Urinalysis 3/4 Flashcards
1
Q
How is the sediment prepared for analysis?
A
Mix well, transfer 5ml to centrifuge tube
Centrifuge at 1500rpm for 5 minutes
Decant supernatant with pipette, add stain if desired
Resuspend by flicking until mixed
2
Q
How is the sediment analysed under a microscope?
A
Low power (x10) scanning overview and allow initial quantification of elements (casts, crystals, cells)
High power (x40) allow identification of morphology and bacteria and quantify RBCs and WBCs
3
Q
What cells might you expect to see in a sediment?
A
Erythrocytes
Leukocytes
Squamous/transitional epithelial cells
4
Q
What crystals might you expect to see in a sediment?
A
Magnesium ammonium phosphate (Struvite) Calcium oxalate dihydrate/monohydrate Ammonium biruate Amorphous urates Bilirubin Calcium carbonate
5
Q
What casts might you expect to see in a sediment?
A
Epithelial cell
Leukocyte
Erythrocyte
6
Q
What other things might you expect to see in a sediment?
A
Lipids Yeast/fungi Parasites Spermatozoa Bacteria Contaminants (especially free-catch samples)
