Unit 4- Cellular Respiration & Photosynthesis Flashcards
Where does energy come from in the cells?
Chemical Bonds or Chemical Gradients that store potential energy or from the sun
Everything cells do requires _____
Energy
Much og stored chemical energy comes in the form of ____, particularly_____
Sugars ; Glucose
Define Cellular Respiration
A process whereby cells harvest energy from food molecules, usually by breaking fown sugar to CO2 and H2O
What are the molecules that usually provide cells with energy for cellular respiration?
Glucose, Fatty Acids, Proteins
Define: Activated Carriers
Portable sources of energy that fuel Biosyntesis (ATP, NADH)
Energy released from high-energy bonds of sugars is often captured in other high energy chemical bonds of what molecules?
Activated Carriers
What type of process is Cellular Respiration (Anabolic/Catabolic)
Catabolic (breaking down molecules)
Cellular Respiration is a _____ Process; It occurs in a series of small reactions.
Stepwise
What is a benefit of Cellular Respiration being a Stepwise process?
It allows cells to harvest energy at many steps while the food molecule is being degraded.
What are the two ways Animal Cells make ATP?
Substrate-Level Phosphorylation and Oxidative Phosphorylation
Define: Substrate-Level Phosphorylation
Breakdown of one substrate of enzyme powers that enxyme to add inorganic Phosphate to ADP (Makes ATP)
Define: Oxidative Phosphorylation
Production of ATP powered by the Electron-Transport System
When do both of the two ways cells make ATP occur?
During Cellular Respiration
Define: Catabolism
Process of breaking down food molecules into smaller molecules, releasing energy to generate activated carriers.
What are the 3 major steps of Catabolism?
Digestion
Generation of Acetyl CoA
Complete Oxidation of Acetyl CoA
Where does digestion take place?
Outside cells (Lumen of intestine) or in lysosomes
How are larger molecules broken down?
Into constituent building blocks (amino acids, fatty acids, sugars, etc.)
Where does the digestion continue after the Lumen of the intestines
The building blocks enter the cytosol for the next step.
Where does the generation of Acetyl CoA occur?
It partially occurs in the cytosol/matrix
How many pyruvates are generated during Glycolysis?
2 Pyruvates for 1 Glucose Molecule
Each pyruvate enters the Mitochondrial Matrix where it’s converted to what Molecule?
Acetyl CoA
How are fatty acids broken down?
They are broken down two Carbons at a time
How many acetyl CoA molecules are generated from each carbon of a fatty acid?
1 Acetyl CoA is generated for every two carbons
True or false: breakdown of Glucose and Fatty Acids both generate high energy Electron carriers?
TRUE
Where does the complete oxidation of Acetyl CoA occur?
In the Mitochondria
What cycle do the Acetyl CoA molecules enter?
The Citric Acid Cycle
NADH molecules donate their electrons to _______ which harnesses the electron’s energy to drive Oxidative Phosphorylation to produce ATP
The Electron Transport Chain
What does the NADH to ETC transfer consume?
Oxygen (O2)
Where does Glycolysis occur?
In the Cytosol
Glycolysis produces _____ in the absence of ____
ATP; O2
One Glucose molecule produces what in glycolysis?
2 Pyruvates
2 Net ATPs
2 NADH
True or false: Glycolysis is a new or derived trait used for generating ATP?
False: This is likely an ancient process.
What is G3P
Glyceraldehyde 3-Phosphate
Energy is relased from the electrons as they fall down the _____ to make _____.
ETC; ATP
Once NADH delivers the electrons to the___, it becomes ____ again, supplying NAD+ to meet the demands of ____ in the ______
NAD+; Glycolysis; Cytosol
What happens if NAD+ is not regenerated?
Respiration will Cease.
What is fermentation?
The process that allows glycolysis to continue in the absence of O2 by recycling cytoplasmic NADH back to NAD+ so that a small amount of ATP can be produced.
True or False: In the absence of O2 and fermentation occurring, other respiratory pathways can still function.
False: all other respiratory pathways are shut down in
absence of O2
What would occur if cytoplasmic NAD+ was not recycled for fermentation to occur?
No ATP would be produced, resulting in the organism’s death.
In fermentation, where do Pyruvate and NADH stay?
In the Cytosol
During fermentation, electrons from NADH are put back onto _____ or a break-dowhn product of it.
Pyruvate
The direct addition of Electrons tp puruvate produces?
Lactic Acid/Lactate
An Addition of electrons to pyruvate after it has lost one Carbon in the form of CO2 produces what?
Ethanol (Alcohol Fermentation)
Lactic Acid fermentation and Alcohol Fermentation both result in what?
Regeneration of NAD+ so Glycolysis can continue
Several small organic molecules are coverten to Acetyl CoA in the mitochondria, including _____ and _____
Pyruvate and Fatty Acids
What are the 3 Reactions that the Pyruvate Dehydrogenase Complex performs?
Decarboxylation (Removal of Carboxyl group in form of CO2
Formation of NADH using NAD+ and Electrons from Pyruvate
Generation of Acetyl Coa (Addition of CoA)
Fatty Acids are first activated by ___ to CoA, then trimmed 2__ at a time, with each two ___ eventually forming ______
Linkage
Carbons
Carbons
Acetyl CoA
NADH and FADH2 are generated for every _____ formed
Acetyl CoA
Where does generation of Acetyl CoA occur?
