LIST OF CUTURE MEDIA Flashcards by FRANCHESCA MAUREEN CUISON

Selective and
Differential

For the isolation of
Escherichia coli

MacConkey Sorbitol
Agar

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Selective

For the identification of
Enterobacteriaceae
species, particularly
Salmonella

Malonate Broth

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Selective and
Differential

For isolation and
differentiation of mannitol
and non-mannitol
fermenting Staphylococci

Mannitol Salt agar
(MSA)

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Selective

For the isolation of
pathogenic Neisseria spp.
such as Neisseria
gonorrhoeae

Martin Lewis agar

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selective

For the isolation of Listeria
monocytogenes

mcbride medium

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Tissue cell culture

For the isolation of
Chlamydia

mccoy cells

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mcleod’s agar

For the identification of
Corynebacterium
diphtheriae

selective

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For the identification of
gram-negative enteric
bacilli based on their
ability to ferment glucose
via mixed acid
fermentation or butylene
glycol pathway

Methyl Red
Voges-Proskauer
Test(MRVP) or
Peptone Glucose
Broth

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Useful in distinguishing
among members of the
family Enterobacteriaceae

Biochemical
Characterization
Medium

Methyl Red
Voges-Proskauer
Medium

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For the isolation of
Mycobacterium species

Selective

Middlebrook 7H9
(liq) / 7H10 / 7H11
(solid)

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For the isolation of
Mycobacterium species

selective

Mitchison agar

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For the isolation of Borrelia
spp.

selective

Modified Kelly’s
Medium or
Barbour-Stoenner-K
elly(BSK-II)

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For the recovery of
Neisseria gonorrhoeae
and Neisseria meningitidis
in a mixed microbiota

selective

Modified
Thayer-Martin Agar

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For the determination of a
motile from a non-motile
organism

Selective

Motility Test
Medium

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For the identification of
gram-negative enteric
bacilli based on the ability
to produce LDC (lysine
decarboxylase), ODC
(ornithine decarboxylase),
or ADH (arginine
dihydrolase)

Moeller’s broth

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For antibiotic susceptibility
test of most bacteria

selective

mueller-hinton agar

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For the isolation of
pathogenic spp. of
Neisseria such as
Neisseria gonorrhoeae
and Mycoplasma hominis,
and Ureaplasma
urealyticum

Selective

NYC

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Used to determine
whether an organism can
reduce nitrate to nitrite or
to glucose end products
such as nitrogen

Nitrate Reduction
Broth

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For the determination of a
non-fastidious organism
from a fastidious organism

basal medium

nutrient agar

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For the differentiation of
organisms based on their
ability to oxidize or
ferment carbohydrate

Oxidation-Fermenta
tion medium(O-F)

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For the identification of
Corynebacterium
diphtheriae

selective

Pai’s agar

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Useful for culturing
anaerobes

Enriched

Peptone-Y east
Extract-Glucose
Broth(PYG)

For the isolation of
Mycobacterium spp.

selective

Petragnani medium

For the identification of
gram-negative enteric
bacilli based on their
ability to produce enzyme
deaminase

Phenylalanine
Deaminase agar
(PDA)

For the selective isolation of gram-positive cocci and anaerobic gram-negative bacilli Selective

Phenylethanol agar (PEA)

For the isolation of Mycoplasma spp.

Pleuropneumonia-li ke Organism Agar (PPLO)

For isolation of Corynebacterium diphtheriae Selective, Differential, Enrichment

Potassium Tellurite Blood Agar

Differentiates beta-hemolytic streptococci and enterococci based on their ability to produce enzyme, pyrrolidonyl arylamidase, or pyroglutamyl aminopeptidase Biochemical Characteristic Medium

PYR agar

For the isolation of Bordetella pertussis

Regan Lowe agar

For the isolation and differentiation of Salmonella and Shigella

Salmonella-Shigella agar(SSA)

For the identification of organisms based on their ability to grow or not grow in the presence of 6.5% NaCl differential medium

Salt broth(NaCl)

For the recovery of aerobes and anaerobes

Schaedler agar

For blood culture biphasic medium

Scott's modified Castaneda and Thioglycolate broth

For enrichment and isolation of Salmonella spp. enrichment

selenite broth

For differentiating non-fermentative bacilli such as Pseudomonas, Acinetobacter, Alkaligenes differential

Seller's agar

For isolation of Mycoplasma spp. Selective

Shepard's A7-B agar

For the identification of gram-negative enteric bacilli based on their ability to utilize citrate as a source of carbon

Simmons Citrate agar(SCA)

For the isolation of Campylobacter spp. Selective

skirrows medium

Useful in the differentiation of streptococci; distinguishes Enterococcus spp. from group D streptococci

Sodium Chloride Broth, 6.5%

Primary isolation medium for Mycoplasma spp.

SP-4 Broth/Agar

For the isolation of Streptococcus pyogenes and Streptococcus agalactiae.

Streptococcal Selective Agar (SSA)

For the isolation of Salmonella and Shigella spp. from stool samples. enrichment

Tetrathionate Broth

For isolation and differentiation of Vibrio spp.

Thiosulfate Citrate Bile Salts Sucrose Agar(TCBS)

For the isolation of Corynebacterium diphtheriae.

tinsdale agar

For enrichment and isolation of Streptococcus agalactiae. enrichment and selective

Todd-Hewitt Broth Supplemented with Antibiotics

For Neisseria gonorrhoeae.

transgrow

Supports the growth of many nonfastidious and fastidious bacteria. Trypticase soy agar can be used as a base in preparing blood agar. general purpose medium

trypticase soy broth agar

For the identification of gram-negative enteric bacilli based on their ability to produce indole. Biochemical Characteristic Medium

tryptone broth

Used for the Indole Test in the identification of Enterobacteriaceae species and non-glucose-fermenting, gram-negative bacilli.

Tryptophan Broth, 1%

Useful in identifying species of Enterobacteriaceae. Biochemical Characteristic Medium

urea agar and broth

Useful in the isolation of Gardnerella vaginalis. selective and enrichment

Vaginalis Agar

For the isolation of nontuberculous mycobacteria such as Mycobacterium avium. selective

wallenstein medium

For the identification of gram-negative enteric bacilli and for the differentiation of Salmonella and Shigella spp. Selective and Differential

Xylose Lysine Deoxycholate Agar (XLD)