UNIT 6-8 PRACS Flashcards

1
Q

Counting chamber are seen under what miscroscope lens

A

SCANNING OBJ

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2
Q

The entirety

A

primary squares

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3
Q

Also called secondary corner
squares;

○ When viewing one secondary
square, we then switch to LPO.

A

secondary squares

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4
Q

squares in the middle
portion

A

Tertiary squares

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5
Q

smaller squares in
the middle portion

A

small central squares

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6
Q

THICKER AND SHORTER than WBC

A

RBC THOMA

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7
Q

With graduations: 0.5, 1, 101

A

RBC

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8
Q

LONGER BUT THINNER than RBC

A

WBC

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9
Q

With graduations: 0.5, 1, 11

A

WBC

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10
Q

Has larger bore

A

WBC

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11
Q

1.) Thick cover slip

2.) Suction device

A

ACCESSORY DEVICES

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12
Q

volume of bulb: RBC pipette

A

100 UNITS

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13
Q

volume of bulb of WBC

A

10 units

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14
Q

Bore size for the RBC is smaller, for WBC is larger.

A

TRUE

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15
Q

RBC OVERALL CAPACITY

A

101 UNITS

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16
Q

WBC OVERALL CAPACITY

A

10 UNITS

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17
Q

HOW MANY PARALLEL PLATFORMS SA IMPROVED NEUBAUER?

A

3 parallel platforms

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18
Q

1 primary square is equal to

A

3 x 3mm (9sqmm)

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19
Q

4 corner secondary squares is equal to

A

0.25 x 0.25 mm

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20
Q

central secondary square is equal to

A

0.2 x 0.2 mm

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21
Q

how many tertiary squares for RBC count for IMPROVED NEUBAUER

A

5 of the 25 tertiary squares

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22
Q

how many tertiary squares for WBC count in IMPROVED NEUBAUER

A

16 tertiary squares

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23
Q

how many tertiary squares for RBC count for NEUBAUER

A

5 of the 16 tertiary squares

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24
Q

The Fuchs-Rosenthal counting

grid has an area of?

and a depth of?

A

4 mm x 4mm

depth 0.2mm

25
Q

depth of speirs levy

A

0.2mm

26
Q

The area is arranged
into 2 horizontal
rows of 5 squares.

A

speirs-levy

27
Q

total area of speirs levy

A

1x1mm (total area of 10sq mm)

28
Q

used in counting the cellular elements
of the blood.

A

HEMACYTOMETER

29
Q

RBC has smaller bore compared to WBC pipette. TRUE OR FALSE?

A

TRUE

30
Q

MUST BE A HYPOTONIC SOLUTION

A

TRUE- WBC DILUTING FLUID

31
Q

how many drops should be discarded in WBC thoma

A

2-3 drops

32
Q

dispensing using thoma pipette in the
neubauer counting chamber.

A

Charging

33
Q

locate the ruled area for WBC counting by focusing the microscope using?

A

LPO

34
Q

INVERTED L-shape METHOD

A

WBC

35
Q

Count the WBCs seen on the 4 secondary squares
designated as W squares with an area of ?

A

4 mm2

36
Q

Record the count on each of the 4 secondary squares making sure that the cell difference between the squares is ?

A

12 or less

37
Q

Normal Range: 4,500 – 11,500 WBCs / mm³

A

WBC

38
Q

4 𝑚𝑚² 𝑋 1/10 𝑚𝑚 𝑋 1/20

A

divide mo total number of cells counted

WBC

39
Q

Nature sample
Operator’s error
Due to problematic equipment
Internal / field errors

A

sources of errors

40
Q

The number of erythrocytes contained in 1 liter of
blood is

A

erythrocyte number concentration.

41
Q

what is the Characteristic of a good RBC
diluting fluid?

A

ISOTONIC

42
Q

Best RBC Diluting Fluid
(cell shape is not
altered)

Can be stored for long
periods of time

A

DACIE’s/ Formol citrate

43
Q

composition of formol citrate

A

40% solution of formaldehyde = 10.0mL
3% W/V trisodium citrate = 990.0mL

44
Q

Not much recommended

● No corrosive effect
● Allows growth of
Yeast
● Produces clumping of
cells

A

HAYEMS

45
Q

Prevents Rouleaux formation

Precipitates protein in
hemoglobinemia and
hyperglobulinemia.

A

GOWERS

46
Q

Supports growth of
fungi

● High specific gravity
● Can stain WBC

A

TOISSON’s

47
Q

Used in emergency
cases and excessive
rouleaux formation
and auto-agglutinated
cells.

A

NSS

48
Q

how many drops should be discarded in RBC Thoma?

A

5-6 drops

49
Q

angle of the pipette while charging in RBC

A

30-35 degrees

50
Q

RBCs that touch any of the lines on the ________
borders, even if they are outside the tertiary square, are included.

A

TOP AND LEFT

51
Q

Record the count on each of the 5 tertiary squares
making sure that the cell difference between two
squares is

A

20 or less

52
Q

All counts must be done in duplicate. TRUE OR FALSE?

A

TRUE

53
Q

REFERENCE RANGE OF RBC

A

4.5-6.0x10¹²/L

54
Q

Errors due to materials/equipment
Operator’s technical errors
Errors due to nature of sample

A

Sources of Error in RBC Counting

55
Q

reference range for female for RBC

A

4.0-5.5 M/cumm

56
Q

reference range for preganants

A

3.0-5.0 M/cumm

57
Q

reference range for at birth

A

At birth = 7.0 M/cumm

58
Q

Convenient;
○ Employs the use of pre-measured volume of
diluting fluid in a plastic reservoir;
○ Specific amount of blood is collected.

A

unopette system