Unit 3.5 - Energy transfers in and between organisms Flashcards
what is photosynthesis?
its the process where energy from light is used to make glucose from H20 and CO2. the light energy is converted to chemical energy in the form of glucose
6H2O + 6CO2 -> Energy -> C6H12O6 + 6O2
where is energy stored in plants?
- its stored in the glucose until the plants release it by respiration. animals obtain glucose by eating plants or other animals then respire the glucose to release energy
how do plants and animals release energy?
- they release energy from glucose in respiration, which is used in all biological processes.
- aerobic respiration uses oxygen and anaerobic respiration is without oxygen
what does anaerobic respiration make?
in plants and yeast it produces ethanol and carbon dioxide and releases energy. in humans, it produces lactase and releases energy
what happens to the energy in respiration?
the energy released from glucose is used to make ATP which carries energy around the cell to where its needed. ATP is synthesised via a condensation reaction between ADP and inorganic phosphate using energy from and energy-releasing reaction.
describe and energy-releasing reaction?
eg the breakdown of glucose in respiration. the energy is stored as chemical energy in the phosphate bond. the enzyme ATP synthase catalyses the reaction.
what happens after ATP synthase catalyses the reaction?
- ATP diffuses to the part of the cell that needs energy. here its hydrolysed back into ADP and inorganic phosphate. chemical energy is released from the phosphate bond and used by the cell. ATP hydrolase catalyses this reaction.
- the ADP and Pi are recycled and it starts again
what are the pros of ATP?
- it releases energy in a small, manageable amounts so none is wasted as heat
- its soluble so can be transported easily
- easily broken down so can be released instantaneously
- make other molecules more reactive by transferring a phosphate to them (phosphorylation)
- it cant pass out the cell so the cell always has an immediate supply of energy
what is a metabolic pathway?
a series of small reactions controlled by enzymes eg photosynthesis
what is phosphorylation?
adding phosphate to a molecule
what is photophosphorylation?
adding phosphate to a molecule using light
what is photolysis?
the splitting (lysis) of a molecule using light (photo) energy
what is photoionisation?
when light energy ‘excites’ electrons in an atom or molecule, giving them more energy and causing them to be released. the release of electrons causes the atom or molecule to become a positively charged ion
what is hydrolysis?
the splitting (lysis) of a molecule using water (hydro)
what is decarboxylation?
the removal of co2 from a molecule
what is dehydrogenation?
the removal of hydrogen from a molecule
what are redox reactions?
reactions that involve oxidation and reduction
what is a coenzyme?
a molecule that aids the function of an enzyme. they work by transferring a chemical group from one molecule to another
what is the coenzyme in photosynthesis?
its NADP which transfers hydrogen from one molecule to another which means it can reduce (give hydrogen) or oxidise (take hydrogen from) a molecule
what do chloroplasts contain?
they have photosynthetic pigments like chlorophyll a, chlorophyll b and carotene. these are coloured substances that absorb light energy needed for PHS.
- they are found in the thylakoid membranes where they’re attached to proteins.
what is a photosystem?
its made up of a protein and pigment together. there are two photosystems used by plants to capture light energy.
- photosystem I (PSI) and photosystem II (PSII)
what is stroma?
- its contained within the inner membrane of the chloroplast and surrounds the thylakoid. its a gel like substance and contains enzymes, sugars and organic acids
what is the energy resulting from the photoionisation of chlorophyll used for?
1 - making ATP from ADP and Pi in photophosphorylation
2 - making reduced NADP from NADP
3 - splitting water into protons (H+ ions), electrons and oxygen in photolysis
what are photosystems linked by?
- they are linked by electron carriers, which are proteins that transfer electrons. the photosystems and electron carriers form an electron transport chain- a chain of proteins which excited electrons flow through
what is Step 1 of the LDR?
light energy is absorbed by PSII which excites electrons in the chlorophyll. the electrons move to a higher energy level to where they’re released from the chlorophyll and move down the electron transport chain to PSI.
the chlorophyll has been photoionised
what is Step 2 of the LDR?
as the excited electrons leave PSII to move down the transport chain, they must be replaced. light energy splits water into protons (H+ ions), electrons and oxygen in photolysis.
(so the O2 in PHS comes from water and is made in the LDR = H20 -> 2H+ + 0.5O2
what is Step 3 of the LDR?
the electrons lose energy as they move down the chain. this energy is used to transport protons into the thylakoid, so that it has a higher concentration of protons than the stroma.
