UNIT 2.2 CULTURE MEDIA

Question 1

SELECTIVE

For the isolation of Mycoplasma and Ureaplasma spp.

Answer 1

E AGAR

Question 2

BIOCHEMICAL CHARACTERISTIC MEDIUM

For the identification of organisms which utilize acetamide as a source of carbon such as Pseudomonas aeruginosa

Answer 2

Acetamide slant

Question 3

DIFFERENTIAL MEDIUM

Used to distinguish Escherchia coli from Shigella spp.

Answer 3

acetate agar

Question 4

BIOCHEMICAL CHACTERISTIC MEDIUM

For the identification of organisms which utilize acetate as a source of carbon such as Escherichia coli

Answer 4

ACETATE SLANT

Question 5

ENRICHMENT

Increases the number of pathogenic Vibrio in the specimen

Answer 5

ALKALINE PEPTONE WATER

Question 6

SELECTIVE

For the isolation of
Mycobacterium spp.

Answer 6

American Thoracic Society medium ATS

Question 7

SELECTIVE

For isolation of mycobacteria, particularly Mycobacterium tuberculosis from clinical specimens.

Answer 7

American Trudeau Society Medium

Question 8

TRANSPORT

Maintains the viability of the organisms from source to the laboratory

Answer 8

ANIE’S MEDIUM

Question 9

REDUCING OR ANAEROBIC

For isolation of all anaerobes

Answer 9

ANAEROBIC BLOOD AGAR

Question 10

REDUCING OR ANAEROBIC
For isolation of
gram-negative anaerobes, especially Bacteroides, Prevotella, and some Fusobacterium

Answer 10

Anaerobic Kanamycin Vancomycin Blood Agar

Question 11

REDUCING OR AEROBIC

and some Fusobacterium. Detection of pigment production
of Prevotella

Answer 11

anaerobic PAROMOYCINE VANCOMYCIN

Question 12

REDUCING OR ANAEROBIC

For isolation of obligate gram-positive and gram-negative anaerobes. It inhibits growth of enteric bacilli and swarming of Proteus.

Answer 12

ANAEROBIC PHENYLETHYL ALCOHOL AGAR

Question 13

SELECTIVE AND DIFFERENTIAL
Selective for Bacteroides spp. Used to differentiate bile-resistant from bile-sensitive Bacteroides spp.

Answer 13

BBE BACTEROIDES BILE ESCULINE

Question 14

Selective for group D
Streptococcus and Enterococcus

Answer 14

BEM BILE ESCULIN MEDIUM

Question 15

SELECTIVE
For the isolation
Brucella spp.

Answer 15

Biphasic media of Brucella Broth and Agar in Castañeda bottle or Septi- check

Question 16

SELECTIVE

Selective for the isolation of Salmonella typhi

Answer 16

Bismuth Sulfite Agar BSA or Wilson Blair Agar

Question 17

ENRCED AND DIFFERENTIAL
Enriched with unheated blood; differentiates organism through hemolytic pattern

Answer 17

BLOOD AGAR

Question 18

SELECTIVE
For the isolation
Francisella tuarensis

Answer 18

Blood Cysteine Glucose Agar with thiamine

Selective

Question 19

Selective for the isolation of Bordetella pertussis

Answer 19

BORDET GENGOU AGAR

Question 20

BASIC OR GENERAL PURPOSE MEDIUM

Basic medium for the isolation of microorganisms and can be used for preparing ure culture for antibiotic susceptibility test

Answer 20

BRAIN HEART INFUSION

Question 21

For the isolation of
Brucella spp.

Answer 21

BRUCELLA BLOOD AGAR

Question 22

SELECTIVE
For the isolation of
Legionella pneumophila

Answer 22

BCYE

Question 23

SELECTIVE

For isolation of Legionella spp. From clinical specimens. It can also be used to isolate Francisella and Nocardia spp.

Answer 23

BCYE WITH CYSTEINE (pag to isolate francisella may cysteine)

Question 24

For isolation of
Burkholderia cepacia
from respiratory specimens

Answer 24

SELECTIVE
BURHOLDERIA CEPACIA AGAR

Question 25

SELECTIVE

For isolation and cultivation of Campylobacter spp. From stoll specimens

Answer 25

CAMPYLOBACTER BLOOD AGAR

Question 26

Less inhibitory for
Campylobacter spp.
Than other selective media but more inhibitory for organisms found as normal fecal microbiota

Answer 26

CAMPYLOBACTER CHARCOAL AGAR

Question 27

SELECTIVE AND TRANSPORT

CAMPYLOBACTER SPP

Answer 27

Campylobacter thioglycollate broth Campy-thio)

Question 28

TRANSPORT
MAintains the viability of the organism to the source of the laboratory

Answer 28

CARY BLAIRE MEDIUM

Question 29

For the isolation of
Campylobacter spp.

Answer 29

CEFOPERAZONE VANCOMYCIN AMPHOTERICIN MEDIUM

Question 30

Yersinia enterocolitica
in stool samples

Answer 30

CEFSULODIN IRGASAN NOVOBIOCIN AGAR

Question 31

Selective for
Pseudomonas aeruginosa and inhibitory for other Pseudomonas spp. Except for P. fluorescens.

Answer 31

CETRIMIDE AGAR

Question 32

Isolates and identifies
pseudomonas aeruginosa

Answer 32

CETRIMIDE AGAR SLANT