unit 5 tests

Question 1

what is ELISA?

Answer 1

a technique which uses antibodies and colour change to identify a concentration of an antigen in a mixture

Question 2

method of ELISA?

Answer 2

1. antigen from sample are attached to surface
2. a solution of a non-reaction protein is added to plate to block any unbound sites to prevent non-specific binding of the antibody
3. a specific antibody is applied to surface so it can bind to antigen
4. a substance containing the enzyme’s substrate is added
5. subsequent reaction produces a detectable signal higher concentration of antibody