Urinalysis exam 2 Flashcards
How does the pH of urine relate to its hydrogen ion concentration?
There is an inversely proportional relationship between the number of hydrogen ions in solution and the numerical value for pH
Ketone bodies in the urine are suggestive of elevated ________ metabolism.
fat
What causes myoglobinuria?
muscle disorder, muscle trauma, severe crush injury
*extensive muscle injury
What is the general purpose of test strip screening?
Provide info as to whether or not pathological concentrations of substances are present in the urine, and if further clinical testing is necessary in order to confirm a diagnosis suggested by the findings.
What color is a (+) positive nitrite on the dipstick?
pink
What substance is most likely to be present in urine that could have a false (=) negative reaction on the dipstick for glucose?
ascorbic acid(vitamin C)
What is the SSA used for?
*confirm protein in urine
what test is used for detecting reducing sugars in the urine? What would be expected in the microscopic examination?
- clinitest
* nothing
What two dipstick reactions are usually (+) positive in pyelonephritits and cystitis?
- nitrites
* leukocytes
Name several conditions that could be indicated by an elevated urinary urobilinogen.
liver disease (ie: hepatitis, cirrhosis) or hemolytic states (ie: pernicious anemia)
Name several conditions that can cause hemoglobinuria.
intravascular hemolysis conditions (ie: transfusion rxn), extensive burns, strenuous exercise, injury, hemolytic anemia, PNH-paroxysmal nocuturnal hemoglobinuria
What is specificity (as related to dipstick reactions)?
Ability of each test zone to react to a certain substance and being tested and to no other.
The test pad for ketone bodies detects_________?
acetoacetic acid
What is the principal of the dipstick method for protein?
uses protein error of indicators principle - tetrabromphenol blue-buffered @ constant pH 3.0.
Without proteins: yellow
In the presence of proteins: H+ are released by the indicator dye & cause color change (yellow-green to blue-green)
What unusual urinary protein is associated with multiple myeloma?
Bence-Jones
What sugar (especially in infants) is most likely indicated by a (=) negative dipstick reaction for glucose but a (+) clinitest?
galactose
What specimen is used to detect orthostatic proteinuria? When would you suspect this condition?
First morning specimen followed 2 hrs later after being erect/upright for that time; suspect in young children &
adolescence.
What is the active ingredient in Ehrlichs’s reagent?
paradimethylaminobenzaldehyde
What is the reagent used to detect bilirubin in the icotest?
2,4-dichlorobenzene diazonium tetrachlorozincate
The reagent strip for hemoglobin detects _____,______ and _________.
free hemoglobin, myoglobin, intact RBC’s
What is the action of the enzyme peroxidase?
*sudoperoxidase activity catalyzes oxidation reaction of 3,3’,5,5’-tetramethylbenzidine by oxygen released from peroxide
Why is there normally no glucose in the urine?
All filtered glucose is reabsorbed in distal convoluted tubules
What is the advantage of using phase contrast micorscopy in the examination of urinary sediment? How does this elate to the refractive index?
- unstained components and casts
* refractive index is similar to urine
What is the use of the polarizing microscope in urine microscopics?
Detection of birefringent substances (lipids & crystals)
Cells are recorded as number per _____ power field.
High (40X)
How many fields should be examined in a microscope exam?
10
What is the most common type of WBC found in urinary sediment?
neutrophils
Casts in urine are usually accompanied by a (+) test for ______?
protein
Casts containing cells indicate problems in what part of the urinary tract?
kidneys