V. Amylase Kinetics

Question 1

how to plot michaelis menten curve + equation

Answer 1

X-axis = substrate concentration [S]
Y-axis = absorbance [ABS]

y = ax² + bx + c

Question 2

whats Km

Answer 2

Km (Michaelis constant): the substrate concentration at which the reaction speed is half of Vmax

Question 3

whats Vmax

Answer 3

maximum speed of the reaction (when all enzyme is saturated with substrate)

Question 4

interpretation of Km (low/high)

Answer 4

Low Km → enzyme has high affinity for the substrate → works well even at low substrate (not much substrate left)

High Km → enzyme has low affinity → needs a lot of substrate to work efficiently (lots of substrate left)

Question 5

how to plot lineweaver burk plot + equation

Answer 5

X-axis = 1 / [S]
Y-axis = 1 / ABS

y = mx + c

Question 6

slope formula

Answer 6

Km / Vmax

Question 7

Vmax formula

Answer 7

1 / y-intercept

Question 8

Km formula

Answer 8

• 1 / x-intercept

Question 9

alternative equation of lineweaver burk

Answer 9

1/v = (Km/Vmax)(1/[S]) + 1/Vmax