Vaccines Flashcards
What are some risks with current live vaccines
Vaccine may be pathogenic, disease attributable to contamination
What do you need to do to design molecular vaccines
Identify: protective antigens, protective immune responses, genes encoding protective antigens
Deliver protective antigens
What is the ranking for most difficult to make vaccines for to least
Eukaryotes>bacteria>viruses
What is one way to identify protective immune mechanisms
In vitro assays with antibody
How do you identify gene encoding for protective protein
Antibody screening of DNA library of organism in expression vector
What are the three classifications of recombinant vaccines
1: Subunit
2: gene deleted
3: Live vectored vaccines
What is the most risky classification of recombinant vaccine
Live vectored vaccines
What are three ways sub-unit vaccines are made by cells
inside cell, on cell membrane, made inside of cell and excreted
Are DNA vaccines replicating
They are non-replicating
What does a chimera do
Express different antigens
What is the difference between category 2 and 3 vaccines
Category 2 is only removing part, category 3 also adds
Why do you want to have new molecular vaccines
There are risks with current live, improve quality control, minimize side effects
