OHio Flashcards
Amino Acid
monomer for proteins, amino group - carbon - carboxyl group with a R-group off center carbon. 20 different amino acids
Anticodon
3 letter tRNA complement that ensures the correct amino acid is brought to the ribosome to correlate to the mRNA codon.
Central Dogma
a theory that describes how genetic information flows from DNA to RNA to protein.
Chaperone Protein
a diverse group of proteins that play crucial roles in the proper folding, assembly, and transport of other proteins in cells
Chargaff Rule
A-T, C-G; same amounts of A as T, and same amount of C as G.
Codon
3 letter base sequence on mRNA that holds the information for what amino acid is needed in the gene sequence
Complementary Strand
new strand that is built/paired with the original template strand
Deoxyribose
sugar for DNA
DNA
deoxyribonucleic acid
DNA Polymerase I
replaces the RNA primers with DNA
DNA Polymerase III
creates the complimentary strand of DNA to the original template strand
DNA Replication
process of copying DNA during S phase for Mitosis and Meiosis
Double Helix
twisting shape of DNA
Elongation
building of the complementary mRNA strand from the original DNA template strand by adding one nucleotide on at a time
Helicase
“unzips” DNA by breaking hydrogen bonds between nitrogenous bases
Hershey and Chase Experiment
Bacteriophage experiment. Proteins and DNA were tagged with a radioisotope and tracked as a virus attacked a bacteria. DNA entered cell, protein stayed out and washed away showing DNA was the component that carried the viral information.
Hydrogen Bond
bond due to polar charges, attracts nucleic bases together
Initiation
start of transcription at the promoter region by RNA polymerase
Lagging Strand
The lagging strand is synthesized in small fragments called Okazaki fragments.The lagging strand is synthesized discontinuously because the two strands of DNA run in opposite directions.
Leading Strand
a strand of new DNA that is synthesized continuously and is complementary to the parental DNA strand
Ligase
connects the phosphate-sugar backbone at the end of replication
Messenger RNA
carries the genetic information for a gene from nucleus to the ribosomes. Created as a complementary strand of RNA from a DNA gene template strand
MicroRNA/siRNA
small codes of RNA that are important for gene regulation. Death tags for mRNA to limit lifespan of mRNA
Nucleotide
monomer for Nucleic acids, Phosphate group - Sugar - and nitrogenous base
Okazaki Fragment
sections of complementary DNA created for the lagging strand during DNA replication
Phosphate
phosphorus and 4 oxygens, component of nucleotide, part of DNA/RNA backbone
Plasmid
a small, circular DNA molecule that can replicate independently from a cell’s chromosomal DNA. Can hold important DNA and be passed from one bacteria to another.
Primer
complementary section of RNA that provides an attachment point for DNA polymerase III
Protein Synthesis
creation of proteins
Purine
A and G, Double ringed nucleic bases
Pyrimidine
T and C, single ringed nucleic bases
R
Group - 20 different possibilities that are the functional component of the amino acid. Functional Group
Redundancy
multiple codons per amino acids to allow for mistakes to happen but still code for the same amino acid
Ribose
sugar for RNA
Ribosomal RNA
RNA component of the ribosome
Ribosome
site of protein synthesis. A site, P site, E site
RNA
ribonucleic acid
RNA Polymerase
builds the mRNA from the template DNA strand
RNA Primase
creates RNA primer so DNA polymerase III can attach
Semiconservative Replication
the process by which DNA is copied to create two new DNA strands, each containing one old strand and one new strand
Spliceosome
helps to remove introns from mRNA in finishing process
SSB
keeps DNA separated after helicase separates the bases
Start Codon
tells the ribosome where to attach to the mRNA and start producing the protein in translation
Stop Codon
tells the ribosome when to stop translation and release the protein and mRNA
Sugar Phosphate Backbone
a chain of alternating sugar and phosphate groups that forms the structural framework of DNA and RNA
Template Strand
original strand that the complementary strand is built/paired with.
Termination
release of mRNA from RNA polymerase
Topoisomerase
Flattens out DNA for replication
Transcription
complementary strand of mRNA created off of a template DNA strand for a gene
Transfer RNA
tRNA has an anti-codon that is complementary to the mRNA codon. An amino acid is attached to the opposite end of the anticodon on the tRNA. The Amino acid is specific to the anticodon.
Transformation Experiment
an experiment that demonstrates the process of bacteria taking up and incorporating external genetic material (DNA) from their environment, essentially changing their characteristics. Good bacteria mixed with dead lethal bacteria turned into lethal bacteria by taking in a component (later we find out it is DNA) of the lethal bacteria.
Translation
protein created from ribosome reading mRNA, bringing in tRNA, and attaching associated amino acids together in order
tRNA synthetase
attaches correct amino acid to tRNA to correlate with the appropriate anticodon
Wobble Hypothesis
a theory that explains how a single amino acid can be coded for by multiple codons.