II. Amylase Characterisation Flashcards
1
Q
[substrate]? + amylase –> tested with [reagent]?
A
amylum, DNS
2
Q
principle of DNS?
A
tests conc/abs of product (reducing sugars)
3
Q
DNS stands for
A
3,5-dinitrosalicylic acid
4
Q
(+) result? (abs, product, reactant)
A
high abs = lots of reducing sugars = lots of amylum broken down
5
Q
(-) result? (abs, product, reactant)
A
low abs = not many reducing sugars = not much amylum broken down
6
Q
optimum pH of a amylase
A
6.7 - 7.0
7
Q
optimum temp of a amylase
A
37C
8
Q
pH too high/low?
A
denatures
9
Q
temp too high/low?
A
high: denatures
low: reaction rate slows
10
Q
types of amylase
A
a amylase
b amylase
glucoamylase
11
Q
product(s) of a amylase
A
glucose, maltose, maltotriose, dextrins
12
Q
product(s) of b amylase
A
maltose
13
Q
product(s) of glucoamylase
A
glucose
14
Q
nm to measure DNS
A
A540
15
Q
nm to measure iodine
A
A590
