LIST OF CUTURE MEDIA

Question 1

Selective and
Differential

For the isolation of
Escherichia coli

Answer 1

MacConkey Sorbitol
Agar

Question 2

Selective

For the identification of
Enterobacteriaceae
species, particularly
Salmonella

Answer 2

Malonate Broth

Question 3

Selective and
Differential

For isolation and
differentiation of mannitol
and non-mannitol
fermenting Staphylococci

Answer 3

Mannitol Salt agar
(MSA)

Question 4

Selective

For the isolation of
pathogenic Neisseria spp.
such as Neisseria
gonorrhoeae

Answer 4

Martin Lewis agar

Question 5

selective

For the isolation of Listeria
monocytogenes

Answer 5

mcbride medium

Question 6

Tissue cell culture

For the isolation of
Chlamydia

Answer 6

mccoy cells

Question 7

mcleod’s agar

Answer 7

For the identification of
Corynebacterium
diphtheriae

selective

Question 8

For the identification of
gram-negative enteric
bacilli based on their
ability to ferment glucose
via mixed acid
fermentation or butylene
glycol pathway

Answer 8

Methyl Red
Voges-Proskauer
Test(MRVP) or
Peptone Glucose
Broth

Question 9

Useful in distinguishing
among members of the
family Enterobacteriaceae

Biochemical
Characterization
Medium

Answer 9

Methyl Red
Voges-Proskauer
Medium

Question 10

For the isolation of
Mycobacterium species

Selective

Answer 10

Middlebrook 7H9
(liq) / 7H10 / 7H11
(solid)

Question 11

For the isolation of
Mycobacterium species

selective

Answer 11

Mitchison agar

Question 12

For the isolation of Borrelia
spp.

selective

Answer 12

Modified Kelly’s
Medium or
Barbour-Stoenner-K
elly(BSK-II)

Question 13

For the recovery of
Neisseria gonorrhoeae
and Neisseria meningitidis
in a mixed microbiota

selective

Answer 13

Modified
Thayer-Martin Agar

Question 14

For the determination of a
motile from a non-motile
organism

Selective

Answer 14

Motility Test
Medium

Question 15

For the identification of
gram-negative enteric
bacilli based on the ability
to produce LDC (lysine
decarboxylase), ODC
(ornithine decarboxylase),
or ADH (arginine
dihydrolase)

Answer 15

Moeller’s broth

Question 16

For antibiotic susceptibility
test of most bacteria

selective

Answer 16

mueller-hinton agar

Question 17

For the isolation of
pathogenic spp. of
Neisseria such as
Neisseria gonorrhoeae
and Mycoplasma hominis,
and Ureaplasma
urealyticum

Selective

Answer 17

NYC

Question 18

Used to determine
whether an organism can
reduce nitrate to nitrite or
to glucose end products
such as nitrogen

Answer 18

Nitrate Reduction
Broth

Question 19

For the determination of a
non-fastidious organism
from a fastidious organism

basal medium

Answer 19

nutrient agar

Question 20

For the differentiation of
organisms based on their
ability to oxidize or
ferment carbohydrate

Answer 20

Oxidation-Fermenta
tion medium(O-F)

Question 21

For the identification of
Corynebacterium
diphtheriae

selective

Answer 21

Pai’s agar

Question 22

Useful for culturing
anaerobes

Enriched

Answer 22

Peptone-Y east
Extract-Glucose
Broth(PYG)

Question 23

For the isolation of
Mycobacterium spp.

selective

Answer 23

Petragnani medium

Question 24

For the identification of
gram-negative enteric
bacilli based on their
ability to produce enzyme
deaminase

Answer 24

Phenylalanine
Deaminase agar
(PDA)

Question 25

For the selective isolation
of gram-positive cocci and
anaerobic gram-negative
bacilli

Selective

Answer 25

Phenylethanol agar
(PEA)

Question 26

For the isolation of
Mycoplasma spp.

