Vectors - Plasmid and Bacteriophage

Question 1

What are plasmid vectors?

Answer 1

They are small circular, double-stranded circular DNA molecules present in bacteria

Question 2

Characteristics of plasmid vectors:

They are replicated _______, they can carry ______________ genes.

Answer 2

they are replicated autonomously and can carry antibiotic resistance genes

Question 3

The essential assets of a functional plasmid (3)

Answer 3

1. Ori
2. Amp
3. Polylinker

Question 4

What does the origin of replication do?

Answer 4

It mediates replications independently of bacterial chromosomal DNA

Question 5

What is Amp and what does it do?

Answer 5

Amp is a selective marker that allows for the selection of bacteria that carry it

Question 6

What is a polylinker

Answer 6

unique digestive sites - that allow for insert integration, keep plasmid integrity

Question 7

Non - directional cloning

Answer 7

Purpose to store gene X into bacteria

- Direction of insertion does NOT matter (both carry the same DNA sequence)

Question 8

Directional cloning

Answer 8

Direction 1 - (+ strand downstream of promtoer) will express protein of interest

Direction 2 - (- strand dwonstream of promoter) will not express the protein of interest (the insert doesnt match up)