Unit 6: Cellular Control Flashcards
How do cells control gene expression, and why is this important in multicellular organisms?
Cells control gene expression to produce specific proteins only when needed, conserving resources.
• In multicellular organisms, gene expression differences allow cells with identical DNA to specialize into various types (e.g., muscle or nerve cells).
• Control of gene expression happens at multiple levels: transcriptional, post-transcriptional, translational, and post-translational.
What is transcriptional regulation and how does it affect gene expression?
• Transcriptional regulation involves controlling whether a gene is transcribed into mRNA.
• It is primarily controlled by transcription factors that bind to DNA, either promoting or inhibiting the binding of RNA polymerase to initiate transcription.
• This level of regulation is crucial for determining which genes are active in a cell.
What are transcription factors and how do they influence gene transcription?
Transcription factors are proteins that bind to specific DNA sequences to control gene transcription.
• Activators increase transcription by aiding RNA polymerase binding.
• Repressors inhibit transcription by blocking RNA polymerase.
• Through these proteins, cells can fine-tune gene expression levels for specific cellular needs.
Define an operon and its components, explaining its role in prokaryotic gene regulation.
An operon is a cluster of genes in prokaryotes controlled as a single unit, ensuring efficient regulation.
• Components include:
• Promoter: Site for RNA polymerase binding.
• Operator: Site for repressor binding, controlling access to structural genes.
• Structural Genes: Encode proteins needed for specific cellular functions.
• This coordinated system allows the cell to activate or deactivate multiple genes simultaneously.
Describe the lac operon in E. coli and how it regulates lactose metabolism.
The lac operon controls genes (lacZ, lacY, lacA) for lactose processing.
• Without Lactose: The lac repressor binds to the operator, blocking transcription.
• With Lactose: Lactose binds to the repressor, changing its shape so it can’t bind the operator, allowing transcription.
• This regulation conserves energy by producing lactose-metabolizing enzymes only when lactose is present.
Explain the role of the lac repressor and operator in the lac operon.
Lac Repressor: A protein that binds to the operator in the lac operon, preventing transcription when lactose is absent.
• Operator: A DNA segment where the repressor binds, controlling access of RNA polymerase to structural genes.
• When lactose is available, it binds to the repressor, causing it to detach from the operator, allowing gene transcription to proceed.
What is post-transcriptional regulation, and how does it modify mRNA?
Post-transcriptional regulation occurs after mRNA is synthesized from DNA.
• Modifications include splicing, adding a 5’ cap, a poly-A tail, and mRNA editing.
• These changes can affect mRNA stability, translation efficiency, and the eventual protein product, adding flexibility to gene expression.
What is mRNA splicing, and why is it important in eukaryotic cells?
• mRNA Splicing: The process of removing non-coding regions (introns) and joining coding regions (exons) in mRNA.
• Splicing creates mature mRNA that can be translated into protein.
• This allows a single gene to produce different protein products through alternative splicing, increasing protein diversity.
What is mRNA editing and how does it contribute to protein diversity?
• mRNA editing involves adding, deleting, or altering specific nucleotides in the mRNA sequence.
• This can change the amino acid sequence of the resulting protein, allowing different protein variants from the same gene.
• mRNA editing enables cells to adapt protein functions without altering the original DNA, enhancing flexibility.
Describe cAMP’s role in gene expression regulation at the post-translational level.
• cAMP (cyclic AMP) acts as a secondary messenger that regulates proteins after they are synthesized.
• It binds to and activates protein kinase A (PKA), which then modifies target proteins.
• Through these changes, cAMP influences various cellular processes, including metabolism and gene expression.
Explain how cAMP activates protein kinase A (PKA) and the effects on target proteins.
cAMP binds to the regulatory subunits of PKA, causing them to release the catalytic subunits.
• The catalytic subunits become active and phosphorylate target proteins, altering their function.
• Phosphorylation can activate or deactivate enzymes, impacting cellular pathways post-translationally.
Summarize the role of transcriptional, post-transcriptional, and post-translational regulation in controlling gene expression.
• Transcriptional: Regulates the initiation of mRNA synthesis via transcription factors and operons (e.g., lac operon in E. coli).
• Post-Transcriptional: Involves mRNA splicing, editing, and stability modifications to control the mRNA’s translation potential.
• Post-Translational: Modifies proteins after translation (e.g., phosphorylation by PKA), adjusting protein function and activity.
Why is multilevel regulation of gene expression important for cellular function?
• Multilevel regulation ensures precise control over which proteins are produced, when, and in what amounts.
• It allows cells to adapt to environmental changes, conserve energy, and maintain specific functions based on their type.
