UNIT 3 Flashcards
Polymer
a molecule made up of a large
number of smaller, repeating units
Monomer
a molecule that forms bonds with
other identical molecules as the
repeating units that make up a polymer
Nucleotide
the monomer (building block) of
nucleic acids which are joined together
to form DNA or RNA (polymers);
consists of a phosphate group, sugar and
nitrogenous base
Complementary
the term used to describe
the fact that a nitrogenous base
can only pair with one other
nitrogenous base (cytosine is
complementary to guanine,
adenine is complementary
to thymine)
Gene expression
conversion of the code in DNA
of a gene into a protein through
protein synthesis
Transcription
the process through which DNA is converted to messenger RNA
(mRNA) and the genetic code
in the DNA is copied to the
mRNA
Enzyme
a type of protein also referred to
as a biological catalyst, that
speeds up reactions within
an organism by lowering
activation energy
Promoter
the region of a gene at which
RNA polymerase binds, to initiate
transcription
Terminator
the region of a gene at which transcription
stops and the RNA polymerase dissociates from the strand
Intron
a region of a gene that contains
sequences that do not code for
the protein to be expressed
Exon
a region of a gene that
contains genetic information that codes for the
specific protein to be synthesised
Translation
the process through which the information
in mRNA is converted into a sequence of
amino acids to synthesise a
protein
Codon
bases in mRNA that code for a specific amino
acid
Anticodon
a set of three bases on tRNA that are complementary to codons in mRNA
Amino acid
the monomer that forms polypeptide chains and proteins
Universal triplet code
the genetic coding system
based on codons with three bases,
shared by most organisms
Degenerate
describes a genetic code in which multiple codons code for the same amino acid; also
referred to as redundant
Mutation
a permanent change in the
nucleotide sequence of a section of DNA
Biomacromolecule
a large biological polymer, such as a protein, a nucleic acid or a carbohydrate
Peptide bond
a chemical bond between two
amino acids
Polypeptide
a long chain of amino acids
forming part of a protein
Condensation reaction
a reaction in which two molecules are joined to make a larger molecule, resulting in the loss of a smaller molecule as another product (in organisms, this is usually
water)
Proteome
the complete collection of proteins within
an organism at a given time
Coding region
the introns and exons of a gene that is
transcribed into pre-mRNA
Operator
a section of DNA code where the repressor
protein can bind
Repressor
a regulatory protein that binds to DNA,
inhibiting transcription
Regulatory gene
a region of DNA that codes for
a regulatory protein, which controls the
expression of other genes
Structural gene
a region of DNA that codes for a protein that performs a specific function for a cell or organism
Operon
a series of genes under the control of a single promoter and operator
Restriction enzyme
a bacterially produced protein that cuts DNA at a specific sequence of nucleotides
called a recognition site; also known as a restriction endonuclease
Recognition (restriction) site
a specific sequence of nucleotides that
is the location for a restriction
enzyme to cut
Digestion
(in the context of restriction enzymes) a
reaction using an enzyme to break down large molecules
Sticky ends
short lengths of unpaired
nucleotides in DNA resulting from
a staggered cut by a restriction
enzyme
Blunt ends
short lengths of fully paired
nucleotides in DNA resulting from
a straight cut by a restriction
enzyme
Palindrome
a sequence that reads the same in both
directions
DNA ligase
an enzyme that joins two pieces
of DNA at their sugar–phosphate
backbone
Primer
synthetic singlestranded piece of DNA (or RNA) complementary to a specific sequence of nucleotides
Gel electrophoresis
a technique used to separate different-sized fragments of DNA (or protein)
DNA standard
a DNA sample that contains fragments of
DNA of known size that is used to compare the sizes of unknown DNA fragments in base pairs or kilo base pairs; also known as a DNA ladder
DNA profiling
a method of DNA analysis in which
regions of DNA from different individuals are analysed and compared
Variable number tandem repeats (VNTRs)
a region of a chromosome that shows variation between individuals in length and
number of repeats of nucleotide sequences; also referred to as short tandem repeats (STRs) when 2–6 base
pairs long
Allele
an alternative form of a gene
Homologous chromosomes
chromosomes that have
matching structural
features (size, banding pattern,
centromere location) and
gene loci
Genetic screening
DNA profiling to determine
whether an individual
is carrying a particular gene
for a disorder