Use Of Spectrophotometry In Enzyme Kinetics

Question 1

In order that a chemical reaction such as A →p should occur

Answer 1

The A molecules must overcome an energy barrier to react
This means that at any given instanta certain fraction of the population of A moleculesmust possess much more energy than the remainder of the population ie enough energy to attain an activated state in which the energy barrier is overcome, chemical bonds are made or broken and the products p formed

Question 2

The term activation energy refers sto

Answer 2

The amount of energy, in calories, required to bring all the molecules of 1 mole of substance at a given temperature to this activated state

Question 3

The rate of reaction will depend on the number of molecules A that

Answer 3

Attain enough energy to pass over the energy barrier

Question 4

In ordinary chemical reactions this activation energy may be supplied by

Answer 4

Heating the reactants

Question 5

Catalysts such as ______ accelerate chemical reactions in dramatic fashion by lowering the ______

Answer 5

Enzymes, activation energy required

Question 6

Enzyme-catalysed reactions are of extreme important because

Answer 6

Advantage of specificity / important to the economy of the cell - metabolic regulation whereby the cell can speed up or slow Dow n to nil the activities of enzymes as the demands of the cell dictate

Question 7

Henry (1902) / Michael and mentor (1913) and brings and haldane (1925) pictured a simple enzyme (e) as forming with its substrate (5) a complex Es which could

Answer 7

Dissociate to reform free enzyme (e) and substrates or it could break down to yield the product (p) and regenerate enzyme

Question 8

Spectrophotometer is used as a means of measuring some of the factors that affect the velocity of enzyme catalysed reactions what factors?

Answer 8

Concentration of the enzyme catalysing the reaction, the reaction time and pl of the reaction medium