Determination Of The Activity Of Alkaline Phosphatase

Question 1

Alkaline phosphates is an enzyme which occurs

Answer 1

In the bone, milk, kidney, intestine and certain mo’s

Question 2

Alkaline phosphates has an optimum ph of

Answer 2

Around 10

Question 3

Inhibited by

Answer 3

Chelating agents and by inorganic phosphate, while it requires mg2+ for full activity

Question 4

The bovine intestinal enzyme has a molecular weight of

Answer 4

100,000

Question 5

Human alkaline phosphates in serum is elevated during

Answer 5

During skeletal growth in childhood and adolescence, in Paget’s disease, hyperparathyroidism, rickets and bone tumours

Question 6

The presence of the enzyme in milk is used for

Answer 6

Quality control purposes since the enzyme should be destroyed by ordinary pasteurisation temperatures, the enzyme though may regenerate or reactivate under suitable conditions

Question 7

The enzyme has a role in bone formation, where it probably causes a

Answer 7

Localised high concentration of inorganic phosphate by hydrolysing organic phosphates

Question 8

It hydrolyses a widerange of natural and artificial phosphate esters including p-nitro yielding

Answer 8

An alcohol and orthophosphate

Question 9

P-nitrophenylphosphate, the alcohol, p-nitrophenal absorb strongly in alkali at

Answer 9

405 Nm and colormetrically at 430 Nm as the nitrophenolate ion

Question 10

P-nitrophenylphosphate + H2O → p-nitrophenol + H3P04

Question 11

The enzyme exhibits

Answer 11

Polymorphism

Question 12

Polymorphism can be separated by

Answer 12

Electrophoretically orby ion exchange chromatography on De - Ae cellulose

Question 13

Analysis of the serum isoenzyme pattern can’t used to distinguish

Answer 13

Liver lesions from bone lesions sin the case of metastatic carcinoma

Question 14

The enzyme has a reactive serine residue at the

Answer 14

Active site

Question 15

The serine residue is phosphorylated by the substrate yielding a phosphorylated derivative which then

Answer 15

Decomposes to orthophosphate plus a free serine residue

Question 16

The last reaction is reversible which explains the inhibitory effect of

Answer 16

Orthophosphate

Question 17

The enzyme also has transferase activity

Answer 17

Ie can catalyse the transfer of phosphors framone molecule to another

Question 18

The 0.02 M
Naoh is added to

Answer 18

Stop the enzymic reaction - doesn’t affect intensity of yellow colour

Question 19

The he product of hydrolysis of p-mitrophenylphosphatase is a ph
Indicator with a pk of

Answer 19

7.1 at ph 10 will be over 99.9% in yellow p-nitro form

Question 20

Find u moles mm to m / 1000 → ml to L / 1000
Moles = mxl
Moles →u=x 1 x 10^6