UNIT 2.2 CULTURE MEDIA Flashcards
SELECTIVE
For the isolation of Mycoplasma and Ureaplasma spp.
E AGAR
BIOCHEMICAL CHARACTERISTIC MEDIUM
For the identification of organisms which utilize acetamide as a source of carbon such as Pseudomonas aeruginosa
Acetamide slant
DIFFERENTIAL MEDIUM
Used to distinguish Escherchia coli from Shigella spp.
acetate agar
BIOCHEMICAL CHACTERISTIC MEDIUM
For the identification of organisms which utilize acetate as a source of carbon such as Escherichia coli
ACETATE SLANT
ENRICHMENT
Increases the number of pathogenic Vibrio in the specimen
ALKALINE PEPTONE WATER
SELECTIVE
For the isolation of
Mycobacterium spp.
American Thoracic Society medium ATS
SELECTIVE
For isolation of mycobacteria, particularly Mycobacterium tuberculosis from clinical specimens.
American Trudeau Society Medium
TRANSPORT
Maintains the viability of the organisms from source to the laboratory
ANIE’S MEDIUM
REDUCING OR ANAEROBIC
For isolation of all anaerobes
ANAEROBIC BLOOD AGAR
REDUCING OR ANAEROBIC
For isolation of
gram-negative anaerobes, especially Bacteroides, Prevotella, and some Fusobacterium
Anaerobic Kanamycin Vancomycin Blood Agar
REDUCING OR AEROBIC
and some Fusobacterium. Detection of pigment production
of Prevotella
anaerobic PAROMOYCINE VANCOMYCIN
REDUCING OR ANAEROBIC
For isolation of obligate gram-positive and gram-negative anaerobes. It inhibits growth of enteric bacilli and swarming of Proteus.
ANAEROBIC PHENYLETHYL ALCOHOL AGAR
SELECTIVE AND DIFFERENTIAL
Selective for Bacteroides spp. Used to differentiate bile-resistant from bile-sensitive Bacteroides spp.
BBE BACTEROIDES BILE ESCULINE
Selective for group D
Streptococcus and Enterococcus
BEM BILE ESCULIN MEDIUM
SELECTIVE
For the isolation
Brucella spp.
Biphasic media of Brucella Broth and Agar in Castañeda bottle or Septi- check
SELECTIVE
Selective for the isolation of Salmonella typhi
Bismuth Sulfite Agar BSA or Wilson Blair Agar
ENRCED AND DIFFERENTIAL
Enriched with unheated blood; differentiates organism through hemolytic pattern
BLOOD AGAR
SELECTIVE
For the isolation
Francisella tuarensis
Blood Cysteine Glucose Agar with thiamine
Selective
Selective for the isolation of Bordetella pertussis
BORDET GENGOU AGAR
BASIC OR GENERAL PURPOSE MEDIUM
Basic medium for the isolation of microorganisms and can be used for preparing ure culture for antibiotic susceptibility test
BRAIN HEART INFUSION
For the isolation of
Brucella spp.
BRUCELLA BLOOD AGAR
SELECTIVE
For the isolation of
Legionella pneumophila
BCYE
SELECTIVE
For isolation of Legionella spp. From clinical specimens. It can also be used to isolate Francisella and Nocardia spp.
BCYE WITH CYSTEINE (pag to isolate francisella may cysteine)
For isolation of
Burkholderia cepacia
from respiratory specimens
SELECTIVE
BURHOLDERIA CEPACIA AGAR
SELECTIVE
For isolation and cultivation of Campylobacter spp. From stoll specimens
CAMPYLOBACTER BLOOD AGAR
Less inhibitory for
Campylobacter spp.
Than other selective media but more inhibitory for organisms found as normal fecal microbiota
CAMPYLOBACTER CHARCOAL AGAR
SELECTIVE AND TRANSPORT
CAMPYLOBACTER SPP
Campylobacter thioglycollate broth Campy-thio)
TRANSPORT
MAintains the viability of the organism to the source of the laboratory
CARY BLAIRE MEDIUM
For the isolation of
Campylobacter spp.
CEFOPERAZONE VANCOMYCIN AMPHOTERICIN MEDIUM
Yersinia enterocolitica
in stool samples
CEFSULODIN IRGASAN NOVOBIOCIN AGAR
Selective for
Pseudomonas aeruginosa and inhibitory for other Pseudomonas spp. Except for P. fluorescens.
CETRIMIDE AGAR
Isolates and identifies
pseudomonas aeruginosa
CETRIMIDE AGAR SLANT
