Use a Light microscope practical

Question 1

first step of practical

Answer 1

Carefully clip slide into stage

Question 2

2nd step of practical

Answer 2

select lowest powered objective lens over the slide

Question 3

3rd step of practical

Answer 3

Use the course adjustment to bring the stage just beneath lens

Question 4

4th step of practical

Answer 4

Look down eye-piece lens(so you don’t damage slide) and gradually move stage downwards using the course adjustment knob. Stop when roughly in focus

Question 5

5th step of the practical

Answer 5

To bring image into proper focus , adjust the fine adjustment knob until a clear image is obtained

Question 6

6th step

Answer 6

To observe the image in higher magnification change to a higher objective lens readjusting the stage using course and fine adjustment knob

Question 7

Why use a thin cell

Answer 7

So light can get through the cell so you can see the cell

Question 8

Whys is the electron microscope better than light

Answer 8

An electron microscope has much higher magnification and
resolving power than a light microscope. This means that it can be
used to study cells in much finer detail. This has enabled biologists
to see and understand many more sub-cellular structures.

Question 9

magnification

Answer 9

The amount that an image of something is scaled up when viewed through a microscope.

Question 10

resolution

Answer 10

the ability of a microscope to distinguish details of a specimen or sample. In other words, the minimum distance between 2 distinct points of a specimen where they can still be seen by the observer or microscope camera as separate entities.

Question 11

Safety:

Answer 11

Take care when handling slides and microscope

Wear gloves if using stains

Wear eye protection

Methylene blue is an irritant