UV-Vis Spectrophotometry Flashcards
1
Q
What is spectrophotometry?
A
The measurement of “light” (electromagnetic radiation) either absorbed, emitted, or scattered
2
Q
What is Planck’s Law?
A
E = hv = h(c/lambda)
3
Q
What are complimentary colours?
A
Opposites in the colour wheel
4
Q
Why are objects coloured?
A
They are made up of atoms which all have different electron configurations that absorb and/or re-emit photons in specific wavelengths.
The colour of an object corrresponds to the complimentary colour of the absorbed wavelength
5
Q
What is the Lambert-Beer Law?
A
Aλ = ελ × C × l
The absorbance of a sample at a specific wavelength = Extinction coefficient x Molarity x Length of cuvette
