Urine Specimen Flashcards
Urine Containers should be:
- clean, dry, leak-proof containers
- Disposable single-use containers must be used
- Wide mouth and with a wide flat bottom
- Clear material
Recommended capacity specifically for routine urinalysis testing:
50 mL
All specimens must be labeled immediately after collection with:
- Patient’s name, ID number, date, and time of collection should be noted
- Also, patient’s age and location (address) and healthcare provider’s name
- Labels must be attached to the container (not to the lid) and should not detach if the container is refrigerated or frozen
Requisitions may be:
manual (handwritten) or computerized (input, print, and accompanied to the urine specimen)
Requisitions must:
Must accompany specimens delivered to the laboratory
The laboratory should reject specimens that are:
- are unlabeled or improperly labeled
- Labels and requisition forms that do not match
- Specimens contaminated with feces or toilet paper
- Containers with contaminated exteriors
- Specimens of insufficient quantity
- Specimens that have been transported improperly
- Specimens that have not been preserved correctly during a time delay
- Specimens for urine culture collected in a nonsterile container
- Inappropriate collection for the type of testing needed (for example, midstream clean-catch specimen for bacterial culture)
After collection, specimens should be delivered to the laboratory promptly and tested within?
2 hours
A specimen that cannot be delivered and tested within 2 hours should be:
refrigerated or have an appropriate chemical preservative added.
Bacterial growth and precipitation of amorphous material will have a change in what analyte?
Clarity
An analyte _____ will have a multiplication of bacteria that increases the ammonia smell because there is a breakdown of urea into ammonia
Odor
PaBAON: increase or decrease?
Increase
An analyte _____ will have a multiplication of bacteria that has an increased change because there is a breakdown of urea into ammonia due to urease-producing bacteria/loss of CO2
pH
Oxidation to urobilin is a result of a decrease in what analyte?
Urobilinogen
oxidation to biliverdin is a result of a decreased in what analyte?
Bilirubin
what does analytes PaBAON stand for?
pH
Bacteria
Odor
Nitrite
The method of preservation used most routinely is: (why)
refrigeration at 2°C to 8°C,
because: it decreases bacterial growth and metabolism.
If the urine is to be cultured, what should you do?
it should be refrigerated during transit and kept refrigerated until cultured, up to 24 hours
If the urine is subjected to chemical examination using reagent strips, what should you do?
The specimen must return to room temperature before chemical
testing by reagent strips.
why? BECAUSE: the enzyme reactions on the strips perform best at room temperature.
Refrigeration of urine samples can cause precipitation of:
amorphous urate and phosphate crystals
When a specimen must be transported over a long distance and refrigeration is impossible, what should you do?
chemical preservatives may be added.
The ideal preservative should be:
- bactericidal
- inhibit urease
- preserve formed elements in the sediment
- Preservatives should not interfere with chemical tests
REMEMBER: the ideal preservative does not
exist!!!!
therefore, a preservative that best suits the needs of the required analysis should be chosen.
This preservative prevents bacterial growth and metabolism but interferes with the analysis of drugs and hormones
ACIDS!!!!!!
(boric acid, HCL, acetic acid, tartaric acid)
This preservative does not interfere with
chemical tests but precipitates amorphous
phosphates and urates
Refrigeration<3
This preservative is an excellent sediment
preservative but acts as a reducing agent, interfering with chemical tests for glucose, blood, leukocyte esterase, and
copper reduction
Formalin (formaldehyde)
This preservative is good for drug
analyses but Inhibits reagent strip tests for glucose, blood, and leukocytes
Sodium fluoride
BD means?
Becton, Dickinson, from: Rutherford, NJ
used for: Urine Collection Kits
which contain: collection cup, transfer straw, culture and sensitivity (C&S) preservative
tube, or UA tube
Specimen stable at room temperature (RT) for 48 hours:
Light gray and gray C&S tube
Specimen stable at room temperature (RT) for 72 hours:
Cherry red/yellow Preservative Plus tube
preservative used on automated instruments
Yellow UA Plus tube
what preservatives are in the Cherry red/yellow Preservative Plus tube
Preservatives are:
- sodium propionate
- ethyl paraben
- chlorhexidine
what preservatives are in the Light gray and gray C&S tube
Preservatives are:
- boric acid,
- sodium borate
- sodium formate
What type of specimen is/are needed for ROUTINE SCREENING?
- Random
- First morning
- Midstream clean-catch
What type of specimen is/are needed for BACTERIAL CULTURE?
- Catheterized
- Midstream clean-catch
- Suprapubic aspiration