Unit 4 Flashcards
The GasPak anaerobic system will remove _____, and replace it with _____ and _____.
Oxygen, Hydrogen and Carbon Dioxide
This oxidase positive, gram negative diplococci is not part of normal human flora and is spread person to person by sexual contact.
Neisseria gonorrhoeae
To determine if an organism is definitely an obligate anaerobe what procedure must be performed?
Aerotolerance test
This organism may cause lethal meningitis but is commonly carried in the respiratory tract of humans and may be asymptomatic.
Neisseria meningitdis
The candle jar will be useful when culturing organisms requiring
increased carbon dioxide
This slender anaerobic gram negative rod produces a fluorescent chartreuse color and is sensitive to colistin and kanamycin
Fusobacterium nucleatum
All of the following medium are examples of PRAS media except: Anaerobic blood agar LKV agar BBE agar Modified Thayer Martin
Modified Thayer Martin
Among H. influenzae strains, group designations for serotyping are based on:
capsular characteristics
A colony was taken from growth on a chocolate plate from a respiratory specimen. The gram stain is gram negative diplococci. What is the next test that should be performed?
Oxidase
Which atmospheric condition is optimum for the cultivation of Campylobacter jejuni?
Microaerophilic
Proprionibacterium acnes will appear on gram stain as:
small club shaped gram pos rods
All of these are indicative of the presence of anaerobes, except:
Goul odor
Growth at the surface of liquid (thioglycollate) media
Gas
Positive gram stain with no aerobic growth
Growth at the surface of liquid (thioglycollate) media
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Bile
Suitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Throat swab
Unsuitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Coughed (expectorated) sputum
Unsuitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Pleural fluid
Suitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Endometrium
Suitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Blood
Suitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Feces
Unsuitable
Indicate whether the following clinical specimens are suitable or unsuitable for anaerobic culture:
Aspirated bladder urine
Suitable
Match the organism with the appropriate term, disease or selective media.
CCFA agar
Clostridium difficile
Match the organism with the appropriate term, disease or selective media.
Gas gangrene
Clostridium perfringens
Match the organism with the appropriate term, disease or selective media.
Botox
Clostridium botulinum
Match the organism with the appropriate term, disease or selective media.
Satellite test
Haemophilus influenzae
Match the organism with the appropriate term, disease or selective media.
Modified Skirrow agar
Campylobacter jejuni
Match the organism with the appropriate term, disease or selective media.
BBE agar
Bacteroides fragilis
Match the organism with the appropriate term, disease or selective media.
Modified Thayer Martin
Neisseria gonorrhoeae
Match the organism with the appropriate term, disease or selective media.
Bread crumb colonies
Fusobacterium nucleatum
Match the organism with the appropriate term, disease or selective media.
Molar tooth colonies
Actinomyces sp.
True or False?
A direct gram stain of endocervical secretions is diagnostic of the presence or absence of N. gonorrhoeae
False
True or False?
Campylobacter jejuni, Moraxella catarrhalis and Neisseria meningitidis are all oxidase positive.
True
Vaccination is responsible for the reduced incidence of severe invasive infections caused by:
Haemophilus influenzae
The optimum temperature for the primary cultivation of campylobacter jejuni is:
42 degrees C
A positive ALA test is useful for the identification of certain Haemophilus spp. by establishing the following:
X factor independence
True or False?
All specimens received for anaerobic culture should be plated and incubated for 18-24 hours.
False
Infection with which of the following organisms is not associated with the ingestion of food products: Haemophilus influenzae Campylobacter jejuni Clostridium botulinum Clostridium perfringens
Haemophilus influenzae
Performing the CLO test from a biopsy will cause a color change when this organism is detected:
Helicobacter pylori
A typical gram stain of Haemophilus influenzae is best described as:
Gram negative coccobacilli
Which plate should be used for the primary cultivation of Haemophilus influenzae?
Chocolate agar
Which of these anaerobes is part of the normal flora of teh skin and is rarely implicated in infections? Bacteroides fragilis Clostridium perfringens Proprionibacterium sp. Clostridium botulinum
Proprionibacterium sp.
The development of Guillan-Barre syndrome is associated with infection with which organism?
Campylobacter jejuni
This factor, present in several bacteria and yeast, is responsible for satellite growth of Haemophilus sp. around colonies of Staphylococcus species on sheep blood agar.
