Unit 4 - Lesson 10: Yeast & Beer Flashcards

1
Q

What does biotechnology use to make useful products?

A

Microorganisms

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2
Q

What process is often involved in biotechnology?

A

Fermentation, which uses the respiration of microorganisms to make products.

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3
Q

What is yeast used for regarding bread?

A

Yeast is often used to make bread rise.

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4
Q

Is yeast a single celled fungus or a multicellular fungus?

A

Yeast is a single celled fungus.

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5
Q

How does yeast achieve/absorb nutrients?

A

It uses saprotrophic nutrition to secrete extracellular digestive enzymes, which breakdown organic matter (normally dead or decaying). It then absorbs the nutrients.

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6
Q

(a) What are the products when yeast mixes with sugar?
(b) Write the chemical equation.

A

(a) When mixed with sugar, yeast produces carbon dioxide and water by aerobic respiration.
(b) C6H12O6 + O2 –> H2O + ATP

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7
Q

Where does the CO2 build up in the bread and what effect does this have on the bread?

A

CO2 builds up in the bread creating pockets, which makes the bread rise and have a spongy texture.

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8
Q

What does leavened bread mean?

A

Leavened bread (with yeast)

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9
Q

What does unleavened bread mean?

A

Unleavened bread (without yeast)

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10
Q

If sugar is not mixed with yeast, can aerobic respiration still occur? If so, how?

A

Yes. The glucose is obtained from the starch in the flour. The flour contains enzymes to break down starch to maltose. The extracellular digestive enzymes from the yeast convert this to glucose for respiration.

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11
Q

When the oxygen runs out, how does the yeast respire?

A

The yeast respires anaerobically.

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12
Q

When yeast respires anaerobically (without oxygen), what does it produce?

A

Ethanol (a type of alcohol).

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13
Q

Give the word equation for what glucose produces when yeast respires anaerobically.

A

Glucose –> Ethanol + Carbon Dioxide + Energy

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14
Q

When the bread is baked, the temperature rises and does what to the yeast? What process stops due to this? How is ethanol affected by this?

A

The temperature kills the yeast, so FERMENTATION stops. The alcohol is evaporated away.

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15
Q

When the yeast dies, what happens to the bread?

A

The bread stops rising but the air pockets remain.

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16
Q

What is the 3 step method for making bread?

A
  1. Mix yeast with flour and water. Adding sugar is useful to provide the sugar for respiration. Without it, enzymes in the flour breakdown the carbohydrates to sugars.
  2. Knead the dough and leave in a warm place to rise.
  3. Bake in the oven.
17
Q

How is wine made? What process?

A

Wine is made by the fermentation of grape juice sugars.

18
Q

What is used to ferment the sugars when making wine?

19
Q

What is wine made in and why?

A

Wine is made in large, airtight containers. This maintains the anaerobic conditions needed to produce the ethanol.

20
Q

What will happen if oxygen enters the airtight container when making wine?

A

If oxygen enters, the yeast will switch to aerobic respiration and ethanol production will stop.

21
Q

As the alcohol levels increase, when making wine, what happens to the yeast? What process is effected by this and how?

A

The yeast is killed. At this point, fermentation will stop.

22
Q

How is beer made? What process?

A

Beer is produced from the fermentation of barley.

23
Q

What kind of biomolecule is barley?

A

Barley is a type of carbohydrate called ‘starch’ (polysaccharide).

24
Q

What must starch be broken down into for respiration (when making beer)?

A

It must be broken down to a simple sugar for respiration.

25
Instead of having to break down starch into simple sugars for respiration when making beer, what can we use instead of regular starch?
To get around this, germinated barley seeds are used.
26
What is produced during germination? What does this product do to the starch?
Amylase is produced. This breaks down the starch to maltose.
27
What does yeast secrete to break the maltose down to glucose for respiration?
Yeast secretes extracellular maltase to break the maltose down to glucose for respiration.
28
What is barley fermented in?
Large vats.
29
A thick, frothy layer on the surface (when making beer) signifies what? What has built up to create this thick, frothy layer on the surface?
As CARBON DIOXIDE builds up, it creates a thick frothy layer on the surface, maintaining anaerobic conditions beneath.
30
FILLER CARD - MOVING ONTO CORE PRACTICAL
FILLER CARD - MOVING ONTO CORE PRACTICAL
31
Practical: Investigate The Respiration Rate Of Yeast What apparatus is needed?
- 2 Test Tubes - 1 Bung attached to a tube - Yeast - Sugar - Distilled Boiled Water - Liquid Paraffin OR Oil - Pipette - Lime water OR hydrogen-carbonate indicator solution
32
Practical: Investigate The Respiration Rate Of Yeast What is the 7 step method for this practical?
1. Gently boil a little of the distilled water to remove any dissolved oxygen. Let it cool. 2. Mix the yeast, sugar & boiled distilled water in a test tube. 3. Using a pipette, gently add a thin layer of liquid paraffin or oil to the surface of the yeast mixture. This will prevent oxygen that's inside the test tube from entering the mixture. 4. Attach a bung with a test tube leading to the second test tube filled with the remaining water/limewater/hydrogen carbonate indicator solution. For more precise results, the tube could lead to a gas syringe (this would show us the volume of CO2 produced). 5. Repeat steps 1-4 to create a second set of apparatus. This will be your control experiment. The only difference will be to use boiled (killed) yeast. 6. Place the tube with the yeast mixture in a water bath set to a certain temperature. 7. Once the mixture has had time to adjust to the new temperature, calculate the rate of carbon dioxide production by dividing the number of bubbles produced by the time taken to do so. If limewater, measure the time it takes to turn cloudy. If hydrogen-carbonate solution is used, measure the time it takes to turn from orange to yellow. Compare the results of the test experiment and the control experiment.
33
Modify the practical to investigate the effect of temperature on respiration rate.
Carry out the experiment multiple times at different temperatures.
34
Modify the practical to investigate the effect of using different sugars on respiration rate.
Carry out the experiment multiple times using different sugar types, i.e. glucose, maltose, fructose, lactose & sucrose.
35
Modify the practical to investigate the effect of sugar concentration on respiration rate.
Carry out the experiment multiple times using different concentrations of sugar.