Unit 4

Question 1

Give the 5 stages of micropropagation

Answer 1

Stage 0 - Selection of mother plant for explants isolation
Stage1 - Explant establishment in culture medium
Stage2 - Proliferation and multiplication
Stage 3 - Plant establishment and rooting
Stage 4 - Acclimatization or Hardening

Question 2

Give the Culture and Medium Culture Techniques

Answer 2

1. Meristem culture
2. Callous Culture
3. Cell Culture
4. Embryo culture
5. Anther culture
6. Somatic Embryogenesis
7. Protoplast Culture

Question 3

Modified and specialized stem structure in which the main axis of the plant grows
horizontally at or just below the soil surface

Answer 3

Rhizomes

Question 4

Refers to the production of plants from very small plant parts, tissues or cells, grown
aseptically in a test tube or containers under controlled nutritional, environmental and aseptic conditions.

Answer 4

Micropropagation

Question 5

A complicated structure, which consists of a short-thickened stem bearing roots on the underside and thick fleshy leaves on the upper side.

Answer 5

Bulbs

Question 6

A swollen, modified underground stem, which primarily function as the storage organ of the plant.

Answer 6

Tuber

Question 7

A specified long slender stem that develops from the axil of leaf at the crown of a plant, which prostate horizontally along the ground.

Answer 7

Runners

Question 8

A shoot, which arises on a plant below the ground, arising from an adventitious bud on a root.

Answer 8

Suckers

Question 9

A short, solid, much swollen underground stem, enclosed by dry scale like leaves, with one or several buds near the top

Answer 9

Corm

Question 10

an enlarged fleshy stem with several nodes produced by some horticultural plants (mostly orchids), mainly for the storage of food.

Answer 10

Pseudobulbs