In the Mitochondrial Matrix
What is Acetyl CoA generated from?
Sugars and fats. Acetyl CoA readily enters the Citric Acid Cycle.
Sugars are readily converted to glucose or intermediates of ______
Glycolysis
How are Amino Acids processed in the Citric Acid Cycle?
They can be converted into various intermediates of the Citric Acid Cycle in the Mitochondrial Matrix
Sugars, Fats, and Proteins can all feed into _____ Pathways
Catabolic
The Citric Acid Cycle Oxidizes _____
Acetyl Groups
The _____ from Acetyl CoA enter the Citric Acid Cycle
Acetyls
What is released as a waste product of Acetyl group Oxidation?
CO2 is released as a waste product
The Citric Acid Cycle Requires ____ Indirectly
O2
NAD+ must be regenerated, otherwise it would all be trapped in the form of _____.
NADH
If the NAD+ is trapped as NADH, what would occur?
NADH cannot accept Electrons from the Citric Acid Cycle, resulting in a halt of the cycle.
The Acetyl group is transferred from a CoA to a 4-C compound _____,
Oxaloacetate
Oxaloacetate transfer generates ______ _____ a 6-C compound
Citric Acid (Citrate)
By the end of the Citric acid Cycle, Oxaloacetate will be
Regenerated
The Citric Acid Cycle generates _ NaDH Molecule(s)
3
The Citric Acid Cycle generates _ FADH MOlecule(s)
1
The Citric Acid Cycle generates ___ GTP Molecule(s)
1
1 GTP Molecule is approximately equivalent to 1 ____ molecule
ATP
What product is produced as waste in the citric acid cycle? How many are produced?
CO2; 2 Molecules
Oxidative Phosophorylation
Electrons carried by NADH and FADH2 are delivered to the Electron Transport Chain (ETC)
What is the Electron Transport Chain?
A Series of electron carriers embedded in the inner mitochondrial membrane (Plasma Membrane of Prokaryotes)
AS Electrons are passed from carrier to carrier, energy is ____ in the Electron Transport Chain
Released
Electrons lose energy as they ____ the ETC.
Move down or Fall down the ETC.
Energy is used to pump ____ across the mitochondrial membrane into the intermembrane space, generating a ____ Gradient that can drive ATP Production
Protons (H+); Proton Gradient
How Many ATP are generated via the ETC?
~26-28 ATP
What does O2 do in the ETC?
O2 “pulls” electrons down the ETC, being eventually reduced to H2O
Cells must regulate energy _____/_____ in order to function when energy supplies are scarce
Usgage/Storage
What do the control mechanisms regulate in metabolism?
They regulate which pathways particular metabolites will enter
What are the key components of the control mechanisms in metabolism?
Enzyme regulation (Both Positive and Negative)
Gluconeogenesis
When fasting or undergoing intense physical exercise, glucose reserves cannot be replenished fast enough. Gluconeogenesis can function to replenish glucose stores
Gluconeogenesis is essentially the reverse of what process?
Glycolysis
What energy cost is associated with Gluconeogenesis?
4 ATP and 2 GTP
Gluconeogenesis is the formation of Glucose from what molecule?
2 Pyruvate molecules
How does the cell determine if it will undergo Glycolysis or Gluconeogenesis?
Enzyme regulation determines which process is favored.
Phosphofructokinase is involved in what process?
Gluconeogenesis
Phosphofructokinase is regulated ______by feedback mechanisms
Allosterically
Phosphofructokinase is activated by
ADP, AMP, and Pi(Inorganic Phosphate)
Phosphofructokinase is inhibited by
ATP
Fructose 1,6-Bisphosphate is involved in what process?
Glycolysis
Fructose 1,6-Bisphosphate Is _____ regulated by feedback mechanisms
Allosterically
Fructose 1,6-Bisphosphate is regulated in the opposite manner as ______
Phosphofructokinase
Fructose 1,6-Bisphosphate is activated by
ATP
Fructose 1,6-Bisphosphate is inhibited by
ADP, AMP, Pi (Inorganic Phosphate)
What is Glycogen?
A polymer of Glucose, storage from of Glucose in Animals
Where is Glycogen found in Animal Cells?
Found in all cells, but large stores are in liver and muscle cells.
Production of Glycogen is regulated according to ____
Need
If ATP demand is high, glycogen is ____ to ____
Broken Down; Glucose
What breaks down Glycogen into Glucose?
Glycogen Phosphorylase
If ATP demand is low, Glycogen is ____ from _____
Produced ; Glucose
What produces Glycogen from Glucose?
Glycogen Synthetase
Electron Transport is a key process of what important pathways?
Photosynthesis and Cellular Respiration
The Majority of APT is generated by ____ ____ ____ _____
Membrane-Based Oxidative Phosphorylation
True or False: Oxidative Phosphorylation could not occur without a membrane
TRUE
In oxidative phosphorylation, electron transport
along membrane facilitates
generation of______ gradient across that membrane
H+ (Proton)
Where does Oxidative Phosphorylation occur in Eukaryotes?
It occurs along the inner Mitochondrial Membrane, creating a high Proton concentration in the intermembrane space and a low Proton concentration in the Mitochondrial Matrix