Unit 2 Flashcards
How are phospholipids distributed in the outer and inner leaflet? How is chlolesterol?
PS (serine) and PE (ethanolamine) are in the inner leaflet.
PC (choline) and SM (sphingomyelin) are mostly in the outer leaflet
cholesterol is around equal in both.
where would I find glycolipids?
Only on the outside and also only of the apical side of epithelial cells.
What does Glycocalyx do?
It protects the cell from damage (mechanical and chemical)
May aid in cell recognition
Prevents unwanted protein interactions that would decrease fluidity
what are the two main secondary structures for membrane proteins
what secondary structure do transmembrane proteins typically have?
what secondary structrue do monolayer proteins always have?
why is it sometimes easier to find the structure of b-barrels
are all pore b-barrels? are all b-barrel pores?
what are the 4 main types of phospholipids?
which phospholipid is negative?
What is the difference between isolating integral proteins and periferal proteins?
what are some methods to isolate peripheral proteins?
whats the most common method for isolating integral proteins?
what are detergents?
what are the 2 types of detergents?
what is the critical micellar concentration
how do detergents work?
what are nanodiscs?
what are the 2 factors that determine fluidity?
how does tail size affect fluidity?
how does cholesterol affect fluidity?
what is the main function of cholesterol?
how does saturation affect membrane fluidity?
how does temperature affect fluidity? And, what is a phase transition?
What tehcnique would we use to show that proteins move laterally in the membrane?
What are 5 reasons why FRAP might show that proteins are not moving laterally?
what forces hold the bilayer together?
what is the purpose of a hydropathy plot? how do they work and what proteins do they work for?
what are the 3 main types of lipid movement? what is the secrete forth?
which forms of lipid movement are “fast” , which is fastest and which is really rare?
What is a transmembrane protein? what are the 3 types of transmembrane proteins?
what do transmembrane proteins usually do? (4 things)
what are the ways in which a monolayer protein interacts with the lipid bilayer?
What are the 3 types of membrane proteins?
What is the definition of ampiphllic
Explain what lipid rafts are
Explain what lipid droplets are and how they are formed.
Explain how keeping PS on the inside leaflet helps with cell signaling
Where does the glycacolyx come from?
what would we use Ruthemium red stain for?
what is the difference between glycoprotein and proteoglycan? where do they come from and how do they associate with the membrane? What other sugar molecule associates in the same way?
