteremia
Meningitis (occupational exposure)
Characteristics of Plesiomonas except
A. Straight
B. No spore/capsule
C. Gram (-) bacilli
D. Nonmotile
D
Plesiomonas is motile due to__
Monotrichous or
2-5 lopotrichous flagella
T or F
Plesiomonas shares identical biochemical & antigenic features with Klebsiella
False: Shigella
Characteristics of Plesiomonas colonies.
Shiny
Opaque
Nonhemolytic
Slightly raised center
Smooth and entire edge
T or F
Plesiomonas a delay Lactose fermenter.
True
Recommend medium for Plesiomonas.
Inositol brillant green bile salt agar
Appearance of Plesiomonas in Inositol brillant green bile salt agar.
White to pink colonies
T or F
Plesiomonas will not grow in CIN but will grow in TCBS.
False: Plesiomonas grow in CIN not in TCBS (Only Vibrio)
An oxidase (+) and gram (-) bacilli that is not usually found in aquatic environment.
Campylobacter
Known to cause abortion in domestic animals
Campylobacter
Sp. of Campylobacter, that is the most common cost of bacterial gastroenteritis worldwide.
C. jejuni
Transmission of Campylobacter
Direct contact with animals
Consumption of contaminated water and foods
Campylobacter plays role in ____ (acute paralysis)
Guillain-Barre Syndrome
Major cause of type B gastritis (inflammation of intestine)
Helicobacter pylori
Important risk factors of gastric ulcer and gastric carcinoma
H. pylori
Campylobacter sp. That is common found in blood culture.
C. fetus subsp. fetus
Campylobacter spp. That cause enteric illness (stool)
C. jejuni
It is toxic in Campylobacter spp.
Buffered glycerol-saline
Tissue samples.for H. pylori may be transported in ____
Cysteine Brucella broth w/ 20% glycerol and frozen at -70°C
Different culture media for Campylobacter
CAMPY-BAP
Butzler medium
Skirrow’s medium
CCDA (charcoal cefoperazone deoxycholate agar)
Incubation temp. For C. jejuni
42°C
Incubation temp for C. fetus subsp. fetus.
37°C
Similarities of Campylobacter and Helicobacter
Microaerophilic and Capnophilic environment (low. Conci. Of Oxygen and req. increase CO2
Gas mixture of Campylobacter
5% O2
10% CO2
85% N2
Gas mixture of Helicobacter
5-10% O2
5-12% CO2
Presumtive identification
-curved, non-spore forming, gram(-) rods
Campylobacter
Presumptive identification:
Long spiral, S shape, Seagull wings shape
Enteric Campylobacter
Recommended counterstain in Campylobacter
Carbolfuchsin
Characteristics of Campylobacter except:
A. Darting motility
B. Moist
C. Opaque
D. Non hemolytic
E. Tan/slightly pink
C.
Definitive Identification of Campylobacter.
Oxidase (+)
Motility: Brucella/tryptic soy broth
Hippurate hydrolysis (+)
Definitive Identification of Helicobacter.
- Rapid urease (+)
- Christensen’s urea medium and incubate at 37°C for 2hrs.
- Urea breath test(13C/14C labeled urea orally)
13CO2 and 14CO2 is exhaled
