UV Light/Kirby/Antiseptics & Disinfectants Flashcards
Order of light in Electro Magnetic spectrum
Longer waves = lower frequency
Radio - Microwave - Infrared- Visible - UV - Xray- Gamma Ray
Shorter waves = higher frequency
Long to short wavelengths (in nm)
RMIVUXG (Restless Maids In Venice Use Xbox Games for entertainment)
Non ionizing Radiation (*UV)
Disinfection of surfaces, water, AC systems, surgeries
DNA absorbs energy
Form pyrimidine dimers (thymine dimers)
Abnormal bonds - structure of DNA changes & causes mutations
Ionizing radiation
Form ions - fragment/break DNA
DNA hit by ionizing radiation, bonds broken, forming ions
Sterilization
Can damage DNA & cause mutations
UV light disadvantages
- Over exposure can lead to cancer
* Sun, tanning beds, nail salons
Antiseptic
Applied directly to exposed body surfaces to inhibit vegetative pathogen growth - isopropyl + hydrogen peroxide
Disinfectant
Applied to surface
Iodine/alcohol can be both antiseptic or disinfectant
Targetting
Cell wall - kill Gram +
Membranes -susceptibility to death of Gram -
Proteins-both Gram +/-
Zone of inhibition
Measured diameter; clear area/no growth due to chemical
Large zone size
Small zone size
More susceptible bacteria is to chemical agent
More resistant to chemical agent
Don’t forget our Gram - & +
Gram + = S. epidermidis/ S.aureus
Gram -= S. marcescens/ P.aeruginosa/E.coli
Antibiotic
Destroy microorganisms/antimicrobial
Kirby Bauer
Antimocrobial susceptibility
Culture on agar, antimocrobial disc placed, diffusion of antimicrobial chemical into surrounding agar, clear area
S = susceptibility
R= resistant
I=internediate
Staphyloccocus /Bacillus
Similar resistance/susceptibility (gram +)
Don’t forget Bacillus has endospores
Penicillin
Gram + susceptible/cell wall inhibiting (narrow spectrum)
Gebtamycin
Broad spectrum (both gram +/-)
Protein synthesis inhibitor
