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MDL 251 Microbiology > Unit 4 > Flashcards

Unit 4 Flashcards

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1
Q

The GasPak anaerobic system will remove _____, and replace it with _____ and _____.

A

Oxygen, Hydrogen and Carbon Dioxide

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2
Q

This oxidase positive, gram negative diplococci is not part of normal human flora and is spread person to person by sexual contact.

A

Neisseria gonorrhoeae

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3
Q

To determine if an organism is definitely an obligate anaerobe what procedure must be performed?

A

Aerotolerance test

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4
Q

This organism may cause lethal meningitis but is commonly carried in the respiratory tract of humans and may be asymptomatic.

A

Neisseria meningitdis

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5
Q

The candle jar will be useful when culturing organisms requiring

A

increased carbon dioxide

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6
Q

This slender anaerobic gram negative rod produces a fluorescent chartreuse color and is sensitive to colistin and kanamycin

A

Fusobacterium nucleatum

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7
Q 
All of the following medium are examples of PRAS media except:
Anaerobic blood agar
LKV agar
BBE agar
Modified Thayer Martin
A

Modified Thayer Martin

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8
Q

Among H. influenzae strains, group designations for serotyping are based on:

A

capsular characteristics

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9
Q

A colony was taken from growth on a chocolate plate from a respiratory specimen. The gram stain is gram negative diplococci. What is the next test that should be performed?

A

Oxidase

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10
Q

Which atmospheric condition is optimum for the cultivation of Campylobacter jejuni?

A

Microaerophilic

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11
Q

Proprionibacterium acnes will appear on gram stain as:

A

small club shaped gram pos rods

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12
Q

All of these are indicative of the presence of anaerobes, except:
Goul odor
Growth at the surface of liquid (thioglycollate) media
Gas
Positive gram stain with no aerobic growth

A

Growth at the surface of liquid (thioglycollate) media

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13
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Bile

A

Suitable

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14
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Throat swab

A

Unsuitable

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15
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Coughed (expectorated) sputum

A

Unsuitable

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16
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Pleural fluid

A

Suitable

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17
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Endometrium

A

Suitable

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18
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Blood

A

Suitable

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19
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Feces

A

Unsuitable

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20
Q

Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Aspirated bladder urine

A

Suitable

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21
Q

Match the organism with the appropriate term, disease or selective media.
CCFA agar

A

Clostridium difficile

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22
Q

Match the organism with the appropriate term, disease or selective media.
Gas gangrene

A

Clostridium perfringens

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23
Q

Match the organism with the appropriate term, disease or selective media.
Botox

A

Clostridium botulinum

24
Q

Match the organism with the appropriate term, disease or selective media.
Satellite test

A

Haemophilus influenzae

25
Q

Match the organism with the appropriate term, disease or selective media.
Modified Skirrow agar

A

Campylobacter jejuni

26
Q

Match the organism with the appropriate term, disease or selective media.
BBE agar

A

Bacteroides fragilis

27
Q

Match the organism with the appropriate term, disease or selective media.
Modified Thayer Martin

A

Neisseria gonorrhoeae

28
Q

Match the organism with the appropriate term, disease or selective media.
Bread crumb colonies

A

Fusobacterium nucleatum

29
Q

Match the organism with the appropriate term, disease or selective media.
Molar tooth colonies

A

Actinomyces sp.

30
Q

True or False?

A direct gram stain of endocervical secretions is diagnostic of the presence or absence of N. gonorrhoeae

A

False

31
Q

True or False?

Campylobacter jejuni, Moraxella catarrhalis and Neisseria meningitidis are all oxidase positive.

A

True

32
Q

Vaccination is responsible for the reduced incidence of severe invasive infections caused by:

A

Haemophilus influenzae

33
Q

The optimum temperature for the primary cultivation of campylobacter jejuni is:

A

42 degrees C

34
Q

A positive ALA test is useful for the identification of certain Haemophilus spp. by establishing the following:

A

X factor independence

35
Q

True or False?

All specimens received for anaerobic culture should be plated and incubated for 18-24 hours.

A

False

36
Q 
Infection with which of the following organisms is not associated with the ingestion of food products:
Haemophilus influenzae
Campylobacter jejuni
Clostridium botulinum
Clostridium perfringens
A

Haemophilus influenzae

37
Q

Performing the CLO test from a biopsy will cause a color change when this organism is detected:

A

Helicobacter pylori

38
Q

A typical gram stain of Haemophilus influenzae is best described as:

A

Gram negative coccobacilli

39
Q

Which plate should be used for the primary cultivation of Haemophilus influenzae?

A

Chocolate agar

40
Q 
Which of these anaerobes is part of the normal flora of teh skin and is rarely implicated in infections?
Bacteroides fragilis
Clostridium perfringens
Proprionibacterium sp.
Clostridium botulinum
A

Proprionibacterium sp.

