Vibria (cholerae)

Question 1

Morphology Vibrio

Answer 1

gram -
CURVES rods ( comma)
O antigen
H antigen ( flagellum)
non-spore-forming

Question 2

Cultivation

Answer 2

Fac. anaerobic
salt need and tolerance
Acid labile

Question 3

Pathogeny

Answer 3

Obligate and opportunistic pathogens

Question 4

Pathogeny vibrio cholerae

Answer 4

obligate human pathogen

Question 5

Virulence factors vibrio cholerae

Answer 5

a) cell-bound: LPS, toxin-coregulated pili (TCP)
b) enzymes: mucinase, neuraminidase, protease
c) exotoxins: lysogenic conversion

cholera toxin
- AB5 toxin – ADP-ribosyltransferase → enhances activity of adenylate cyclase → K+, Cl- and HCO3- hypersecretion, water loss
=> large-scale watery diarrhoea

zonula occludens toxin (Zot)

accessoricus cholera enterotoxin (Ace)

Question 6

Diseases caused by cholera

Answer 6

watery, rice-water like stool.
Metabolic acidosis(HCO3- loss)
Arrythmia ( K+ loss)

Question 7

Immunity Cholera

Answer 7

IgA based

Question 8

Diagnosis cholera

Answer 8

onto selective TCBS and enrichment (alkaline pepton water) culture media

species: MALDI-TOF
Serological: agglutination based on O antigen

Question 9

therapy cholera

Answer 9

doxycycline

trimethoprim-sulfamethoxazole

Question 10

pathogeny vibria parahaemolyticus

Answer 10

obligate pathogen

Question 11

diseases caused by vibria parahaemolyticus

Answer 11

watery diarrhoea

found in undercooked seafood

Question 12

biochemical activity

Answer 12

B-haemolysis

Question 13

Morphology v. vulnificus

Answer 13

capsulated

Question 14

pathogeny v. vulnificus

Answer 14

obligate pathogen

Question 15

diseases caused by v.vulnificus

Answer 15

soft tissue infections
wound infections
cullulitis
sepsis

Question 16

therapy v. vulnificus

Answer 16

minocycline+ fluoroquinolon

cephalosporins