PLESIOMONAS and CAMPYLOBACTER Flashcards

1
Q

The only member of the Family Enterobacteriacieae that is oxidase (+).

A

Plesiomonas

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2
Q

Characteristics of Plesiomonas

A
  • glucose fermenter
  • facultative anaerobes
  • motile (polar flagella)
  • gram (-) bacilli
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3
Q

Only sp. Of Plesiomonas found in Estuarine and fresh water

A

P. shigelloides

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4
Q

Infections of Plesiomonas

A

Gastroenteritis
Extraintestinal infection

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5
Q

3 major clinical types of gastroenteritis associated with Plesiomonas.

A

-commonly watery/secretory diarrhea
- subacute/chronic disease (14 days-2-3months)
-. More invasive/dysenteric form

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6
Q

Extraintestinal infection brought by Plesiomonas.

A

Bacteremia
Meningitis (occupational exposure)

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7
Q

Characteristics of Plesiomonas except
A. Straight
B. No spore/capsule
C. Gram (-) bacilli
D. Nonmotile

A

D

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8
Q

Plesiomonas is motile due to__

A

Monotrichous or
2-5 lopotrichous flagella

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9
Q

T or F
Plesiomonas shares identical biochemical & antigenic features with Klebsiella

A

False: Shigella

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10
Q

Characteristics of Plesiomonas colonies.

A

Shiny
Opaque
Nonhemolytic
Slightly raised center
Smooth and entire edge

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11
Q

T or F
Plesiomonas a delay Lactose fermenter.

A

True

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12
Q

Recommend medium for Plesiomonas.

A

Inositol brillant green bile salt agar

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13
Q

Appearance of Plesiomonas in Inositol brillant green bile salt agar.

A

White to pink colonies

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14
Q

T or F
Plesiomonas will not grow in CIN but will grow in TCBS.

A

False: Plesiomonas grow in CIN not in TCBS (Only Vibrio)

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15
Q

An oxidase (+) and gram (-) bacilli that is not usually found in aquatic environment.

A

Campylobacter

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16
Q

Known to cause abortion in domestic animals

A

Campylobacter

17
Q

Sp. of Campylobacter, that is the most common cost of bacterial gastroenteritis worldwide.

A

C. jejuni

18
Q

Transmission of Campylobacter

A

Direct contact with animals
Consumption of contaminated water and foods

19
Q

Campylobacter plays role in ____ (acute paralysis)

A

Guillain-Barre Syndrome

20
Q

Major cause of type B gastritis (inflammation of intestine)

A

Helicobacter pylori

21
Q

Important risk factors of gastric ulcer and gastric carcinoma

A

H. pylori

22
Q

Campylobacter sp. That is common found in blood culture.

A

C. fetus subsp. fetus

23
Q

Campylobacter spp. That cause enteric illness (stool)

A

C. jejuni

24
Q

It is toxic in Campylobacter spp.

A

Buffered glycerol-saline

25
Q

Tissue samples.for H. pylori may be transported in ____

A

Cysteine Brucella broth w/ 20% glycerol and frozen at -70°C

26
Q

Different culture media for Campylobacter

A

CAMPY-BAP
Butzler medium
Skirrow’s medium
CCDA (charcoal cefoperazone deoxycholate agar)

27
Q

Incubation temp. For C. jejuni

A

42°C

28
Q

Incubation temp for C. fetus subsp. fetus.

A

37°C

29
Q

Similarities of Campylobacter and Helicobacter

A

Microaerophilic and Capnophilic environment (low. Conci. Of Oxygen and req. increase CO2

30
Q

Gas mixture of Campylobacter

A

5% O2
10% CO2
85% N2

31
Q

Gas mixture of Helicobacter

A

5-10% O2
5-12% CO2

32
Q

Presumtive identification
-curved, non-spore forming, gram(-) rods

A

Campylobacter

33
Q

Presumptive identification:
Long spiral, S shape, Seagull wings shape

A

Enteric Campylobacter

34
Q

Recommended counterstain in Campylobacter

A

Carbolfuchsin

35
Q

Characteristics of Campylobacter except:
A. Darting motility
B. Moist
C. Opaque
D. Non hemolytic
E. Tan/slightly pink

A

C.

36
Q

Definitive Identification of Campylobacter.

A

Oxidase (+)
Motility: Brucella/tryptic soy broth
Hippurate hydrolysis (+)

37
Q

Definitive Identification of Helicobacter.

A
  • Rapid urease (+)
  • Christensen’s urea medium and incubate at 37°C for 2hrs.
  • Urea breath test(13C/14C labeled urea orally)
    13CO2 and 14CO2 is exhaled