41
Q

The development of Guillan-Barre syndrome is associated with infection with which organism?

A

Campylobacter jejuni

42
Q

This factor, present in several bacteria and yeast, is responsible for satellite growth of Haemophilus sp. around colonies of Staphylococcus species on sheep blood agar.

A

NAD

43
Q

This organism produces a powerful preformed toxin which may be ingested with food and lead to respiratory paralysis.

A

Clostridium botulinum

44
Q 
Which growth requirement is not appropriate for the fultivation of Haemophilus influenzae:
5-10% CO2
Microaerophilic environment
X and V factors
35-37 degree C incubation
A

Microaerophilic environment

45
Q

The proper specimen to collect when H. pylori is suspected is:

A

Gastric biopsy

46
Q 
Which of the following is a gram positive rod:
Haemophilus influenzae
Campylobacter jejuni
Moraxella catarrhalis
Clostridium difficile
A

Clostridium difficile

47
Q

This organism is known to be the major cause of bacterial meningitis worldwide among non-vaccinated children

A

Haemophilus influenzae

48
Q

When performing the ALA test, the detection of this end product will indicate factor independence.

A

Porphyrins

49
Q 
A CSF (cerebrospinal fluid) specimen from a 21 yo college student grew a pure culture of moist grey colonies. The following lab results were observed:
Gram stain = gram negative diplococci 
BAP and Chocolate agar = aerobic growth in CO2 incubator
MacConkey and PEA= no growth
Oxidase= positive 
DNase - neg 
Carbohydrate fermentation = glucose-pos, maltose–pos, sucrose–neg, lactose–neg
What is the most likely organism?
A

Neisseria meningiditis

50
Q

A diabetic patient presents to the emergency department with excrutiating foot pain. Upon further exam he is found to have a foul smelling wound at the base of his foot. A piece of tissue is submitted for aerobic and anerobic culture. The culture results are as follows.
No growth on Blood, Chocolate and Macconkey agar incubated at 35degrees in ambient air.
Growth on PRAS-ANA brucella plate inubated in a jar with a Gaspak
Growth in the bottom of a thioglycollate broth.
Gram stain=large gram positive (and gram variable) rods, no spores observed
PRAS-ANA brucella agar shows double zone of beta hemolysis

Special potency disks = Kanamycin-sensitive,
Vancomycin-sensitive, Colistin-resistant

Which organism is most likely the agent of his infection?

A

Clostridium perfringens

51
Q

A patient has developed renal symptoms. After a series of tests a liver abscess was found.
She had recently undergone surgery of the small bowel. The abscess was aspirated and sent to lab on swabs and in special anaerobic collection tubes.
The following culture results were observed.
Growth on ANA blood plate and LKV plate after 48 hour anaerobic incubation. (no hemolysis)
Subsequent culture of those colonies to an aerobic chocolate plate resulted in no growth.
Blackening was observed on BBE agar.
Special potency disks:
Kanamycin-resistant
Vancomycin-resistant
Colistin-resistant

What is the most likley ID?

A

Bacteroides fragilis

52
Q

A specimen was isolated from the cerebrospinal fluid of a 4 yr old girl visiting from outside the US. The following results were observed after 48 hr incubation in CO2.
Choc = small grey colonies with a mouse-like odor
Gram stain= gram negative coccobacilli
Blood agar= no growth
MacConkey = no growth
Satteliting with Staphylococcus = positive
ALA= neg

The most likely organism is:

A

Haemophilus influenzae

53
Q

A sputum specimen was obtained from a 79 year old male. He has COPD (chronic obstructive pulmonary disease). The xrays suggest pneumonia.
His culture results are as follows:
Growth on Chocolate and Blood agar: large opague colonies
Macconkey= no growth
Gram stain = gram negative diplococci
satellite test = negative
Oxidase=positive
Carbohydrate fermentation: Glucose=neg, Maltose=neg, Sucrose=neg, Lactose=neg
Dnase=positive
Beta lactamase=negative

A

Moraxella catarrhalis

54
Q 
Cultivation of H. ducreyi will require all of the following except:
4-7 day incubation
X Factor
V Factor
high humidity
A

V Factor

55
Q

Actinomyces is usually involved in infections of

A

Oral/facial area

56
Q

A post surgical drainage from an abdominal wound grew an anaerobic organism on a blood agar plate at 48 hours. It is not aerotolerant. The gram stain showed gram neg bacilli. The following reactions were observed:
Nitrate = neg
Catalase= negative
Growth on BBE = neg (no blackening)
Kanamycin = sensitive ,Vancomycin = resistant , Colistin= sensitive
The organism will likely be identified as:

A

Fusobacterium nucleatum

57
Q

The ingestion of honey is not recommended for young children because it may contain the spores of this anaerobic organism.

A

Clostridium botulinum

MDL 251 Microbiology (4 decks)

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