Total Protein

Question 1

Protein molecules contain a large number of ______.

Answer 1

peptide bonds

Question 2

When treated with copper ions (Cu+2) in alkaline solution, a colored complex is formed between the _____ and the _____groups of these peptides.

Answer 2

copper and carbonyl and imine groups

Question 2

When treated with _______ in ________, a
colored complex is formed between the copper and the carbonyl and imine groups of these peptides.

Answer 2

copper ions (Cu+2) in alkaline solution

Question 3

simplest of such compounds formed by heating urea

Answer 3

biuret

Question 5

heating urea, the term ______ was adopted.

Answer 5

“biuret reaction”

Question 6

Method is based on the reports of ______ and _______

Answer 6

Weischselbaum and Gornal et al.

Question 7

The violet color developed is proportional to the
number of peptide bonds in the protein and is nearly independent of the relative concentration of albumin and globulin

Answer 7

TRUE

Question 8

Reagent used in TP

Answer 8

Solution of copper sulfate pentahydrate

other ans:
aqueous sodium hydroxide
potassium iodide and potassium sodium tartrate

Question 9

Total Protein Standard

Answer 9

Aqueous solution of Bovine Albumin, Fraction V, with sodium azide as preservative

Question 10

preservative

Answer 10

sodium azide

Question 11

sodium azide as a preservative. May react with
copper or lead plumbing to form _____

Answer 11

explosive metal azide build up

Question 12

Rgt storage and stability:

Answer 12

Kit ( 2 - 8°C)
Reagent( 2 - 30°C)
Standard ( 2 - 8°C)

Question 13

Specimen Collection/ specimen used

Answer 13

Serum is preferred. Do not use plasma because it has fibrinogen that can increase the results

Question 14

Interfering substances

Answer 14

Gross hemolysis and lipemia.
Not used for urine or spinal fluid

Question 15

Total protein in serum is reportedly stable for
1 week at room temperature (RT), and for 30 days at _______.

Answer 15

2-8°C.

Question 16

How many ul of reagent, sample and standard is pipetted

Answer 16

1000uL or 1.0 ml

Question 17

How many ul of sample and standard is pipetted

Answer 17

10uL or 0.01mL

Question 18

after pipetting, Allow all tubes to stand for at least ________

Answer 18

5 minutes

Question 19

Read S and U vs RB at ___ nm within 1 hour.

Answer 19

550

Question 20

Formula:

Serum Total Protein (g/dL) = Au/ As x 10

Question 21

concentration of STANDARD (g/dL).

Answer 21

10

Question 22

normal range:

Answer 22

6.6 - 8.3 g/dl