URIC ACID STANBIO Flashcards
Uricase acts upon __________to form ______and
________.
uric acid
hydrogen peroxide
Allantoin
- Uric Acid + H2O + —–> H2O2+ CO2 + Allantoin
what enzyme is used?
Uricase
The ________ is measured quantitatively by its reaction with ________, in the
presence of ________and __________, to form a red violet quinoneimine complex.
H2O2 (hydrogen peroxide)
3,5-dichloro-2-hydroxybenzenesulfonic acid (DCHB)
peroxidase
4-aminophenazone
It is a mixture of special additives developed by Stanbio is integrated into the uric acid reagent to help minimize interference due to lipemia.
Lipid Clearing Factor (LCF)
Red violet complex
N-(4-antipyryl)-3-chloro-5-sulfonate-p-benzoquinonemonoimine+ H2O
2 enzymes used in Uric acid
Uricase and Peroxidase
Reagent stability and storage
2-8°C
Specimen used:
Serum or Plasma (from heparin or EDTA)
Diluted Urine (1:10) 1 part urine, 9 parts of distilled water
Sample Stability
2-3 days = RT
3-7 days = 2-8°C
6-12 months = Frozen
Interfering substances
Hemoglobin = > 100 mg/dl
Bilirubin = >20 mg/dl
How many ul of reagent is pipetted to the cuvets?
1000uL
How many ul of Standard is pipetted in the cuvets
20uL or 0.02 ml
How many ul of sample is pipetted in the cuvets
20uL or 0.02 ml
after pipetting, incubate all cuvettes at?
37°C for 5 minutes and allow to cool, or incubate at room temperature for 15 minutes
Read at what nanometer wavelength
520 nanometers within 15 minutes
