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MDL 251 Microbiology > Unit 2 > Flashcards

Unit 2 Flashcards

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1
Q

A gram positive cocci recovered from a urine culture of a 15 year old girl is alpha hemolytic, bile esculin positive and optochin negative and is most likely:

A

E. faecalis

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2
Q

The primary reservoir for S. aureus is the:

A

anterior nares

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3
Q

Which enzymes contribute to the virulence of S. aureus?

A

hyaluronidase and coagulase

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4
Q

True or False?

The normal habitat of Bacillus anthracis is the human nasopharynx.

A

False

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5
Q

Which morphologic feature is a major cinsideration in determining an identification strategy for streptococci?

A

Hemolytic pattern

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6
Q

Which group of streptococci is associated with the production of pyrogenic exotoxin (SPE) and Streptokinase?

A

Group A

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7
Q

True or False?

Initial symptoms of staphylococcal poisoning will occur 2-6 hours after eating contaminated food.

A

True

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8
Q

A black eschar on the skin is indicative of what condition.

A

Cutaneous anthrax

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9
Q

The organism that exhibits characteristic end over end tumbling motility when incubated in nutrient broth at room temperature for 1-2 hours is:

A

Listeria monocytogenes

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10
Q

The CAMP factor is produced by:

A

Streptococcus agalactiae

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11
Q

Micrococcus luteus will produce which of the following results when a modified oxidase test is performed:

A

blue/purple color

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12
Q

Patients who are immunocompromised and pregnant women should avoid eating soft cheeses such as Mexican-style, feta, brie, Camembert and blue veined cheese to prevent:

A

foodborne listeriosis

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13
Q

An organism recovered from a case of pharyngitis (sore throat) in an 8 year old child is beta hemolytic, catalase negative and has a gram positive cocci chain gram stain. Which test would be performed next to further identify this organism?

A

Lancefield grouping by latex agglutination

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14
Q

S. saprophyticus is best differentiated from S. epidermidis by resistance to:

A

Novobiocin

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15
Q 
All of these organisms are associated with gastrointestinal infections except:
Bacillus anthracis
Listeria monocytogenes
Staphylococcus aureus
Streptococcus pyogenes
A

Streptococcus pyogenes

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16
Q

A wet mount and gram stain of vaginal secretions of a patient with bacterial vaginosis caused by Gardnerella vaginalis will likely reveal:

A

Large squamous epithelial cells with numerous attached gram variable or gram negative coccobacilli and rods

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17
Q

True or False?

Sodium desoxycholate will not dissolve colonies of viridans streptococci on sheep blood agar.

A

True

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18
Q

The bacterial species that can be described as able to grow in 6.5% NaCL and 40% bile and hydrolyze esculin, is part of the normal gastrointestinal flora of animals and humans, frequently nonhemolytic, and is a major cause of nosocomial infections is:

A

E. faecalis

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19
Q

True or False?
Lancefield group testing by latex agglutination is appropriate for alpha heolytic, catalase negative, gram positive cocci.

A

False

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20
Q

This aerobic gram positive rod may cause conjuctivitis, gastroenteritis, meningitis and neonatal sepsis.

A

Listeria monocytogenes

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21
Q

Gram positive rods that are palisading and are typical of:

A

Corynebacterium diptheriae

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22
Q

This alpha hemolytic gram positive rod is frequently considered a contaminant and is normal flora of the human mouth and female genital tract. It is:

A

Lactobacillus sp.

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23
Q

The tube coagulase test:

A

detects extracellular coagulase (free coagulase)

24
Q

The first identification test performed on a clinical isolate of a gram-positive cocci that is catalase-positive would likely be the:

A

coagulase test

25
Q

The optochin test causes a zone of greater than 14mm with which organism?

A

S. pneumoniae

26
Q

A patient has several bloodstream cultures growing beta hemolytic, catalase positive, gram positive cocci. It is coagulase negative by the slide coagulase test. What is the most appropriate action to identify this organism?

A

do a tube coagulase to confirm the slide test

27
Q

This organism is not considered to be part of the normal flora of humans:

A

S. pyogenes

28
Q

An organism that has a gram stain of gram positive cocci in pairs is most likely:

A

S. pneumoniae

29
Q

What type of motility reaction, associated with Listeria, is known as:

A

Umbrella

30
Q

The gram stain of LIsteria monocytogenes may be confused with streptococci, the organisms are rapidly differentiate by this test:

A

Catalase

31
Q

What mode of transmission has not been associated with B. anthracis infection?

A

Person to person

32
Q

True or False?

Group A Streptococcus is PYR positive and CAMP postive.

