Use Of Microscope Flashcards

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1
Q
  1. What is the primary purpose of a microscope?A) To observe large objectsB) To magnify images of objectsC) To reflect lightD) To capture images digitally
A

Answer: BExplanation: A microscope magnifies images of objects enabling detailed observation and analysis at a scale that would be impossible with the naked eye.

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2
Q
  1. Which of the following microscopes uses transmitted or reflected light?A) Electron microscopeB) Acoustic microscopeC) Optical microscopeD) Scanning tunneling microscope
A

Answer: CExplanation: Optical microscopes use light (transmitted or reflected) to produce images unlike electron microscopes that use electron beams.

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3
Q
  1. What type of lens does a simple microscope use?A) Objective lensB) Eyepiece lensC) Single or compound lensD) Condenser lens
A

Answer: CExplanation: A simple microscope like a magnifier or loupe typically uses a single or compound lens to magnify objects.

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4
Q
  1. In a compound microscope the objective lens is positioned:A) Close to the eyepieceB) Close to the object being examinedC) Below the condenserD) Above the light source
A

Answer: BExplanation: The objective lens in a compound microscope is placed close to the object to create a real image which is then magnified by the eyepiece.

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5
Q
  1. Which component in a compound microscope controls the amount of light entering the condenser?A) Objective lensB) Iris diaphragmC) Mechanical stageD) Fine adjustment knob
A

Answer: BExplanation: The iris diaphragm regulates light entering the condenser essential for controlling illumination on the specimen.

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6
Q
  1. What is the role of the condenser in a microscope?A) To magnify the specimenB) To support the microscope’s armC) To collect and direct light onto the slideD) To provide structural support
A

Answer: CExplanation: The condenser gathers light from the source and focuses it on the specimen ensuring even illumination.

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7
Q
  1. Which microscope allows each eye to view the object separately creating a 3D effect?A) Compound microscopeB) Stereoscopic microscopeC) Polarizing microscopeD) Metallographic microscope
A

Answer: BExplanation: Stereoscopic microscopes allow each eye to view the object separately giving a perception of depth and a 3D appearance.

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8
Q
  1. The type of microscope that uses an annular stop in its diaphragm is the:A) Compound microscopeB) Phase-contrast microscopeC) Polarizing microscopeD) Metallographic microscope
A

Answer: BExplanation: The phase-contrast microscope uses an annular stop in its diaphragm to enhance contrast for transparent unstained specimens.

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9
Q
  1. What feature is specific to polarizing microscopes?A) They magnify using sound wavesB) They have a phase plate for light diffusionC) They measure details of polarization effectsD) They are used only with live specimens
A

Answer: CExplanation: Polarizing microscopes are equipped to measure polarization effects often used to study crystals and detailed structures like bone.

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10
Q
  1. In microscopy what does ‘X’ symbolize?A) Resolving powerB) Magnifying powerC) Numerical apertureD) Virtual image
A

Answer: BExplanation: The symbol ‘X’ represents the magnification power of a lens indicating how many times larger the lens can make an object appear.

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11
Q
  1. What is the primary function of the objective lens in a compound microscope?A) To magnify the virtual imageB) To create a real image in front of the eyepieceC) To collect and direct light from the specimenD) To control the amount of light entering the microscope
A

Answer: BExplanation: The objective lens in a compound microscope forms a real image in front of the eyepiece which is then magnified by the eyepiece for viewing.

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12
Q
  1. Which of the following best describes the purpose of the condenser in a microscope?A) Controls magnification powerB) Focuses light onto the specimenC) Holds the slide in placeD) Houses the eyepiece
A

Answer: BExplanation: The condenser directs and focuses light from the microscope’s light source onto the specimen to ensure a clear image is formed.

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13
Q
  1. The phase-contrast microscope is primarily used to observe which type of structures?A) Thick opaque objectsB) Stained transparent structuresC) Unstained transparent structuresD) Very small objects like atoms
A

Answer: CExplanation: Phase-contrast microscopes are ideal for viewing unstained transparent structures such as living cells by enhancing contrast without needing stains.

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14
Q
  1. In a stereoscopic microscope why does the specimen appear three-dimensional?A) The lens magnifies the object from two anglesB) It uses two objective lenses and two eyepieces for separate imagesC) It has a higher magnification powerD) It uses a phase plate
A

Answer: BExplanation: A stereoscopic microscope provides a three-dimensional view by allowing each eye to see the specimen from a slightly different angle using two objective lenses and eyepieces.

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15
Q
  1. What characteristic enables polarizing microscopes to examine crystalline structures?A) Phase plate and annular stopB) Vertical illuminationC) Polarization effectsD) Adjustable iris diaphragm
A

Answer: CExplanation: Polarizing microscopes have features that permit detailed examination of crystals through their ability to analyze polarization effects.

