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Yasmin Biology flashcards > Unit 6 - practical investigations > Flashcards

Unit 6 - practical investigations Flashcards

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1
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

what needs to be added as a set amount of CO2

A

sodium hydrogen carbonate

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2
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:
what device is used to measure photosynthesis?

A

photosynthometer

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3
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

what plant is used for this investigation?

A

pondweed

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4
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

what are the variables
IV
DV
CV

A

IV= temp/light intensity/ light wavelength, CO2 con
DV= rate of photosynthesis in bubbles produced
CV= amount of time, other possible IVs

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5
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

when testing light intensity change what?

A

distance of lamp from beaker

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5
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:
what to change when measuring wavelength

A

colour filters

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6
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

what do you need to do as soon as bubbles begin to form?

A

start stopwatch + record gas

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7
Q

investigating effect of CO2, light intensity, light wavelength + temp on photosynthesis:

safety?

A

Wear eye protection
Avoid skin contact w sodium hydrogen carbonate
Wash hands w soap
Gloves
Careful; w electric appliances

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8
Q

Core practical 11: use quadrats and transects to determine distribution + abundance of organisms, and measuring abiotic factors appropriate to habitat

when are quadrats used?

A

Choose site in environment w obvious gradient in abiotic factor

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9
Q

Core practical 11: use quadrats and transects to determine distribution + abundance of organisms, and measuring abiotic factors appropriate to habitat

safety?

A

Safety
Beware of allergies to plant
Gloves for skin conditions
Beware of ticks and leeches
Use sunscreen
Wash hands after

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10
Q

Core practical 12: investigate effects of temperature on development of organisms taking into account the ethical use of organisms

what organisms can be used to investigate?

A

brine shrimp
or
seedlings

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11
Q

Core practical 12: investigate effects of temperature on development of organisms (such as seedling growth rate of brine shrimp hatch rates) taking into account the ethical use of organisms

brine shrimp procedure

A

Create salt water solution
Dampen graph paper with salt solution
Pinch small n of brine shrimp eggs + sprinkle onto the damp graph paper
Count 40 eggs (using magnifying glass) and use scissors to cut around eggs on graph paper

Place graph paper discs face down in beakers in dif water bath temperatures

Place each beaker in correct water bath/ refrigerator

Store for 24 hrs
Fill another beaker w salt solution, this beaker will be used to count the brine shrimp

To count the shrimp place a light source near one side of the beaker and when shrimp swim towards light use pipette to take them away
Record n of hatched brine shrimp at each temperature
Repeat counting everyday for 2 days

IV= temperature
DV= n of shrimp
CV= salt water con, time

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12
Q

Core practical 12: investigate effects of temperature on development of organisms (such as seedling growth rate of brine shrimp hatch rates) taking into account the ethical use of organisms

procedure seedlings

A

Procedure: seedlings
Take 5 petri dishes and label them. Place cotton wool at the bottom of each one
Use forceps to count 20 seedlings and make sure they are evenly spread in the petri dish
Add water to each petri dish
Place each petri dish in correct incubator/refrigerator
Leave for 24 hrs , add more water to each petri dish
After 5 days observe the seedlings, and count n that has germinated at each temperature

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13
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

what techniques need to be used when working w microorganisms?

A

aseptic techniques (clean work bench, disinfect equipment using disinfectant, Bunsen burner to flame + convection current)

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14
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

what microorganism is used

A

yeast

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15
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

what medium is used to grow microorganism

A

glucose solution as culture vessel

16
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

how often take samples?

A

at intervals of 20-30 mins, repeat at least 5 times over 12 hrs

17
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

Extension: relating absorbance of light to density of yeast cells in culture:
what can be used to estimate initial density of yeast?

A

Initial density of yeast cells can be estimated using a haemocytometer

18
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

Extension: relating absorbance of light to density of yeast cells in culture:
what is used to stain yeast?

A

methylene blue

19
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

Extension: relating absorbance of light to density of yeast cells in culture:

what to do if cells overlap?

A

dilute the solution

20
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

Extension: relating absorbance of light to density of yeast cells in culture:
calculate vol under 1 field of view

A

Volume under 1 field of view: (area of x40 view / area of coverslip) x volume of 1 drop

21
Q

Core practical 13: investigate the rate of growth of microorganisms in liquid culture, taking into account safe and ethical use of microorganisms

Extension: relating absorbance of light to density of yeast cells in culture:

Initial yeast population density calculation?

A

Initial yeast population density = average cell count in 1 field of view / volume

Yasmin Biology flashcards (3 decks)

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