Unit 3 (pre-test 1)

Question 1

what are the 4 methods of transport

Question 2

Explain the Ran-GTP gradient and how it drives nuclear import/export + how it is reestablished

Question 3

what regulates NPC transport?

Question 4

What are the 3 types of proteins that bind import receptors during their normal function while they import proteins into the nucleus

Question 5

Why can small molecules freely diffuse in/out of the nucleus while larger ones can’t

Question 6

whats the max size for free nucleus diffusion

Question 7

explain subcellular fractionating and how we do it

Question 8

how do we prove a signal sequence is necessary and sufficient for localization of a protein to a specific compartment

Question 9

explain what a sorting signal is and what happens to proteins that don’t have one

Question 10

explain the difference between a sorting sequence and a sorting patch

Question 11

is the plasma membrane a small or large fraction of the total membrane in a eukaryotic cell?

Question 12

how is nuclear import/export regulated

Answer 12

answer relates to the avalibility of the NLS sequence

Question 13

how does chloroplast transport compare to mitochondrial transport

Question 14

what are Ran’s other jobs

Question 15

what do GEFs and GAPs do? What do they stand for?

Question 16

How do nuclear import receptors get into the nucleus? what do they bind to?

Question 17

explain the process of nuclear import

Question 18

how is the GTP/GDP gradient maintained during mitosis?

Question 19

what is NPC?

Question 20

what does the nuclear membrane look like? what organelle is it continous with?

Question 21

what is the nuclear import sequence and where would you find it on a polypeptide?

Question 22

What is rich in K and R (relating to nuclear transport)

Question 23

what moves in and out of the nucleus?

Question 24

what are the 4 evolutionary cell compartment classes

Question 25

Explain what the following organelles are/ do:
-ER
-Golgi
-Mitochondria
-Chloroplasts and other plastids
-lysosomes

Question 26

are peroxisomes essential to life?

Question 27

what are the 2 types of peroxisomes that plants have?

Question 28

Where do peroxisomes come from?

Question 29

What is the peroxisome import sequence? where is it?

Question 30

What do peroxisomes do?

Question 31

How do things get into the peroxisome

Question 32

what are peroxins

Question 33

how do proteins get into the IMM

Question 34

how do proteins get into the Mitochondrial matrix

Question 35

how do proteins get into the IMS

Question 36

how do proteins get into the OMM

Question 37

What signal is common for mitochondrial transport

Question 38

what is HSP 70

Question 39

why do so many metabolites end up in the IMS

Question 40

what is required for mitochondrial transport?