UNIT 3 - Laboratory Identification Of Fungi

Question 1

Respiratory Tract Secretions:

Answer 1

○ Sputum
○ Induced sputum
○ Bronchial washings
○ Bronchoalveolar lavage
○ Tracheal aspirations

Question 2

Antibacterial antibiotics is included in the battery of media to:

Answer 2

○ Unsure optimal recovery of fungi
○ Prevent overgrowth by contaminants such as
bacteria from the normal flora
○ To prevent the growth of molds

Question 3

CSF is a Specimen used to detect the presence of

Answer 3

Cryptococcus neoformans

Question 4

media used for ascopore development

Answer 4

Ascopore medium, V-8 agar

Question 5

Medium for demonstration of melanin in Cryptococcus neoformans

Answer 5

Birdseed (niger) agar

Question 6

medium used to enhanced recovery of dimorphic fungi

Answer 6

Brain-heart infusion agar with blood

Question 7

medium used for differentiation of trichophyton rubrum and T. mentagrophytes

Answer 7

Christensen urea agar

Question 8

medium used for chromogenic mixture to identify yeast species

Answer 8

CHROMagar Candida

Question 9

medium used for visualization of yeast morphology

Answer 9

Cornmeal agar with Tween 80

Question 10

medium used for identification of Aspergillus species

Answer 10

Czapek-Dox Agar

Question 11

medium used for pigment development of Trichophyton rubrum and mould morphology

Answer 11

Potato dextrose agar

Question 12

General purpose fungal growth medium (traditional formulation or Emmon’s modification)

Answer 12

Sabouraud dextrose (SAB) agar

Question 13

Selective medium to inhibit saprophytic fungi (antibacterials such as chloramphenicol or gentamicin also included)

Answer 13

SAB agar with cycloheximide

Question 14

medium used for study of microscopic structure

Answer 14

Slide culture (various agars)

Question 15

medium used for differentiation of Trichophyton species

Answer 15

Trichophyton agars

Question 16

Temporary Mounts

Answer 16

• 10-20% KOH
• Lactophenol cotton blue
• India ink or Nigrosin
• Calcofluor white stain