- this forms a proton gradient across the membrane, where they move down into the stroma via ATP synthase which is embedded in the thylakoid membrane. the energy from their movement combines ADP and Pi to ATP.
what is Step 4 of the LDR?
light energy is absorbed by PSI, which excites the electrons to a higher energy level. the electrons are transferred to NADP, along with a proton (H+ ion) from the stroma to form reduced NADP.
what is chemiosmosis?
its the process of electrons flowing down the electron transport chain and creating a proton gradient across the membrane to drive ATP synthesis
what is cyclic photophosphorylation?
it only uses PSI. it is cyclic as the electrons from the chlorophyll molecule aren’t passed onto NADP, but they’re passed back to PSI via the electron carriers. this means the electrons are recycled and can flow through PSI.
- this process produces small amounts of ATP only
what is the Light independent reaction/calvin cycle?
it takes place in the stroma of the chloroplasts. it makes a molecule called triose phosphate from CO2 and ribulose bisphosphate
what is Step 1 of the calvin cycle?
CO2 enters the leaf through the stomata and diffuses into the stroma of the chloroplast. it’s combined with ribulose bisphosphate RuBP, a 5cc. reaction is catalysed by enzyme rubisco.
this makes in unstable 6-carbon compound, which breaks down into 2 molecules of 3-carbon compound called glycerate 3-phosphate, GP
what is Step 2 of the calvin cycle?
the hydrolysis of ATP from LDR provides energy to turn the GP into a different 3-carbin compound called triose phosphate TP. this reaction require H+ ions which come from the reduced NADP from the LDR.
some TP is converted into useful organic compounds eg glucose and some continues in the cycle to regenerate RuBP
what is Step 3 of the calvin cycle?
five out of every six molecules of TP produced in the cycle aren’t to make hexose sugars, but to regenerate RuBP. regenerating RuBP uses the rest of the ATP produced by the LIR.
how are carbohydrates made?
hexose sugars eg glucose are made by joining two triose phosphate molecules together and larger carbohydrates are made by joining hexose sugars together in different ways
how are lipids and amino acids made?
lipids - made using glycerol which is synthesised from triose phosphate and fatty acids which are synthesised from glycerate 3-phosphate
amino acids - some are also made from glycerate 3-phosphate
how does light intensity affect PHS?
light is needed to provide the energy for the LDR so the higher the intensity of light, the more energy it provides. only certain wavelengths of light are used eg chlorophyll a and b and carotene only absorb red and blue light in sunlight
how does temperature affect PHS?
PHS involves enzymes therefore if it falls below optimum enzymes become inactive but if its too high they may start to denature. also at high temps, stomata close to avoid losing too much water which causes PHS to slow down as less CO2 enters
how does CO2 affect PHS?
having too high CO2 levels means the stomata start to close
- also plants need a constant supply of water, too little PHS has to stop but too much and the soil becomes waterlogged reducing uptake of minerals
describe glycolysis? A
it involves splitting one molecule of glucose (with 6C) into two smaller molecules of pyruvate (3C). the process happens in the cytoplasm of cells outside the mitochondria, its an anaerobic process.
what happens in phosphorylation in glycolysis? A
glucose is phosphorylated using a phosphate from a molecule of ATP. this creates 1 molecule of glucose phosphate and 1 molecule of ADP. ATP is then used to add another phosphate, forming hexose bisphosphate, which is then split into 2 molecules of TP
what happens in oxidation in glycolysis? A
TP is oxidised forming 2 molecules of pyruvate. NAD collects the hydrogen ions, forming 2-reduced NAD. 4 ATP are produced, but 2 were used up in phosphorylation, so there’s a net gain of 2 ATP
describe the link reaction? B
pyruvate is decarboxylated (one carbon atom is removed from pyruvate in the form of CO2). pyruvate is oxidised to form acectate and NAD is reduced to form NAD, the acectate is combined with coenzyme A (CoA) to form acetyl coenzyme A (acetyl CoA). no ATP is made.
how many pyruvate molecules are made for every glucose?
two pyruvate molecules are made for every glucose molecules that enters glycolysis. this means the link reaction and the krebs cycle happen twice for every glucose molecule.
describe the molecules that go into krebs cycle?
1- two molecules of acetyl CoA go into the krebs cycle
2- two CO2 molecules are released as a waste product of respiration
3- two molecules of reduced NAD are formed and go into the last stage (oxidative phosphorylation)