NAD
This organism produces a powerful preformed toxin which may be ingested with food and lead to respiratory paralysis.
Clostridium botulinum
Which growth requirement is not appropriate for the fultivation of Haemophilus influenzae: 5-10% CO2 Microaerophilic environment X and V factors 35-37 degree C incubation
Microaerophilic environment
The proper specimen to collect when H. pylori is suspected is:
Gastric biopsy
Which of the following is a gram positive rod: Haemophilus influenzae Campylobacter jejuni Moraxella catarrhalis Clostridium difficile
Clostridium difficile
This organism is known to be the major cause of bacterial meningitis worldwide among non-vaccinated children
Haemophilus influenzae
When performing the ALA test, the detection of this end product will indicate factor independence.
Porphyrins
A CSF (cerebrospinal fluid) specimen from a 21 yo college student grew a pure culture of moist grey colonies. The following lab results were observed: Gram stain = gram negative diplococci BAP and Chocolate agar = aerobic growth in CO2 incubator MacConkey and PEA= no growth Oxidase= positive DNase - neg Carbohydrate fermentation = glucose-pos, maltose–pos, sucrose–neg, lactose–neg What is the most likely organism?
Neisseria meningiditis
A diabetic patient presents to the emergency department with excrutiating foot pain. Upon further exam he is found to have a foul smelling wound at the base of his foot. A piece of tissue is submitted for aerobic and anerobic culture. The culture results are as follows.
No growth on Blood, Chocolate and Macconkey agar incubated at 35degrees in ambient air.
Growth on PRAS-ANA brucella plate inubated in a jar with a Gaspak
Growth in the bottom of a thioglycollate broth.
Gram stain=large gram positive (and gram variable) rods, no spores observed
PRAS-ANA brucella agar shows double zone of beta hemolysis
Special potency disks = Kanamycin-sensitive,
Vancomycin-sensitive, Colistin-resistant
Which organism is most likely the agent of his infection?
Clostridium perfringens
A patient has developed renal symptoms. After a series of tests a liver abscess was found.
She had recently undergone surgery of the small bowel. The abscess was aspirated and sent to lab on swabs and in special anaerobic collection tubes.
The following culture results were observed.
Growth on ANA blood plate and LKV plate after 48 hour anaerobic incubation. (no hemolysis)
Subsequent culture of those colonies to an aerobic chocolate plate resulted in no growth.
Blackening was observed on BBE agar.
Special potency disks:
Kanamycin-resistant
Vancomycin-resistant
Colistin-resistant
What is the most likley ID?
Bacteroides fragilis
A specimen was isolated from the cerebrospinal fluid of a 4 yr old girl visiting from outside the US. The following results were observed after 48 hr incubation in CO2.
Choc = small grey colonies with a mouse-like odor
Gram stain= gram negative coccobacilli
Blood agar= no growth
MacConkey = no growth
Satteliting with Staphylococcus = positive
ALA= neg
The most likely organism is:
Haemophilus influenzae
A sputum specimen was obtained from a 79 year old male. He has COPD (chronic obstructive pulmonary disease). The xrays suggest pneumonia.
His culture results are as follows:
Growth on Chocolate and Blood agar: large opague colonies
Macconkey= no growth
Gram stain = gram negative diplococci
satellite test = negative
Oxidase=positive
Carbohydrate fermentation: Glucose=neg, Maltose=neg, Sucrose=neg, Lactose=neg
Dnase=positive
Beta lactamase=negative
Moraxella catarrhalis
Cultivation of H. ducreyi will require all of the following except: 4-7 day incubation X Factor V Factor high humidity
V Factor
Actinomyces is usually involved in infections of
Oral/facial area
A post surgical drainage from an abdominal wound grew an anaerobic organism on a blood agar plate at 48 hours. It is not aerotolerant. The gram stain showed gram neg bacilli. The following reactions were observed:
Nitrate = neg
Catalase= negative
Growth on BBE = neg (no blackening)
Kanamycin = sensitive ,Vancomycin = resistant , Colistin= sensitive
The organism will likely be identified as:
Fusobacterium nucleatum
The ingestion of honey is not recommended for young children because it may contain the spores of this anaerobic organism.
Clostridium botulinum
