DNA sequencing Flashcards
What is DNA sequencing
DNA sequencing is the process of determine the sequence of nucleotides
What is the purpose of Sanger sequencing?
To identify exact sequence of bases
Techniques involved in the sanger sequencing
DNA copying - fragment production - addition of dideoxy- nucleotides - gel electrophoresis to identify the dideoxy- nucleotides - results showed on chromatogram
True or False - nucleotides can be added to a strand after a dideoxy nucleotide is added
False
What is the difference between a dideoxy nucltoide and regular nucleotide
they lack a hydroxyl group on the 3’ carbon of the sugar ring
What occurs during genetic engineering?
Inserted recombinant DNA into a different organism/genetically modifying organism (GMOP)
What is genetic engineering used for?
to express a protein of interest, get better protein production, resistance to pests and new favourable traits.
What is a transgene
a genetically engineered DNA that is created with a purpose to be introduced to a genome
A functional copy of a gene to rescue a phenotype of a mutant in that gene
Difference between transgender in C.elegans, , Drosophila and mice (mus musclus)
C.elegans - do not integrate but form arrays of repetitive transgender sequence
Drosophila - introduced into a P-element and injected into the embryo
Mice - transgene introduce into a fertilized oocyte by injection or to embyronic stem cells which are incorporated toan embryo
What is requried for agrobacterium infection
the T-DNA - induces proliferation of plant tissue (this is the Ti plasmid vector)
What does the Ti plasmic vector contain?
contains all elements to promote the insertion of the cloned sequence to the host genome and selective markers
What is a transgenic plant
Plants that have one or more genes from another species that have been introduced into the genome
