Unit 4: DNA and Protein Synthesis Flashcards
What does DNA stand for
Deoxyribonucleic Acid
What is the monomer for DNA
nucleotide
What is the circle part of a nucleotide
phosphate
What makes up the spine of a DNA strand
phosphate and sugar
what bonds bases together
weak hydrogen bonds
What rule states that if there are 5 adenine there must be 5 thymine?
Chargaff’s rule.
Chargaff’s rule
complementary bases will always bond with eachother and they are equal in quantity
complimentary means?
nitrogenous bases that bind together (they fit like 2 puzzle pieces)
compare and contrast the sugar of DNA and RNA
DNA:Deoxyribose(no oxygen sugars) RNA: Ribose
mRNA bases
adenine+uracil, cytosine+gunaine
DNA bases
adenine+ thymine, cytosine+guanine
base pairs
2 bases that always bond with eachother
Describe the shape and structure of DNA
double helix, the backbones are antiparallel so they are parallel but opposite. hydrogen bonds bind the complimentary bases. Sugar and a phosphate form the backbones.
Location of DNA
Nucleus
Location of RNA
moves from nucleus to cytoplasm in replication
Replication happens in what stage of mitosis?
interphase
Describe the chromosomes after replication
each cell gets a complete set of chromosomes
1st step in replication
Chemical bonds split between base pairs. DNA unzips
2nd step in replication
free nucleotide bases pair up with complementary base on DNA stands
3rd step in replication
Sugars and phosphates bond between free nucleotides
4th step in replication
2 identical strands of DNA (consists of one old strand and one new strand)
semi-conservative definition
one half of the strand is old, one Half is new
(transcription)DNA helicase does what in what step and is what type of macromolecule?
a protein that unzips DNA (1st step)
(transcription)DNA polymerase does what in what step and what type of macromolecule?
An enzyme that adds bases (2nd step)
(transcription)Ligase does what in what step?
mRNA is created Ligase rezips DNA molecules (3rd step)
(transcription)location of mRNA
nucleus to cytoplasm
(translation) location of mRNA
ribosome
(translation)steps
(1)ribosome attaches to mRNA (2)tRNA with an amino acid tries to attatch to a codon that is complementary to it’s anticodons (3)ribosome slides to the next codon (4) new tRNA bonds with the second codon (5) amino acid from the first tRNA attatches to the new amino acid(tRNA goes to pick up new amino acids when it’s empty) (6)ribosome contintues this process until stop codon ireached to make a squence of amino acids (a protein)
Different types of RNA and their purpose in translation
mRNA(messenger RNA, created during transcription.) tRNA (carries enzymes that later form proteins) rRNA (aka:ribosome, slides the strand of mRNA every codon so tRNA can make sure the anticodons are complimentary)
what is a codon
3 bases in mRNA that code 1 enzyme
what is an anticodon
3 bases in tRNA
what is a mutation
a mistake in genetic coding
what is a mutagen
a substance or reason that causes a mutation
mutagen examples
chemicals, radiation, high temps
what mutation is a change in a base pair. One replaces another
substitution/ point mutation
what is a mutation that inserts or delete a base
frame shift mutation
main steps of mitosis
prophase, metaphase, anaphase, telophase
what is interphase
(in between stage of cell division(mitosis))
what is prophase
the dna forms into chromosomes from it’s original noodle appearance. (nucleus dissolves)
what is metaphase
the chromosomes line up in the middle of the cell
what is anaphase
the chromosomes are being pulled apart
what is telophase
the chromosomes are carefully placed into the new forming nucleus of each daughter cell
what is cytokinesis
splitting cells from eachother at the end
what type of cells do mitosis create
body (somatic)
what is in common in parent cells and daughter cells in mitosis
everything, they’re identical
what is a chromosome
condensed DNA
how many chromosomes are in each daughter cell
the same amount as the parent cell
what are the reasons for mitosis
growth and repair
what type of reproduction is mitosis
asexual
what is the center of a chromosome called
centromere
what is a chromosome before DNA replication called?
haploid
what is a chromosome after DNA replication called
diploid
What are the phases of interphase and what do they do?
G1 (cell growth) S (DNA replication) G2 (Preparation for mitosis)
what stage does DNA start dissolving in mitosis
prophase
what stage does DNA start reforming in mitosis
telophase
what is surface area in a cell
cell membrane
what is volume in a cell
cytoplasm
what size of a cell is most efficient at homeostasis? why?
small, waste and nutrients are faster at leaving and entering the cell because there is less distance
what becomes larger as cell size increases
volume becomes larger than surface area
what size of cube has a larger surface are to volume ratio?
small
is a high or low surface are to volume ratio more efficient in homeostasis?
high. (6:1>8:1)