Using A Graticule To Calibrate A Light Microscope

Question 1

What’s an eyepiece graticule

Answer 1

Glass disc marked with a fine scale of 1-100. No units and remains unchanged whichever objective lens is in place. The relative size of the divisions, however, increases with each increase in magnification. Need to know what the divisions represent at different magnifications so you can measure specimens.

Question 2

What’s a stage micrometer

Answer 2

A microscope slide with a very accurate scale in micrometers engraved on it. Usually 100 divisions=1mm so 1 division=10 micrometers.