unit 2: steps of glycolysis Flashcards
glycolysis step 1 inputs
glucose, ATP
glycolysis step 1 outputs
glucose 6-phosphate, ADP
glycolysis step 1 facilitative enzyme
kinase
glycolysis step 1 function
phosphorylation of glucose (phosphate comes from ATP molecule)
glycolysis step 2 inputs
glucose 6-phosphate
glycolysis step 2 outputs
fructose 6-phosphate
glycolysis step 2 facilitative enzyme
isomerase
glycolysis step 2 function
reconfiguring the carbon based molecule to make a new isomer
glycolysis step 3 inputs
fructose 6-phosphate, ATP
glycolysis step 3 outputs
fructose 1,6-bisphosphate, ADP
glycolysis step 3 facilitative enzyme
kinase
glycolysis step 3 function
phosphorylation of fructose 6-phosphate (phosphate from ATP molecule); creates a high energy molecule
glycolysis step 4 inputs
fructose 1,6-bisphosphate
glycolysis step 4 outputs
2 new molecules: glyceraldehyde 3-phosphate
glycolysis step 4 facilitative enzyme
aldolase
glycolysis step 4 function
breaking of the high energy molecule (fructose 1,6-bisphosphate) into 2 new G3P molecules
glycolysis step 5 inputs
dihydroxyacetone phosphate (DHAP)
glycolysis step 5 outputs
glyceraldehyde 3-phosphate (G3P)
glycolysis step 5 facilitative enzyme
isomerase
glycolysis step 5 function
converts DHAP into G3P, creating the 2nd G3P molecule
steps 1-5 of glycolysis are considered the _____ phase
the energy investment phase
how many ATP are used in steps 1-5 of glycolysis?
2
glycolysis steps 6-10 are considered the _______ phase
the energy payoff phase
how many ATP are made in steps 6-10 of glycolysis?
4 (net 2 after considering the energy investment phase)
how many new ATP are created after glycolysis?
4 (net 2 after considering the energy investment phase)
glycolysis step 6 inputs
glyceraldehyde 3-phosphate; NAD+ (2x per 1 glucose molecule)
glycolysis step 6 outputs
1,3-bisphosphoglycerate; NADH (2x per 1 glucose molecule)
glycolysis step 6 facilitative enzyme(s)
kinase and dehydrogenase
glycolysis step 6 function
phosphorylation and dehydrogenation of G3P; NAD+ is reduced to NADH
glycolysis step 7 inputs
1,3-bisphosphoglycerate; ADP (2x per 1 glucose molecule)
glycolysis step 7 outputs
3-phosphoglycerate, ATP (2x per 1 glucose molecule)
glycolysis step 7 facilitative enzyme
kinase
glycolysis step 7 function
phosphorylation of ADP (phosphate comes from 1,3-BPG); this creates 2 ATP per 1 glucose molecule to offset the ATP used in steps 1-5
glycolysis step 8 inputs
3-phosphoglycerate (2x per 1 glucose molecule)
glycolysis step 8 outputs
2-phosphoglycerate (2x per 1 glucose molecule)
glycolysis step 8 facilitative enzyme
isomerase
glycolysis step 8 function
move phosphate to a more reactive position on the molecule (creating another isomer)
glycolysis step 9 inputs
2-phosphoglycerate (2x per 1 glucose molecule)
glycolysis step 9 outputs
phosphoenol-pyruvate (PEP; H2O (2x per 1 glucose molecule)
glycolysis step 9 facilitative enzyme
enolase
glycolysis step 9 function
removed H2O from each 2-phosphoglycerate molecule to create even less stable PEP molecules
glycolysis step 10 inputs
phosphoenol-pyruvate (2x per 1 glucose molecule)
glycolysis step 10 outputs
pyruvate; ATP (2x per 1 glucose molecule)
glycolysis step 10 facilitative enzyme
kinase
glycolysis step 10 function
phosphorylation of ATP molecules; form the final 2 pyruvate molecules from every 1 starting glucose molecule
glycolysis overall reactants and products
C6H12O6 (glucose) + 2 ATP + 2 NAD+ -> 2 pyruvate + 4 ATP + 2 NADH
glycolysis overall rxt to prod: steps 1-5
1 glucose molecule (6C) + 2 ATP -> 2 G3P molecules (2x 3C) + 2 ADP
glycolysis overall rxt to prod: steps 6-10
2 G3P molecules + 2 NAD+ + 4 ADP -> 2 pyruvate molecules + 2 NADH + 4 ATP + 2 H2O