Answer 26

Pleuropneumonia-li
ke Organism Agar
(PPLO)

Question 27

For isolation of
Corynebacterium
diphtheriae

Selective,
Differential,
Enrichment

Answer 27

Potassium Tellurite
Blood Agar

Question 28

Differentiates
beta-hemolytic
streptococci and
enterococci based on their
ability to produce enzyme,
pyrrolidonyl arylamidase,
or pyroglutamyl
aminopeptidase

Biochemical
Characteristic
Medium

Answer 28

PYR agar

Question 29

For the isolation of
Bordetella pertussis

Answer 29

Regan Lowe agar

Question 30

For the isolation and
differentiation of
Salmonella and Shigella

Answer 30

Salmonella-Shigella
agar(SSA)

Question 31

For the identification of
organisms based on their
ability to grow or not grow
in the presence of 6.5%
NaCl

differential medium

Answer 31

Salt broth(NaCl)

Question 32

For the recovery of
aerobes and anaerobes

Answer 32

Schaedler agar

Question 33

For blood culture

biphasic medium

Answer 33

Scott’s modified
Castaneda and
Thioglycolate broth

Question 34

For enrichment and
isolation of Salmonella
spp.

enrichment

Answer 34

selenite broth

Question 35

For differentiating
non-fermentative bacilli
such as Pseudomonas,
Acinetobacter, Alkaligenes

differential

Answer 35

Seller’s agar

Question 36

For isolation of
Mycoplasma spp.

Selective

Answer 36

Shepard’s A7-B
agar

Question 37

For the identification of
gram-negative enteric
bacilli based on their ability to utilize citrate as a
source of carbon

Answer 37

Simmons Citrate
agar(SCA)

Question 38

For the isolation of
Campylobacter spp.

Selective

Answer 38

skirrows medium

Question 39

Useful in the differentiation
of streptococci;
distinguishes
Enterococcus spp. from
group D streptococci

Answer 39

Sodium Chloride
Broth, 6.5%

Question 40

Primary isolation medium
for Mycoplasma spp.

Answer 40

SP-4 Broth/Agar

Question 41

For the isolation of
Streptococcus pyogenes
and Streptococcus
agalactiae.

Answer 41

Streptococcal
Selective Agar
(SSA)

Question 42

For the isolation of
Salmonella and Shigella
spp. from stool samples.

enrichment

Answer 42

Tetrathionate Broth

Question 43

For isolation and
differentiation of Vibrio
spp.

Answer 43

Thiosulfate Citrate
Bile Salts Sucrose
Agar(TCBS)

Question 44

For the isolation of
Corynebacterium
diphtheriae.

Answer 44

tinsdale agar

Question 45

For enrichment and
isolation of Streptococcus
agalactiae.

enrichment and selective

Answer 45

Todd-Hewitt Broth
Supplemented with
Antibiotics

Question 46

For Neisseria
gonorrhoeae.

Answer 46

transgrow

Question 47

Supports the growth of
many nonfastidious and
fastidious bacteria.
Trypticase soy agar can be
used as a base in
preparing blood agar.

general purpose medium

Answer 47

trypticase soy broth agar

Question 48

For the identification of
gram-negative enteric
bacilli based on their
ability to produce indole.

Biochemical
Characteristic
Medium

Answer 48

tryptone broth

Question 49

Used for the Indole Test in
the identification of
Enterobacteriaceae
species and
non-glucose-fermenting,
gram-negative bacilli.

Answer 49

Tryptophan Broth,
1%

Question 50

Useful in identifying
species of
Enterobacteriaceae.

Biochemical
Characteristic
Medium

Answer 50

urea agar and broth

Question 51

Useful in the isolation of
Gardnerella vaginalis.

selective and enrichment

Answer 51

Vaginalis Agar

Question 52

For the isolation of
nontuberculous
mycobacteria such as
Mycobacterium avium.

selective

Answer 52

wallenstein medium

Question 53

For the identification of
gram-negative enteric
bacilli and for the
differentiation of
Salmonella and Shigella
spp.

Selective and
Differential

Answer 53

Xylose Lysine
Deoxycholate Agar
(XLD)