A

False

33
Q

A non-spore forming, catalase negative, gram positive rod that produced hydrogen sulfide was isolated from a finger abcess of a farm worker. The organism is likely:

A

Erysipelothrix rhusiopathiae

34
Q

A 3 day old infant is symptomatic of meningitis, which organism is most likely the cause?

A

Streptococcus agalactiae

35
Q 
Which of the following infections is least likely to be associated with S. aureus?
Neonatal Sepsis
Osteomyelitis
Foodpoisoning
Toxic Shock Syndrome
A

Neonatal Sepsis

36
Q

The gram stain morphology of Staphylococcus epidermidis is typically:

A

Gram positive cocci in clusters

37
Q

Optochin, bile solubility, bile esculin, and 6.5% NaCl are tests most likely performed on:

A

alpha-hemolytic streptococcus

38
Q

The catalase test is used to differentiate which groups of organisms?

A

Staphylococcus from Streptococcus

39
Q 
A patient is admitted to the hospital with a persistent cough, fever and chills.  Sputum cultures were collected and subsequently grew and organism with the following results:
blood agar - mucoid, alpha hemolytic colonies
chocolate - growth
MacConkey - no growth
PEA - light growth
Gram stain - gram pos cocci in pairs (diplococci)
catalase - neg
bile esculin - negative
6.5% salt - negative
bile solubility - lysis of the colony
Optochin zone - 16mm
The most likely identification is:
A

Streptococcus pneumoniae

40
Q 
An oncology patient receiving chemotherapy was seen by her physician for a soft tissue infection of her lower leg.  A routine culture grew the folowing:
blood agar - beta hemolytic colonies
PEA and Thioglycollate - growth
MacConkey - no growth
Gram stain - gram positive cocci
catalase - negative
PYR - negative
Motility negative
CAMP test - positive
Bile esculin - negative
What is the most likely presumptive ID?
A

Streptococcus agalactiae

41
Q

An organism was recovered from a blood culture of a 41 year old female cattle rancher from South America. The patient was in acute respiratory distress and experiencing multiple organ failure. The lab results were:
Gram stain - large gram positive rods with spores
The plates were than handled using special precautions.
Colonies on aerobic blood agar plate are nonhemolytic with swirling progections.
PEA - growth
MacConkey - no growth
H2S negative
Motility - negative
The organism was sent to the Virginia Consolidated labs for further ID.
What is the expected ID of this organism?

A

Bacillus anthracis

42
Q 
A wound culture of a 17 year old football player is growing a pure culture of an organism with the following results:
blood agar - cream colored, beta hemolytic colonies
growth on PEA no growth on MacConkey
Gram stain - GPC in clusters
Catalase - positive
CAMP - negative
PYR Test - negative
Slide coagulase - negative
Novobiocin - sensitive
Tube coagulase - positive
A

S. aureus

43
Q 
Heavy growth of a bacteria was recovered from a urine culture of a 29 year old female.  The following results were observed:
blood agar - pure culture of a white non hemolytic colony
PEA - growth
MacConkey - no growth
Gram stain - GPC
Catalase - positive
Slide coagulase - negative
Tube coagulase - negative
Novobiocin - resistant
Modified oxidase - neg
A

Staphylococcus saprophyticus

44
Q

Match the organism with the appropriate disease or infection:
Severe conjuctivitis, corneal ulceration

A

Listeria monocytogenes

45
Q

Match the organism with the appropriate disease or infection:
Otitis media

A

Streptococcus pneumoniae

46
Q

Match the organism with the appropriate disease or infection:
Nosocomial UTI

A

Enterococcus faecalis

47
Q

Match the organism with the appropriate disease or infection:
Carbuncles

A

Staphylococcus aureus

48
Q

Match the organism with the appropriate disease or infection:
Bacterial vaginosis

A

Gardnerella vaginalis

49
Q

Match the organism with the appropriate disease or infection:
Scarlet fever

A

Streptococcus pyogenes

50
Q

Match the organism with the appropriate disease or infection:
Cellulitis in butchers

A

Erysipelothrix rhusopathiae

51
Q

Match the organism with the appropriate disease or infection:
Wool sorter’s disease

A

Bacillus anthracis

52
Q

Match the organism to the appropriate media:

PLAT

A

Bacillus anthracis

53
Q

Match the organism to the appropriate media:

Cysteine tellurite

A

Corynebacterium diphtheriae

54
Q

Match the organism to the appropriate media:

Oxford

A

Listeria monocytogenes

55
Q

Match the organism to the appropriate media:

Human blood bilayer tween

A

Garderella vaginalis

56
Q

The red line alert test is an immunochromatographic test that will presumptively identify:

A

Bacillus anthracis

57
Q

Stabbing the loop into the blood agar after streaking could allow colonies to display hemolysis caused by:

A

Streptolysin O

MDL 251 Microbiology (4 decks)

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