LIST OF CUTURE MEDIA Flashcards
Selective and
Differential
For the isolation of
Escherichia coli
MacConkey Sorbitol
Agar
Selective
For the identification of
Enterobacteriaceae
species, particularly
Salmonella
Malonate Broth
Selective and
Differential
For isolation and
differentiation of mannitol
and non-mannitol
fermenting Staphylococci
Mannitol Salt agar
(MSA)
Selective
For the isolation of
pathogenic Neisseria spp.
such as Neisseria
gonorrhoeae
Martin Lewis agar
selective
For the isolation of Listeria
monocytogenes
mcbride medium
Tissue cell culture
For the isolation of
Chlamydia
mccoy cells
mcleod’s agar
For the identification of
Corynebacterium
diphtheriae
selective
For the identification of
gram-negative enteric
bacilli based on their
ability to ferment glucose
via mixed acid
fermentation or butylene
glycol pathway
Methyl Red
Voges-Proskauer
Test(MRVP) or
Peptone Glucose
Broth
Useful in distinguishing
among members of the
family Enterobacteriaceae
Biochemical
Characterization
Medium
Methyl Red
Voges-Proskauer
Medium
For the isolation of
Mycobacterium species
Selective
Middlebrook 7H9
(liq) / 7H10 / 7H11
(solid)
For the isolation of
Mycobacterium species
selective
Mitchison agar
For the isolation of Borrelia
spp.
selective
Modified Kelly’s
Medium or
Barbour-Stoenner-K
elly(BSK-II)
For the recovery of
Neisseria gonorrhoeae
and Neisseria meningitidis
in a mixed microbiota
selective
Modified
Thayer-Martin Agar
For the determination of a
motile from a non-motile
organism
Selective
Motility Test
Medium
For the identification of
gram-negative enteric
bacilli based on the ability
to produce LDC (lysine
decarboxylase), ODC
(ornithine decarboxylase),
or ADH (arginine
dihydrolase)
Moeller’s broth
For antibiotic susceptibility
test of most bacteria
selective
mueller-hinton agar
For the isolation of
pathogenic spp. of
Neisseria such as
Neisseria gonorrhoeae
and Mycoplasma hominis,
and Ureaplasma
urealyticum
Selective
NYC
Used to determine
whether an organism can
reduce nitrate to nitrite or
to glucose end products
such as nitrogen
Nitrate Reduction
Broth
For the determination of a
non-fastidious organism
from a fastidious organism
basal medium
nutrient agar
For the differentiation of
organisms based on their
ability to oxidize or
ferment carbohydrate
Oxidation-Fermenta
tion medium(O-F)
For the identification of
Corynebacterium
diphtheriae
selective
Pai’s agar
Useful for culturing
anaerobes
Enriched
Peptone-Y east
Extract-Glucose
Broth(PYG)
For the isolation of
Mycobacterium spp.
selective
Petragnani medium
For the identification of
gram-negative enteric
bacilli based on their
ability to produce enzyme
deaminase
Phenylalanine
Deaminase agar
(PDA)
For the selective isolation
of gram-positive cocci and
anaerobic gram-negative
bacilli
Selective
Phenylethanol agar
(PEA)
For the isolation of
Mycoplasma spp.
Pleuropneumonia-li
ke Organism Agar
(PPLO)
For isolation of
Corynebacterium
diphtheriae
Selective,
Differential,
Enrichment
Potassium Tellurite
Blood Agar
Differentiates
beta-hemolytic
streptococci and
enterococci based on their
ability to produce enzyme,
pyrrolidonyl arylamidase,
or pyroglutamyl
aminopeptidase
Biochemical
Characteristic
Medium
PYR agar
For the isolation of
Bordetella pertussis
Regan Lowe agar
For the isolation and
differentiation of
Salmonella and Shigella
Salmonella-Shigella
agar(SSA)
For the identification of
organisms based on their
ability to grow or not grow
in the presence of 6.5%
NaCl
differential medium
Salt broth(NaCl)
For the recovery of
aerobes and anaerobes
Schaedler agar
For blood culture
biphasic medium
Scott’s modified
Castaneda and
Thioglycolate broth
For enrichment and
isolation of Salmonella
spp.
enrichment
selenite broth
For differentiating
non-fermentative bacilli
such as Pseudomonas,
Acinetobacter, Alkaligenes
differential
Seller’s agar
For isolation of
Mycoplasma spp.
Selective
Shepard’s A7-B
agar
For the identification of
gram-negative enteric
bacilli based on their ability to utilize citrate as a
source of carbon
Simmons Citrate
agar(SCA)
For the isolation of
Campylobacter spp.
Selective
skirrows medium
Useful in the differentiation
of streptococci;
distinguishes
Enterococcus spp. from
group D streptococci
Sodium Chloride
Broth, 6.5%
Primary isolation medium
for Mycoplasma spp.
SP-4 Broth/Agar
For the isolation of
Streptococcus pyogenes
and Streptococcus
agalactiae.
Streptococcal
Selective Agar
(SSA)
For the isolation of
Salmonella and Shigella
spp. from stool samples.
enrichment
Tetrathionate Broth
For isolation and
differentiation of Vibrio
spp.
Thiosulfate Citrate
Bile Salts Sucrose
Agar(TCBS)
For the isolation of
Corynebacterium
diphtheriae.
tinsdale agar
For enrichment and
isolation of Streptococcus
agalactiae.
enrichment and selective
Todd-Hewitt Broth
Supplemented with
Antibiotics
For Neisseria
gonorrhoeae.
transgrow
Supports the growth of
many nonfastidious and
fastidious bacteria.
Trypticase soy agar can be
used as a base in
preparing blood agar.
general purpose medium
trypticase soy broth agar
For the identification of
gram-negative enteric
bacilli based on their
ability to produce indole.
Biochemical
Characteristic
Medium
tryptone broth
Used for the Indole Test in
the identification of
Enterobacteriaceae
species and
non-glucose-fermenting,
gram-negative bacilli.
Tryptophan Broth,
1%
Useful in identifying
species of
Enterobacteriaceae.
Biochemical
Characteristic
Medium
urea agar and broth
Useful in the isolation of
Gardnerella vaginalis.
selective and enrichment
Vaginalis Agar
For the isolation of
nontuberculous
mycobacteria such as
Mycobacterium avium.
selective
wallenstein medium
For the identification of
gram-negative enteric
bacilli and for the
differentiation of
Salmonella and Shigella
spp.
Selective and
Differential
Xylose Lysine
Deoxycholate Agar
(XLD)