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16
Q
  1. Which component of a microscope is responsible for holding and adjusting the slide on the stage?A) Mechanical stageB) CondenserC) Fine adjustment knobD) Coarse adjustment knob
A

Answer: AExplanation: The mechanical stage holds the slide in place and allows for precise movements across the stage during examination.

17
Q
  1. The primary advantage of a scanning electron microscope is its ability toA) Produce 3D images of biological specimensB) Image atoms at a molecular levelC) Magnify colored objectsD) Visualize unstained transparent structures
A

Answer: AExplanation: Scanning electron microscopes excel in creating three-dimensional images of surfaces making them ideal for detailed examination of external structures.

18
Q
  1. In a compound microscope the fine adjustment knob is used toA) Sharpen the focus of the specimenB) Move the slide horizontallyC) Adjust the light intensityD) Hold the slide in place
A

Answer: Explanation: The fine adjustment knob refines the focus allowing for sharper and clearer views particularly at higher magnifications.

19
Q
  1. What is the role of immersion oil in microscopy?A) Reduces glareB) Increases resolution by matching refractive indicesC) Enhances magnification powerD) Decreases light absorption
A

Answer: BExplanation: Immersion oil has a refractive index similar to glass which enhances resolution by minimizing the loss of light through refraction allowing finer details to be seen.

20
Q
  1. Which feature of the scanning tunneling microscope allows it to image individual atoms?A) Annular stop and phase plateB) Beam of electrons and condenserC) Electron current and extremely fine tipD) Polarized light and crystals
A

Answer: CExplanation: The scanning tunneling microscope detects electron currents with an ultra-fine tip to image at atomic resolution earning it the

21
Q
  1. What is the maximum magnification power of a compound light microscope typically used for biological specimensA) 50X B) 100X C) 1000X D) 2000X
A

Answer: CExplanation: Compound microscopes generally have a maximum magnification of around 1000X which is sufficient for observing many cellular structures in detail.

22
Q
  1. Which component helps control the amount of light that reaches the specimen in an optical microscope?A) Coarse adjustment knobB) Objective lensC) Iris diaphragmD) Mechanical stage
A

Answer: CExplanation: The iris diaphragm is adjustable allowing the user to control light intensity on the specimen to improve image clarity and contrast.

23
Q
  1. The resolving power of a microscope lens is its ability to A) Create a virtual image B) Differentiate closely spaced points C) Adjust magnification D) Enhance color contrast
A

Answer: BExplanation: Resolving power determines how well a lens can distinguish two points that are close together which is crucial for observing fine specimen details.

24
Q
  1. Which type of microscope is best suited for examining opaque objects like metals?A) Phase-contrast microscopeB) Compound microscope C) Metallographic microscope D) Stereoscopic microscope
A

Answer: C Explanation: Metallographic microscopes utilize vertical illumination making them ideal for examining opaque materials where light must be reflected off the sample surface.

25
Q
  1. The scanning optical microscope is unique becauseA) It operates in reverse compared to a traditional microscopeB) It uses an electron beam to build imagesC) It produces three-dimensional imagesD) It can view atomic structures
A

Answer: AExplanation: In a scanning optical microscope the light source is directed through a condenser onto a small spot allowing a rapid scan of the sample working in reverse compared to traditional microscopes.

26
Q
  1. Describe the process for calculating the magnification power of a lens.
A

Answer: The magnifying power of a lens can be calculated by dividing the least distance of distinct vision (typically 25 cm) by the focal length of the lens. For example a lens with a focal length of 5 cm would have a magnifying power of 5X.

27
Q
  1. Explain the concept of a virtual image as used in microscopy.
A

Answer: In microscopy a virtual image is one that cannot be projected onto a screen but is viewed directly through the eyepiece. It appears as if it is at a different location than it actually is due to the lens bending light rays.

28
Q
  1. What is the importance of immersion oil in microscopy?
A

Answer: Immersion oil is used with high-power objective lenses typically 100X to improve resolution. The oil has a refractive index similar to glass reducing light refraction and allowing more light to pass into the lens resulting in a clearer image.

29
Q
  1. Differentiate between the resolving power and magnification of a microscope.
A

Answer: Magnification refers to how much larger an image appears while resolving power is the ability to distinguish two closely spaced points as separate. Higher resolving power indicates better detail visibility which does not necessarily increase with magnification.

30
Q
  1. Define refractive index in the context of microscopy and explain its significance.
A

Answer: The refractive index is a measure of how much light bends when it enters a material from another medium. In microscopy a higher refractive index helps in better focusing of light into the objective lens enhancing image clarity and resolution.