• Such regulation is vital for processes like cell differentiation, response to signals, and efficient resource management in both prokaryotic and eukaryotic organisms.
How do some genes control the development of body plans in organisms?
Body Plan Genes direct the overall structure of an organism, determining the positioning and shape of body parts.
• Genes like homeobox genes (in animals) and homeotic genes (in plants) play key roles by controlling patterns of development.
• These genes are highly conserved, meaning they are similar across many species, indicating their critical role in body structure formation.
What are homeobox genes and how do they influence development?
• Homeobox Genes: A group of regulatory genes with a DNA sequence (homeobox) that encodes transcription factors.
• These transcription factors bind to DNA and activate other genes that control body structure formation during development.
• Homeobox genes are essential for determining the positioning of limbs, organs, and other body parts, ensuring a correct body plan.
What role does apoptosis play in the development of body plans?
Apoptosis is programmed cell death, crucial for normal development by removing cells no longer needed.
• It shapes structures (e.g., removing webbing between fingers) and removes damaged or unneeded cells to prevent potential harm.
• During development, apoptosis helps refine body parts by breaking down specific cells, contributing to organism shape and function.
Describe the process of apoptosis and the steps involved.
Apoptosis is a controlled sequence of events:
1. Cells shrink and their contents break down.
2. DNA and proteins degrade, and the cell membrane starts to bleb (form bubble-like protrusions).
3. Cell components are enclosed in vesicles for phagocytosis (cell “eating”).
• This efficient breakdown prevents inflammation and damage to surrounding tissues, allowing clean removal of unnecessary cells.
How does apoptosis help maintain balance in cell populations?
Apoptosis controls cell numbers by balancing cell production (mitosis) and cell death.
• Cells die via apoptosis as needed, preventing overgrowth, managing tissue shape, and removing potentially harmful cells.
• It is essential for processes such as immune system regulation, tissue renewal, and embryonic development.
Explain the connection between apoptosis and the cell cycle, and how they respond to external stimuli.
Genes that regulate apoptosis and cell cycle progression respond to signals like DNA damage or stress.
• When DNA is damaged, cells may initiate apoptosis to prevent the spread of mutations.
• External stimuli, such as infection or cellular stress, can trigger apoptosis as a protective mechanism to eliminate compromised cells.
What are mutations and how do they affect DNA?
Mutations are changes to the DNA base sequence, altering the genetic code.
• Types include point mutations (change in one base) and frameshift mutations (insertion or deletion, altering reading frame).
• Mutations can occur naturally during DNA replication or from external factors (e.g., radiation, chemicals).
What types of mutations can occur, and how do they affect genetic information?
Point Mutation: A single base change that may alter one amino acid in the protein.
• Frameshift Mutation: Insertions or deletions shift the reading frame, often changing multiple amino acids and resulting in a dysfunctional protein.
• Silent Mutation: A mutation that does not affect the protein’s amino acid sequence due to redundancy in the genetic code.
How can mutations be classified based on their effects on the organism?
Mutations can be neutral, beneficial, or harmful.
• Neutral: Have no effect on protein function or organism fitness.
• Beneficial: Improve the organism’s ability to survive or reproduce.
• Harmful: Lead to malfunctions in proteins, potentially causing diseases or developmental issues.
What is a neutral mutation, and why doesn’t it impact an organism’s phenotype?
Neutral Mutation: A change in DNA that doesn’t alter the protein or affect its function.
• Often occurs in non-coding regions of DNA or involves synonymous codons that produce the same amino acid.
• Neutral mutations do not impact the organism’s traits or fitness, so they are usually silent in the phenotype.
Provide an example of a beneficial mutation and how it aids survival.
An example is the sickle cell mutation, which provides resistance to malaria in carriers (heterozygous individuals).
• This mutation affects hemoglobin shape in red blood cells, creating an advantage in malaria-endemic areas.
• Beneficial mutations like this one can be naturally selected for, as they increase the organism’s chances of survival in specific environments.
What are harmful mutations and how can they lead to disease?
Harmful mutations result in dysfunctional proteins, potentially disrupting biological processes.
• Example: Cystic fibrosis caused by mutations in the CFTR gene, leading to defective chloride channels and mucus buildup.
• Such mutations can impair health, lead to genetic diseases, or disrupt normal development.
How can mutations influence gene expression and lead to developmental abnormalities?
Mutations can impact regulatory regions, affecting when, where, or how much a gene is expressed.
• Misregulation of body plan genes (e.g., homeobox genes) due to mutations can cause malformations or abnormal development patterns.
• For instance, incorrect activation of genes controlling limb formation may lead to duplicated or missing limbs.
