Unit 6 Collumn 1 Flashcards
Transformation Experiment
an experiment that demonstrates the process of bacteria taking up and incorporating external genetic material (DNA) from their environment
Plasmid
a small
Hershey and Chase Experiment
Bacteriophage experiment. Proteins and DNA were tagged with a radioisotope and tracked as a virus attacked a bacteria. DNA entered cell
Chargaff Rule
A-T
Purine
A and G
Pyrimidine
T and C
Sugar Phosphate Backbone
a chain of alternating sugar and phosphate groups that forms the structural framework of DNA and RNA
Double Helix
twisting shape of DNA
Hydrogen Bond
bond due to polar charges
DNA Replication
process of copying DNA during S phase for Mitosis and Meiosis
Semiconservative Replication
the process by which DNA is copied to create two new DNA strands
Leading Strand
a strand of new DNA that is synthesized continuously and is complementary to the parental DNA strand
Lagging Strand
The lagging strand is synthesized in small fragments called Okazaki fragments.The lagging strand is synthesized discontinuously because the two strands of DNA run in opposite directions.
Topoisomerase
Flattens out DNA for replication
Helicase
“unzips” DNA by breaking hydrogen bonds between nitrogenous bases
SSB
keeps DNA separated after helicase separates the bases
RNA Primase
creates RNA primer so DNA polymerase III can attach
DNA Polymerase III
creates the complimentary strand of DNA to the original template strand
DNA Polymerase I
replaces the RNA primers with DNA
Ligase
connects the phosphate-sugar backbone at the end of replication
Primer
complementary section of RNA that provides an attachment point for DNA polymerase III
Okazaki Fragment
sections of complementary DNA created for the lagging strand during DNA replication
Protein Synthesis
creation of proteins
Transcription
complementary strand of mRNA created off of a template DNA strand for a gene
Translation
protein created from ribosome reading mRNA
DNA
deoxyribonucleic acid
RNA
ribonucleic acid
Nucleotide
monomer for Nucleic acids
Amino Acid
monomer for proteins
R
Group - 20 different possibilities that are the functional component of the amino acid
Ribose
sugar for RNA
Deoxyribose
sugar for DNA
Phosphate
phosphorus and 4 oxygens
Messenger RNA
carries the genetic information for a gene from nucleus to the ribosomes. Created as a complementary strand of RNA from a DNA gene template strand
Transfer RNA
tRNA has an anti-codon that is complementary to the mRNA codon. An amino acid is attached to the opposite end of the anticodon on the tRNA. The Amino acid is specific to the anticodon.
Ribosomal RNA
RNA component of the ribosome
Template Strand
original strand that the complementary strand is built/paired with.
Complementary Strand
new strand that is built/paired with the original template strand
Ribosome
site of protein synthesis. A site
RNA Polymerase
builds the mRNA from the template DNA strand
Central Dogma
a theory that describes how genetic information flows from DNA to RNA to protein.
Codon
3 letter base sequence on mRNA that holds the information for what amino acid is needed in the gene sequence
Anticodon
3 letter tRNA complement that ensures the correct amino acid is brought to the ribosome to correlate to the mRNA codon.
Redundancy
multiple codons per amino acids to allow for mistakes to happen but still code for the same amino acid
Initiation
start of transcription at the promoter region by RNA polymerase
Elongation
building of the complementary mRNA strand from the original DNA template strand by adding one nucleotide on at a time
Termination
release of mRNA from RNA polymerase
Spliceosome
helps to remove introns from mRNA in finishing process
MicroRNA/siRNA
small codes of RNA that are important for gene regulation. Death tags for mRNA to limit lifespan of mRNA
Wobble Hypothesis
a theory that explains how a single amino acid can be coded for by multiple codons.
tRNA synthetase
attaches correct amino acid to tRNA to correlate with the appropriate anticodon
Chaperone Protein
a diverse group of proteins that play crucial roles in the proper folding
Start Codon
tells the ribosome where to attach to the mRNA and start producing the protein in translation
Stop Codon
tells the ribosome when to stop translation and release the protein and mRNA